Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,560,455	+G	intergenic (‑2/+1)	glpR ← / ← glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,560,455	1	.	G	100.0%	142.1 / NA	45	intergenic (‑2/+1)	glpR/glpR	pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (19/26);  total (19/26)

TCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560327‑3560587                                                                                                                                  |                                                                                                                                     tCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                         <  1:911779/143‑1 (MQ=255) gcGAGCCATTGGGGTTTTGCTACGCCACTTTGCGGGCGCTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGttt                                                                                                                         <  2:83642/142‑1 (MQ=255)   gAGCCATTTGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTg                                                                                                                       <  1:303807/143‑1 (MQ=255)         ttGGGGAGTTGCGCCGCCGGTTGGCGGGGTTTGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGc                                                                                                                 <  2:586766/143‑1 (MQ=255)            gggATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAg                                                                                                              <  1:260458/143‑1 (MQ=255)                  tGCCCCGCCACCTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                        <  1:950400/143‑1 (MQ=255)                  ggtgccGCGACTGTGGGGGGGCTGCGCTCTTTTGCGTCGGTCTGTGTGGCCGTGCGATCGTGCCACGGCGTGTGAACCGAACGGGAAGGCAGCGGCGCACCGGCAGGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCAtt                                                                                                        <  2:257347/139‑1 (MQ=255)                    cTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCCGCTCACTGa                                                                                                      >  1:215529/1‑143 (MQ=255)                    cTCCGCCACCTTGCCGCCGATGCGCTCGTTTTCTTCGGCCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGa                                                                                                      <  1:768607/143‑1 (MQ=255)                     tCCGCCGCTTTGCGGGCGATGCGCTCTGTTTCTTCGGTCTGGGTGGCCTTGCGATCGCGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                     <  2:125880/143‑1 (MQ=255)                     gccGCCACTGGGCGGGCGGTGCGCGCTGTCTCTTCGGGCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAg                                                                                                     <  1:75783/142‑1 (MQ=255)                           aCTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCGACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTGTGCTCCGCCCGCTCATGGAGGAcgcg                                                                                               >  2:550995/1‑143 (MQ=255)                             tttGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGGACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCCCATTGAGGTCgcggc                                                                                             >  1:349107/1‑143 (MQ=255)                             tttGCGGGCGAGGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCgcggc                                                                                             <  1:441554/143‑1 (MQ=255)                                gCGGGCTGTGCGCTCTTTTTCTTCGGGCTGGGTGGCCTTGCGATCGTGCCTCGGCGTGTTAATCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGaa                                                                                          <  2:46149/143‑1 (MQ=255)                                 cGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAGCTGGAAGGCAGCGCCGCACCGCCAGGATGGCGCAGGATCAGGTTTTGCCCCGCCAGCTCCATGAGGTCGCGGCGagt                                                                                         >  2:154133/1‑141 (MQ=255)                                  gggCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATa                                                                                        >  2:272068/1‑143 (MQ=255)                                    gCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGt                                                                                      >  2:40002/1‑143 (MQ=255)                                        tGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGc                                                                                  >  1:537837/1‑143 (MQ=255)                                             tctTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTGAACCGAACTGGAAGGCAGCGCCGCAACGCCATGATGGCGCAGGATAAGGGTTTGCTCCGCCAGCTCATCGCGGACGCGGCGAATAGCCCGCGGGCt                                                                             >  2:448924/1‑143 (MQ=255)                                                  tgcttcGGTCTGGGTGGCCTTGCGAGCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                        <  1:895789/141‑1 (MQ=255)                                                  tgctgcGGGCTGGGTGGCCGTGCGATCGTGCCACGGCGTGTTAACCGAACGGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACgg                                                                        <  2:425699/138‑1 (MQ=255)                                                     ttcGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGgaga                                                                     >  1:760501/1‑143 (MQ=255)                                                       gggacggggTGGCCTGGCGATCGTGCCCCGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGaaa                                                                   <  2:460185/137‑1 (MQ=255)                                                         gTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCAAGCACATTGAGGTCGAGGCGAATAGTCTGCGGGCTGACGGAGAAATg                                                                 >  2:855256/1‑143 (MQ=255)                                                                 ggCCGTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCa                                                         <  1:981644/143‑1 (MQ=255)                                                                   ccTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGCCGCGGCGAAGAGTCTGCGGGCTGACCGTGCAATGCTCTACCCGc                                                       >  1:261149/1‑143 (MQ=255)                                                                          aTCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCCCCGCCCTGATCGCGCCGAATCAGCTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCCGCGCACTGACGACCAACTGCTCTACGAGCTCTTCgg                                                >  2:276836/1‑143 (MQ=255)                                                                           tCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGACATGCTCTACGAGCTCTTCggg                                               >  2:632021/1‑142 (MQ=255)                                                                               gCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGCATAGCCTGCGGGCTGACGGCGCAATGCTCTACCAGCGCTTCGGTACtc                                           >  1:241290/1‑142 (MQ=255)                                                                                 cACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTCAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGATCTACCAGCTCTTCGGTACTGAc                                         >  1:494240/1‑143 (MQ=255)                                                                                   cGGCGTGTGGGCCGGGCTGGAAGGCAGCGCCGCACGGCCATGGTGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCGGTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACAt                                       <  2:332910/143‑1 (MQ=255)                                                                                     gCGGGTTATCTGATCTGTATGGCCGCGCCGCACCGGGATGATGGCGCAGGATCAGGTTTTGCTCCGCCTGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTATCAGCTCTTCGGTACTGACATaa                                     <  2:28344/143‑1 (MQ=255)                                                                                         gtTACCCGAACGGGAAGGCAGGGCCGCACCGGCATGATGGCGCAGGAGCAGGTTTTGCGCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCct                                 <  2:711734/143‑1 (MQ=255)                                                                                          ttAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctg                                <  1:285550/143‑1 (MQ=255)                                                                                           tacccGAACGGGTTGGCAGCTCCGCACCGCCATGGTGGCGGAGGATCAGGTTTTGCTCCGCCAGCTCATGGAGGGCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgc                               <  1:482655/140‑1 (MQ=255)                                                                                             aCCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCctgctg                             <  1:855256/143‑1 (MQ=255)                                                                                              ccGAACTGGAGGGCGGGGCCGCACGGCCATGTGGGCGCAGGTTCAGGTTGTGCTCCGCCAGCTCATGGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGt                            <  2:877346/143‑1 (MQ=255)                                                                                                gAACTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGAAGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGttt                          >  2:589322/1‑143 (MQ=255)                                                                                                   cTGGAAGGCAGCGCCGCACCGCCATGATGGCGCAGGATGAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAAc                       <  1:272068/143‑1 (MQ=255)                                                                                                    gggATGGCGGCGCCGGCGCGCCATGGTGGCGCTGGAGCAGGTGTTGCGCCGCCAGCTCATGGCGGTCGCGGCGAATAGTCTGCGGGCCGGCGGCGAAATGCTCTACCAGCTCTTCCGTACTGACATAGCCCTGCTGTTTAccc                      <  2:715571/142‑1 (MQ=255)                                                                                                         ggCAGGGCCGCGCCGCCATGCTGGCGCAGGAGCGGGTGTTGCTCCGCGAGCTCATTGAGGTCGCGGCGAATAGTCTCCGGGCTCACGGAGAAATGCTCTACCAGCTTTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTc                 <  2:21069/143‑1 (MQ=255)                                                                                                          gCAGCGCCGCACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCg                >  2:117986/1‑143 (MQ=255)                                                                                                                  gcACCGCCATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCATGCTGTGTAACCAGTTCGATGAGAcc        >  1:630056/1‑143 (MQ=255)                                                                                                                        ccATGATGGCGCAGGATCAGGTTTTGCTCCGCCAGCTCCTTGAGGTCGCGGCGAATAGTCTGCGGGCTGCCGGAGAAATGCTCTACCAGCTCTTCGGTAGTGACAGAACCATTCTGGATGTCCAGTTCGAGACTACCgctatg  >  2:243124/1‑138 (MQ=255)                                                                                                                                  |                                                                                                                                     TCGAGCCATTGGGGATTTGCTCCGCCACTTTGCGGGCGATGCGCTCTTTTTCTTCGGTCTGGGTGGCCTTGCGATCGTGCCACGGCGTGTTAACCGAACTGGAAGGCAGCGCCGCACCGCCATGATGGC‑CAGGATCAGGTTTTGCTCCGCCAGCTCATTGAGGTCGCGGCGAATAGTCTGCGGGCTGACGGAGAAATGCTCTACCAGCTCTTCGGTACTGACATAACCCTGCTGTTTAACCAGTTCGATAATACCGTTGTG  >  NC_000913/3560327‑3560587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑