Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,979,486	IS5 (+) +4 bp	intergenic (‑271/‑264)	insA ← / → uspC	IS1 repressor TnpA/universal stress protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1979486 =	1 (0.030)	61 (1.640)	40/284	0.0	98.3%	intergenic (‑271/‑267)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1979489	1 (0.030)	65 (1.750)	32/284	0.2	98.4%	intergenic (‑274/‑264)	insA/uspC	IS1 repressor TnpA/universal stress protein
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA  >  NC_000913/2101607‑2101884                                                                                                                                                |                                                                                                                                        tCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATGTACACGC‑CACTTTCCTCGTTGACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaag                                                                                                                                         <  1:490287/143‑1 (MQ=255)   ttCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACATGCATAAAGGACTCTGCCCACGCAGCATCAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaagga                                                                                                                                       <  2:298129/143‑1 (MQ=255)      acaGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGACTAGATACTTTCTCTACGAATTTTTGTTTTATTaagaaaga                                                                                                                                    >  1:203135/1‑142 (MQ=255)       caGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTAAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGt                                                                                                                                   >  1:557967/1‑143 (MQ=255)         gTACATAAAGTAACCTTGATCGAAGCCATTTACGCGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGc                                                                                                                                 <  1:117855/143‑1 (MQ=255)            cATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGaa                                                                                                                              >  1:526387/1‑143 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATCGATACTTTCTTTAGGAATTTTTGATTTATTAAGGAAGGTGCGAATAAGCGGGGaa                                                                                                                   >  2:22693/1‑143 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGaa                                                                                                                   >  1:7184/1‑143 (MQ=255)                       cTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTATATAGGAGGCATAATTTTATTAAGTAAGGTGCGAATAAGCGGGGaa                                                                                                                   >  2:294627/1‑143 (MQ=255)                              gAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTATATTAATGAAGGTGCGAATAAGCGGAGAACttcttc                                                                                                            >  2:346516/1‑143 (MQ=255)                                    aTTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGCATTTTTGTTCTATTAAGGATGGTGTGAATAAGCGCGGAAATTCTTCTCGGCt                                                                                                      >  1:181018/1‑143 (MQ=255)                                       tACACGCACAAAAT‑CTGAAAAACGTACCAGCATCAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGAc                                                                                                   <  2:416494/143‑1 (MQ=255)                                                cacgagcTGAACAACGTACCAGCATAAATGATCCGGCGCACCAATCAACAACCGTATCAGAATAGATACTGTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcat                                                                                           <  2:603308/137‑1 (MQ=38)                                                aaaaC‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                          <  2:526387/143‑1 (MQ=255)                                                 aaaT‑CTGAAAAGCGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAAGAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcattt                                                                                         <  2:497090/143‑1 (MQ=255)                                                        gAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCatttcatttc                                                                                   <  1:178272/143‑1 (MQ=255)                                                               gTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGt                                                                            <  1:292223/143‑1 (MQ=255)                                                                tACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTCAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGCAATTCTTCTCGGCTTACTCAGTCGCCTAACTGCTTCATGtt                                                                           >  2:398920/1‑143 (MQ=37)                                                                 actaGCATAAATGATCCTGCGCACCAATCAACTACGGGAACACAATAGATACTTTCTTTAGGAAGTTTTGTTTGATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGttt                                                                          <  2:138028/140‑1 (MQ=21)                                                                   cAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                        <  1:22586/143‑1 (MQ=255)                                                                     gCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                      >  1:64486/1‑143 (MQ=255)                                                                     gCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                      <  2:83474/143‑1 (MQ=255)                                                                     gCATAAACGATCCTGCGCACCACTCAACAACAGTATCAGAATAGCTACTTTCTTTAGGAATTTTTGTTTTATTAACGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                      <  2:210430/143‑1 (MQ=25)                                                                         aaaTGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGaa                                                                  >  2:5313/1‑142 (MQ=255)                                                                              aTCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTCGAGCCGAtttttt                                                             >  2:122819/1‑143 (MQ=255)                                                                              aTCCTGCGCACCAATCAACAACAGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                             <  1:42869/143‑1 (MQ=255)                                                                               tCCTGCGCACCAATCAACAACCGTAGCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                            >  1:587232/1‑143 (MQ=255)                                                                                ccTGTGCACCAATCAACAACCGTATCAGAAAAGAGACGTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                                                           <  1:179176/143‑1 (MQ=255)                                                                                 gtGCGCACCATGCAACAGCCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                          <  2:133825/142‑1 (MQ=255)                                                                                 cctcgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                          <  1:517851/140‑1 (MQ=255)                                                                                 cTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                          >  1:628870/1‑143 (MQ=255)                                                                                 cTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtctc                                                          <  2:151033/143‑1 (MQ=255)                                                                                    cgcACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCg                                                       <  1:294627/143‑1 (MQ=255)                                                                                         cAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                  <  1:496463/143‑1 (MQ=255)                                                                                         cAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                  >  2:172225/1‑143 (MQ=255)                                                                                         cAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCAGATTTTTTCTCCCGTAAAt                                                  <  1:303345/143‑1 (MQ=255)                                                                                         cAATCAACAACCGTAACAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                  <  1:302511/143‑1 (MQ=255)                                                                                             caacaaGCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCt                                              <  1:518873/143‑1 (MQ=255)                                                                                                    cGTATCAGAATAGATACTGTGTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                       <  1:540736/143‑1 (MQ=255)                                                                                                            aaTAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                               <  1:353361/143‑1 (MQ=255)                                                                                                               tgATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGa                            <  2:465577/142‑1 (MQ=255)                                                                                                                 aTACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTTTCCCGTAAATGCCTTGAATTCGCCTATTTAGAcc                          >  2:19229/1‑143 (MQ=255)                                                                                                                   aCTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTATTTCTTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACTGt                        >  1:602698/1‑143 (MQ=17)                                                                                                                            aGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGATACTTCGcc               >  1:334939/1‑143 (MQ=255)                                                                                                                                   tttGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAg        <  1:556747/143‑1 (MQ=38)                                                                                                                                       ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCCTATTTAGACCGTTTCTTCGCCCTTTCAAGCGt    >  1:290356/1‑143 (MQ=255)                                                                                                                                       ttttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTACGGcag    >  2:332315/1‑141 (MQ=21)                                                                                                                                        tttATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAGATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGtt   >  2:463524/1‑143 (MQ=18)                                                                                                                                         ttattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTa  >  2:542486/1‑143 (MQ=32)                                                                                                                                                |                                                                                                                                        TCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAAT‑CTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTA  >  NC_000913/2101607‑2101884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.