Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,132,787 A→C I204S (ATC→AGC)  wcaA ← putative glycosyl transferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,132,7870AC97.9% 141.4 / NA 48I204S (ATC→AGC) wcaAputative glycosyl transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base C (26/21);  minor base G (0/1);  total (26/22)
Fisher's exact test for biased strand distribution p-value = 4.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926
                                                                                                                                         |                                                                                                                                           
tCTGGTGGATGTTATACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGTGGTAAAAGTGGAAGTACCCTGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  2:777177/143‑1 (MQ=255)
tCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGc                                                                                                                                        <  1:442455/143‑1 (MQ=255)
   ggTAGAGTGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTc                                                                                                                                     <  2:777805/143‑1 (MQ=255)
    gTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCg                                                                                                                                    >  2:791842/1‑143 (MQ=255)
     tAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGc                                                                                                                                   <  2:936459/143‑1 (MQ=255)
             aaaCAGTTGATCTTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTGGCGGTAAAAGTGGAAGTACCCGGAGAATTTTGTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTa                                                                                                                           <  1:698913/143‑1 (MQ=255)
                cAGCTGATGTTTTCGGCTGGCTCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACtt                                                                                                                        <  2:332604/143‑1 (MQ=255)
                 aGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGCGAATTTTTTCGGTGACGAGGTGATCTGCATTTAACCGTGATCGATATACAGGCACTGCTTCGCCTCTTCTACttt                                                                                                                       >  2:61095/1‑143 (MQ=255)
                  gcggATATTGTTGGGTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTc                                                                                                                      <  2:909532/140‑1 (MQ=255)
                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCCTTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  1:954138/1‑143 (MQ=255)
                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  1:239784/1‑143 (MQ=255)
                    tGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCa                                                                                                                    >  1:723969/1‑143 (MQ=255)
                      gtgttgtttGCTGGCGCGGTCGAATTTGTCTTTGGTCTGGCGGTAAAAGTGGAAGTGCCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACg                                                                                                                  <  2:919910/137‑1 (MQ=255)
                          tttttGCTGGCGCGTTCGAATTTGTCTTTGTGGTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTc                                                                                                              <  2:310913/143‑1 (MQ=255)
                           ttttGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCCGGCCCTGCGTCGGACCATCCACTTTCCCCGGTTCg                                                                                                             >  2:386540/1‑143 (MQ=255)
                               gCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTAATATGCAGGCTCTGCGTCGCCTCGTCTACTTTTCACGGTTCGCCGt                                                                                                         >  1:451375/1‑143 (MQ=255)
                                cTGGCGCGGTCGAAGTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTa                                                                                                        <  1:400450/143‑1 (MQ=255)
                                 tGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATATGACTTTCAGCTTGATGGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTGCACGGGTCGCCGCAc                                                                                                       >  2:859442/1‑143 (MQ=255)
                                       ggTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGCTATGCAGGCTGTGCGTCGCCTGTTCTACTTTCCCGGGTTCGCCGTTCTgcacc                                                                                                 >  1:243420/1‑143 (MQ=255)
                                                 gTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCa                                                                                       <  1:190200/143‑1 (MQ=255)
                                                               ggTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGGTTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACAATCCGCAGGCAGATTTCGTat                                                                         >  1:47328/1‑142 (MQ=255)
                                                                 tAAAAGTGGAAGTACTGGGAGAATGTTTTTGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATc                                                                       <  2:776248/143‑1 (MQ=255)
                                                                 tAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGGTATTGATATGCAGGCTCTGCGGCGCCTCATCTACTTTCCACGATTAGCCGGACTAGACCCCCACCCGCAGGCAGATGCCGtcacc                                                                       >  2:46329/1‑139 (MQ=255)
                                                                     aGTGGAGGTACCCGGAGAATCTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCtgt                                                                   <  1:328182/143‑1 (MQ=255)
                                                                                 cggacgaTTTTTCCGGTGCCGTGGTCATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTATTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGt                                                       <  2:456996/137‑1 (MQ=255)
                                                                                  ggAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGtt                                                      <  2:58325/143‑1 (MQ=255)
                                                                                     gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCgg                                                   >  1:973974/1‑143 (MQ=255)
                                                                                     gaATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAAGCCTGTGCCGCTTTCAGTGCgg                                                   >  2:266228/1‑143 (MQ=255)
                                                                                      gaTTTTTTCGGTGACGGGGTCGTCGGCATTTCGCCGTGGTTGATATGCAGGGCCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTTCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGt                                                  <  2:284849/142‑1 (MQ=255)
                                                                                      aaTTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCTCCACCCTCCGCCGTAACCTGTCGTCATCCTGTGCCGACTTCCGTTCGGt                                                  >  2:984111/1‑143 (MQ=255)
                                                                                         tttttCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATc                                               <  1:666632/143‑1 (MQ=255)
                                                                                            tgcGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaa                                            <  1:497286/141‑1 (MQ=255)
                                                                                              cGGTGACGACGTTTTCTTCTTTTCTCCTTTATTTATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGaaca                                          <  1:216918/143‑1 (MQ=255)
                                                                                                gTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGACGTCGTAATCCTCTGCCGCTTCCAGTTCCGTACCGaacaac                                        >  2:627342/1‑142 (MQ=255)
                                                                                                   aCGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAacac                                     >  1:230218/1‑143 (MQ=255)
                                                                                                        gTGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTCCTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCACCTTTCAGCTCGGTATCGAACCAACACTCttt                                >  1:654811/1‑143 (MQ=255)
                                                                                                         tGATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTg                               >  1:253675/1‑143 (MQ=255)
                                                                                                          gATCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCCTCCGCCGGAAGATATCCTAATCCTGTGCCGCTGTCAGTTCGGTATCGAACAGAGACCCTTTGa                              >  2:797325/1‑143 (MQ=255)
                                                                                                           aTCTGCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGCAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGaa                             >  2:654742/1‑143 (MQ=255)
                                                                                                               gCATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCCCGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTACTCCTGCGCCGCTTTCAGTTCGCGATGGAAACAACACCCTTTGGAACGc                         >  2:834923/1‑143 (MQ=255)
                                                                                                                cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCCCCATCAGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGcc                        <  2:478951/143‑1 (MQ=255)
                                                                                                                cATTTCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCAACACCATCCGCAGGAAGATGGCGTACTCCTGTGCCGCTTTCAGCGCGGTATCGAACAAACACTCTTTGAAACGcg                        >  2:373047/1‑142 (MQ=255)
                                                                                                                  tttCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCAACCACCCTCCGCAGGAAGATGTCGTAACCCTGTGCCGCTTTCAGTTCGGCATCGTCCAAACCCCACTTGACACGCCAt                      >  2:242825/1‑143 (MQ=255)
                                                                                                                    tCACCGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGc                    <  2:954138/143‑1 (MQ=255)
                                                                                                                        cGTGATTGATATGCAGGCTCTGCGTCGCCTCTTCTACTCTCCACGGTTCGCCGTACTCCACCCCCATCCGCCGGCAGATGTCGTAATCCTGTGCCGCTTTCAGCTCGGTATCGCACCAACCCTCTTTGAAACGCCATGccggg                >  2:216357/1‑140 (MQ=255)
                                                                                                                             ttgatATGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTaaa           <  1:359585/143‑1 (MQ=255)
                                                                                                                                atatGCAGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCGCGACGATCCGCAGGAAGATGTCGTACTCCTGTGCCGCTGTCAGTGCGGTATGGGACAAACCCTGTTTGTAAGGCCCTGCACCGGtggaggc        >  1:203198/1‑137 (MQ=255)
                                                                                                                                      aGGCTCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGCAACGAACACACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGAtt  >  2:554091/1‑143 (MQ=255)
                                                                                                                                         |                                                                                                                                           
TCTGGTAGAGGGTAAACAGCTGATATTTTTTGCTGGCGCGGTCGAATTTGTCTTTGTGCTTGCGGTAAAAGTGGAAGTACCCGGAGAATTTTTTCGGTGACGAGGTGATCTGCATTTCACCGTGATTGATATGCAGGATCTGCGTCGCCTCTTCTACTTTCCACGGTTCGCCGTACTCCACCACCATCCGCAGGAAGATGTCGTAATCCTGTGCCGCTTTCAGTTCGGTATCGAACAAACACTCTTTGAAACGCCATGCCCAGGTAAAGACCTGGTT  >  NC_000913/2132650‑2132926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑