Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,876,678	IS5 (+) +4 bp	coding (98‑101/1038 nt)	iap →	aminopeptidase in alkaline phosphatase isozyme conversion

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	32 (0.860)	31/284	0.2	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	2876678 =	0 (0.000)					coding (98/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion
*	?	NC_000913	= 2290113	NA (NA)	29 (0.780)	28/284	0.3	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 2876681	0 (0.000)					coding (101/1038 nt)	iap	aminopeptidase in alkaline phosphatase isozyme conversion

TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATC  >  NC_000913/2288778‑2289056                                                                                                                                              |                                                                                                                                          ttttATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaagg                                                                                                                                          >  2:178778/1‑143 (MQ=255)     atatGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGATGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATaaggaagg                                                                                                                                      <  2:344139/143‑1 (MQ=255)              tctcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCCCCATCCTCAACAGTTGATGCAAATGCTATGGGTGAATATAAGGAAGGTGCGAATaa                                                                                                                             >  1:236907/1‑143 (MQ=255)                  cGCCAGGAGTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGCGAATATAAGGAAGGTGCGAATAAGCgg                                                                                                                         >  2:15296/1‑143 (MQ=255)                   gCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCggg                                                                                                                        >  1:636282/1‑143 (MQ=255)                        gattgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                   >  1:137997/1‑143 (MQ=255)                        gattgattTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAAAACCATCAACTGTCTCACCATACTCCACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAt                                                                                                                   >  1:270684/1‑143 (MQ=255)                         cttgattATCGTATTATTGGCGGTCCCATCAACCAGCTGGATCTCTTTTTCCAGGACACCCCCCTCTACTGTCTCACCATCCCCACCCGATGATGCCCATGCTCTGAGTACCTATAAGGCAGGTGAGAATCAGAGTGTAAAtg                                                                                                                  >  2:139595/2‑142 (MQ=255)                            gattTTCGTATTATTGGCAGTCCCATAAACCAGCTGGATATCTTTTTCCAAGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAttctt                                                                                                               <  1:215854/143‑1 (MQ=255)                             attTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAAttcttc                                                                                                              >  1:367743/1‑143 (MQ=255)                                 tCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                          <  1:463564/143‑1 (MQ=255)                                    tattatTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTg                                                                                                       >  1:534502/1‑143 (MQ=255)                                        attGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGACTGACTc                                                                                                   >  2:83275/1‑143 (MQ=255)                                               gTCCCATCAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatt                                                                                            <  2:351880/143‑1 (MQ=255)                                                   cATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttcat                                                                                        >  1:197613/1‑143 (MQ=255)                                                        aCCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATttctt                                                                                   <  2:151625/143‑1 (MQ=255)                                                            gCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGCGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATg                                                                               >  2:154417/1‑143 (MQ=255)                                                                 agatCTGTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGGGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGa                                                                          <  1:673973/141‑1 (MQ=255)                                                                   atCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGACTCTAAGGAAGGTGCGAATAAGTGCGGAAATTCTTCTCGGCTGACTACGTAATTTCATTTCTTCATGTTTGAGc                                                                        >  2:619430/1‑143 (MQ=25)                                                                    tCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTGCTCGGCTGACTCAGTCATTTCAGTTCTTCGCGTTGGAGcc                                                                       >  1:225880/1‑143 (MQ=37)                                                                     cTTTTTCCAGGACAACACCATCAACTGTCTCACCCTACTCAGCGGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTGTCGGCTGACTCAGTCATTTCATTGGTGCATGTTTGacgcg                                                                      >  2:468431/1‑139 (MQ=25)                                                                       ttttCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGATATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTAGGCAGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAt                                                                    >  2:651434/1‑143 (MQ=255)                                                                              ggACACCACCATCAACTGTCTCACCATCCTCAACTGTTGATGCCACTGCTATGGGTGAATATAAGGAAGGTGCGAATATGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtct                                                             <  2:66083/143‑1 (MQ=21)                                                                                  aacaCCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTAGGCTGACTCAGTCATTTCATTTCTTCATGTTTTAGCCGCTTTTTTCTCCCg                                                         >  2:66757/1‑143 (MQ=255)                                                                                       cATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAACATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAAt                                                    >  2:123673/1‑143 (MQ=255)                                                                                        aTCAACTGTCTCACCATACTCAACAGTTGATGCCACTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGGTTGGGGCGGTATTTGCTCCCGTAAATg                                                   >  1:50574/1‑143 (MQ=21)                                                                                        aTCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATg                                                   >  1:97563/1‑143 (MQ=255)                                                                                                tctcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTGAAt                                           >  2:355278/1‑143 (MQ=255)                                                                                                  tcACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCa                                         >  1:351390/1‑143 (MQ=255)                                                                                                   cACCATACTCATCAGTTGATGCCAAGGCCATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                        <  1:171462/143‑1 (MQ=255)                                                                                                    aCCATACTCAACAGTTGATGCCAATGCTATGGGTGCACATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                       >  2:34926/1‑143 (MQ=255)                                                                                                     ccATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGACTTGAATCAGcc                                      >  2:58020/1‑143 (MQ=255)                                                                                                         aCTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAtt                                  <  1:501464/143‑1 (MQ=255)                                                                                                          cgcAACAGTTGATGCCAAGGCTATGGGTGAAGAGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                                 <  1:619430/141‑1 (MQ=255)                                                                                                             aaCAGTTGATGCCAATGCTATGGGTGAATATACGGAAGGTGCGAATAAGCGGGGAACTTCTTCTCGGATGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAACTCCATTGCAGAAGCCTCCTTAGa                              >  2:177603/1‑143 (MQ=17)                                                                                                                 gctGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                          <  1:494720/141‑1 (MQ=255)                                                                                                                 gTTGATACCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTATTCATGTTTGAGCAGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGt                          <  1:254571/143‑1 (MQ=255)                                                                                                                        tcAATGCTATGGGTGAATATAATGAAGGTGTGGTTTCGCGTAAAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                   <  2:137997/142‑1 (MQ=14)                                                                                                                        ccAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGCCTCAGTACTTTCATTTCTTCATGTTTGAGCCGAGTTTTTCTCCCGTCTTCGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                   <  2:270684/143‑1 (MQ=14)                                                                                                                        ccAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                   <  1:406686/143‑1 (MQ=255)                                                                                                                         cAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCATTGAATCAGCCAATTTAGACCGTTTCTACga                  >  2:83912/1‑142 (MQ=255)                                                                                                                          aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGcc                 <  1:296325/143‑1 (MQ=255)                                                                                                                          aaTGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCGATTTAGACCGCTTCTGCGcc                 >  2:152427/1‑143 (MQ=255)                                                                                                                                    gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAGTCAGCCTATTTATGCCGTTTCTTCGCCCTTTAAGGCg       >  1:420367/1‑143 (MQ=255)                                                                                                                                    gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATGCGCCTATTTAGACCGATTCTTCGCCATTTAAGGCg       >  1:272699/1‑143 (MQ=255)                                                                                                                                    gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg       >  1:481300/1‑143 (MQ=35)                                                                                                                                    gTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTAATAACGTGCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCg       <  1:668063/143‑1 (MQ=11)                                                                                                                                        atatAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTGTCCCGTAACTGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCCCTTAAGGCGTTAt   >  2:330378/1‑143 (MQ=255)                                                                                                                                         tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTTTGCCGCAAATTCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATc  >  1:491000/1‑143 (MQ=9)                                                                                                                                         tataAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTACATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCGTTTAAGGCGTTATc  >  1:590336/1‑143 (MQ=14)                                                                                                                                              |                                                                                                                                          TTTTATATGCTGTTCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATC  >  NC_000913/2288778‑2289056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.