New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1805292 =	23 (0.930)	4 (0.160)	4/264	2.7	15.2%	intergenic (+20/+33)	yniD/ydiY	uncharacterized protein/acid‑inducible putative outer membrane protein
	?	NC_000913	1805317 =	22 (0.910)					intergenic (+45/+8)	yniD/ydiY	uncharacterized protein/acid‑inducible putative outer membrane protein
Rejected: Frequency below/above cutoff threshold.

AATGAACATTTTGGACTGAAGGTGGCTTACAACGTCACATGGAACTCTGAACCTCCAGAGTCTGCACCGGAACATACCGACCGTCGGACAACCTTATCGCTGGGCTACAGCATGTAAAATTATCTGCCGGACCGTGTTATGCGGTCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1805441‑1805292 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGT  >  NC_000913/1805317‑1805370                                                                                                                                                                                                              AATGAACATTTTGGACTGAAGGTGGCTTACAACGTCACATGGAACTCTGAACCTCCAGAGTCTGCACCGGAACATACCGACCGTCGGACAACCTTATCGCTGGGCTACAGCATGTAAAATTATCTGCCGGACCGTGTTATGCGGTCTG                                                          <  6:91965/148‑1                CTGAAGGTGGCTTACAACGTCACATGGAACTCTGAACCTCCAGAGTCTGCACCGGAACATACCGACCGTCGGACAACCTTATCGCTGGGCTACAGCATGTAAAATTATCTGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACAT                                          >  4:78865/1‑149                      GTGGCTTACAACGTCACATGGAACTCTGAACCTCCAGAGTCTGCACCGGAACATACCGACCGTCGGACAACCTTATCGCTGGGCTACAGCATGTAAAATTATCAGCCAGACCGCATAACACGGTCCGGCAGATAATTTTACATGCTGTA                                    <  3:78865/149‑1                                                                                                                            CTGCCGGACCGTGTTATGCGGTCTGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGT  <  5:4454/81‑1                                                                                                                            CTGCCGGACCGTGTTATGCGGTCTGGCAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGT  >  6:4454/1‑81                                                                                                                                                                                                              AATGAACATTTTGGACTGAAGGTGGCTTACAACGTCACATGGAACTCTGAACCTCCAGAGTCTGCACCGGAACATACCGACCGTCGGACAACCTTATCGCTGGGCTACAGCATGTAAAATTATCTGCCGGACCGTGTTATGCGGTCTGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1805441‑1805292 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ggcAGATAATTTTACATGCTGTAGCCCAGCGATAAGGTTGTCCGACGGTCGGTATGT  >  NC_000913/1805317‑1805370

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.