mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L001_R1_001.good.fq | 187,777 | 24,421,393 | 100.0% | 130.1 bases | 149 bases | 95.8% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L001_R2_001.good.fq | 187,777 | 24,191,207 | 100.0% | 128.8 bases | 149 bases | 98.0% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L002_R1_001.good.fq | 145,998 | 18,884,732 | 100.0% | 129.3 bases | 149 bases | 95.6% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L002_R2_001.good.fq | 145,998 | 18,712,045 | 100.0% | 128.2 bases | 149 bases | 97.5% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L003_R1_001.good.fq | 168,631 | 21,817,771 | 100.0% | 129.4 bases | 149 bases | 95.6% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L003_R2_001.good.fq | 168,631 | 21,610,133 | 100.0% | 128.2 bases | 149 bases | 97.8% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L004_R1_001.good.fq | 142,705 | 18,414,315 | 100.0% | 129.0 bases | 149 bases | 95.4% |
| errors | Plate-2-BOP-27-SUCB-ALE-3-flask-15_S38_L004_R2_001.good.fq | 142,705 | 18,235,054 | 100.0% | 127.8 bases | 149 bases | 97.3% |
| total | 1,290,222 | 166,286,650 | 100.0% | 128.9 bases | 149 bases | 96.6% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 34.7 | 2.3 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 13696 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 66 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.004 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.88122 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 23:19:15 27 Feb 2020 | 23:19:34 27 Feb 2020 | 19 seconds |
| Read alignment to reference genome | 23:19:35 27 Feb 2020 | 23:21:59 27 Feb 2020 | 2 minutes 24 seconds |
| Preprocessing alignments for candidate junction identification | 23:21:59 27 Feb 2020 | 23:22:16 27 Feb 2020 | 17 seconds |
| Preliminary analysis of coverage distribution | 23:22:16 27 Feb 2020 | 23:22:52 27 Feb 2020 | 36 seconds |
| Identifying junction candidates | 23:22:52 27 Feb 2020 | 23:23:01 27 Feb 2020 | 9 seconds |
| Re-alignment to junction candidates | 23:23:01 27 Feb 2020 | 23:23:46 27 Feb 2020 | 45 seconds |
| Resolving best read alignments | 23:23:46 27 Feb 2020 | 23:24:13 27 Feb 2020 | 27 seconds |
| Creating BAM files | 23:24:13 27 Feb 2020 | 23:24:45 27 Feb 2020 | 32 seconds |
| Tabulating error counts | 23:24:45 27 Feb 2020 | 23:24:57 27 Feb 2020 | 12 seconds |
| Re-calibrating base error rates | 23:24:57 27 Feb 2020 | 23:24:59 27 Feb 2020 | 2 seconds |
| Examining read alignment evidence | 23:24:59 27 Feb 2020 | 23:27:32 27 Feb 2020 | 2 minutes 33 seconds |
| Polymorphism statistics | 23:27:32 27 Feb 2020 | 23:27:33 27 Feb 2020 | 1 second |
| Output | 23:27:33 27 Feb 2020 | 23:27:42 27 Feb 2020 | 9 seconds |
| Total | 8 minutes 26 seconds | ||
