Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,876,678	IS5 (+) +4 bp	coding (98‑101/1038 nt)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	54 (0.740)	45/282	0.7	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2876678 =	0 (0.000)					coding (98/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
*	?	NC_000913	= 2290113	NA (NA)	68 (0.930)	51/282	0.4	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 2876681	0 (0.000)					coding (101/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion

CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289048‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGT  >  NC_000913/2876678‑2876817                                                                                                                                                                                                                                                                                CTTAAATAGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCCGGC                                                                                                                                 >  1:476181/1‑143      acGGCGAAGAAACGATATAAAGAGCGTGATTCAAGGCATTGACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGAC                                                                                                                            <  2:795264/141‑1       ttGCGAAGAAACGGTATAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACA                                                                                                                           <  1:464889/141‑1         GCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTCACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCCCGcccta                                                                                                                         >  1:738048/1‑138           GAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACAACATAct                                                                                                                       >  1:233996/1‑141                cACGGTCTAACTATGGTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAGTTACTGAGTCAGCCGAGAAGAACTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTAC                                                                                                                  <  1:1107794/142‑1                  CGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTAATAGCAACATCCCTAATACTCAGGCACGACATATTGCTACTT                                                                                                                >  1:199466/1‑143                   GGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTT                                                                                                               <  2:490944/143‑1                    GTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCCCCTTCCCTCAGACTCAGGCACGACCTATTCCTACTTca                                                                                                              >  2:898182/1‑141                    GTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGCCATATTGCTCCTTTC                                                                                                              >  2:225277/1‑143                    GTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTAAGGCACGACATATTGCAACTTTg                                                                                                              >  2:896783/1‑142                           gagcCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGG                                                                                                       <  2:333853/139‑1                           TAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGG                                                                                                       <  1:151684/143‑1                               CTGATTCAAGGCATTTACGGGAGAAATAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACG                                                                                                   <  1:407101/143‑1                                   TTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATG                                                                                               >  1:163728/1‑143                                    TCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGA                                                                                              <  2:1159458/143‑1                                      AAGGCATTTACGGGAGAAAAAATCGGCTCAAATATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTACCTAATACTCAGGCAAGACATATTGCTACTTTCTTACCGAGACGCATGACC                                                                                            >  2:688359/1‑143                                      AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACC                                                                                            <  1:215732/143‑1                                      AAGGCATTTACGGGAGAAAAAATCGGCTCAAACATAAAGAAATGAAATGACTGAGTCAGCCGAAAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTATGTTTCAGGGAAGCAgaaac                                                                                            >  2:942500/1‑138                                       AGGCATTTACGGGAGAAAAAATCGGCTCAACCATGAAGAAATGAAATGACTGAGGCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATAGCCACTTGCCGTCCCGGAGGCATGCCCG                                                                                           >  1:471580/1‑143                                           ATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAAC                                                                                       >  2:205132/1‑143                                              TACGGGAGAAAGATTCGGCTCAAACATGAAGAAATGACATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCTCTCCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCC                                                                                    <  2:143984/143‑1                                                 GGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGC                                                                                 <  1:1277803/143‑1                                                 GGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGC                                                                                 <  1:459491/143‑1                                                       AAAGATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACCTATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAAT                                                                           <  2:650561/143‑1                                                        AAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATG                                                                          <  2:1302114/143‑1                                                           ATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACACACCGTCTTTTGCTGGCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTA                                                                       >  1:603147/1‑143                                                            TCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCCGGACGCCTGACCGGAACTCGTGCCGAAATGTTct                                                                      >  2:1298310/1‑141                                                              GGCTCAAACATGAAGAAATGAAATGCCTGAGTCAGCCGAGAAGAATCTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCT                                                                    <  2:968465/143‑1                                                                 TCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCTCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCC                                                                 >  2:1281520/1‑143                                                                 TCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGCAGCATGACCGGAACCCCTGCAGACATGTTctacgca                                                                 >  2:684170/1‑136                                                                    AACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGAT                                                              <  1:865974/143‑1                                                                     ACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATT                                                             <  1:657259/143‑1                                                                     ACATCAAGAATTGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATT                                                             <  2:72304/143‑1                                                                             AAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGC                                                     <  1:205132/143‑1                                                                              AATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACGCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCC                                                    <  2:1079217/143‑1                                                                                TGAAATAACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAGACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTTCAGAAATGTTATCTGCCGATTATATTCGCCAA                                                  <  1:310946/143‑1                                                                                    ATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCGCACAGT                                              >  2:792499/1‑143                                                                                     TGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCGGCAATGTTATCTGCCGATTCTATTCGCCAACAGTT                                             >  1:533734/1‑143                                                                                        CTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCA                                          >  1:443125/1‑143                                                                                         TGAGTCAGCCGAGAAGAATTTCCCCGCTTATTAGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTACGGGTCGCATGACCGGCACTCCTGCAGAAATGTTATCTGTTGATGATATTGGACAACAGTTTAAG                                         >  2:329755/1‑143                                                                                               AGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATG                                   <  1:270224/143‑1                                                                                                GCCGAGAAGACTTTCCCCGCTTATTCGCACCTTCCCTCATACCCAGGCACGACATATTGCTACTTTCTTTCCGGGTCGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGG                                  <  2:420922/143‑1                                                                                                GCCGAGAAGAATTTCCCCGCTTAGTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGG                                  <  1:730722/143‑1                                                                                                GCCGAGAAGAATTGCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTTGCCAACAGTTTCAGCAAATGG                                  <  2:824423/143‑1                                                                                                   GAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCAACAAGAATTACAGCAAcaggacg                               >  2:205900/1‑136                                                                                                         AATTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCA                         <  2:163728/143‑1                                                                                                           tgTCCCCGCTTATTTGCACCTTCCCTAATGCTCAAGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGT                       <  2:233996/141‑1                                                                                                           TTTCCCCGCTTATTCGCACCTTCCCTAATACTCAGGCCCGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGTACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACACTTTCAGCACATGGGTTATCGCAct                       >  2:222706/1‑141                                                                                                              CCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGTGAT                    <  1:340402/143‑1                                                                                                              CCCCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGGTTAAGCAACCGGCATATCGCAGTGAT                    >  1:991025/1‑143                                                                                                                CCGCTTATTCGCACCTTCCCTAATACTCAGGCACGACATATTCCTACTTTCTTTCCGGGACGCATGACCGCACCTCCTGCAGAAGTGTTATATGCCGATTATATCCGCCAACACTTTCAGCAAATGGGTTATCGCCGTGACAT                  >  2:1164146/1‑143                                                                                                                              CTTCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCCGTGATATTCGGACCTTTcgtg    >  1:129040/1‑139                                                                                                                                TCCCTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAat  >  2:98275/1‑141                                                                                                                                                                                                                                                                                CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289048‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CTAATACTCAGGCACGACATATTGCTACTTTCTTTCCGGGACGCATGACCGGAACTCCTGCAGAAATGTTATCTGCCGATTATATTCGCCAACAGTTTCAGCAAATGGGTTATCGCAGTGATATTCGGACATTTAATAGT  >  NC_000913/2876678‑2876817

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.