Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	756,830	(G)5→4	coding (924/1767 nt)	sdhA →	succinate dehydrogenase, flavoprotein subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	756,826	0	G	.	100.0%	142.8 / NA	32	coding (920/1767 nt)	sdhA	succinate dehydrogenase, flavoprotein subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (16/16);  total (16/16)

TGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGT  >  NC_000913/756700‑756965                                                                                                                               |                                                                                                                                            tgAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaac                                                                                                                      >  6:136324/1‑149 (MQ=255)   aacaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTc                                                                                                                    >  2:99897/1‑149 (MQ=255)    acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactga                                                                                                                         <  4:47261/143‑1 (MQ=255)    acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactga                                                                                                                         >  3:47261/1‑143 (MQ=255)    acaaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCg                                                                                                                   <  2:96899/149‑1 (MQ=255)     caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa                                                                                                                       >  1:195980/1‑144 (MQ=255)     caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa                                                                                                                       <  2:195980/144‑1 (MQ=255)     caaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGa                                                                                                                  <  5:136324/149‑1 (MQ=255)      aaacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGAt                                                                                                                 <  7:55296/149‑1 (MQ=255)       aacaTGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGGTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATc                                                                                                                <  8:63201/149‑1 (MQ=255)               gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCt                                                                                                            >  1:152635/1‑145 (MQ=255)               gAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCt                                                                                                            <  2:152635/145‑1 (MQ=255)                          ggAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa                                                                                                                       <  6:18052/123‑1 (MQ=255)                          ggAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCgaaactgaaa                                                                                                                       >  5:18052/1‑123 (MQ=255)                            ggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtc                                                                                              <  1:149339/145‑1 (MQ=255)                            ggCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtc                                                                                              >  2:149339/2‑146 (MQ=255)                                             gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc                                                                                            <  8:17768/131‑1 (MQ=255)                                             gCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTtctc                                                                                            >  7:17768/1‑131 (MQ=255)                                               cAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTAt                                                                        >  2:21846/1‑149 (MQ=255)                                                                        gTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCt                                               >  8:8070/1‑149 (MQ=255)                                                                             gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGc                                                                >  2:39298/1‑127 (MQ=255)                                                                             gcgTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGc                                                                <  1:39298/127‑1 (MQ=255)                                                                                    atcatGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCg                                   >  1:195967/1‑149 (MQ=255)                                                                                          aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTccc                                                          >  5:74305/1‑120 (MQ=255)                                                                                          aTCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTccc                                                          <  6:74305/120‑1 (MQ=255)                                                                                                 tCCGTGAAGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGa                      <  1:99897/149‑1 (MQ=255)                                                                                                        aGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                 >  4:108536/1‑131 (MQ=255)                                                                                                        aGGTCGCGGCTGTGATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGt                                 <  3:108536/131‑1 (MQ=255)                                                                                                                  tgtgATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCg                                                 <  6:186999/105‑1 (MQ=255)                                                                                                                    tgATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTg   <  1:21846/149‑1 (MQ=255)                                                                                                                     gATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGt  >  8:161954/1‑149 (MQ=255)                                                                                                                     gATGGTCCGT‑GGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGt  >  1:142504/1‑149 (MQ=255)                                                                                                                               |                                                                                                                                            TGAACAAACATGGCGAACGTTTTATGGAGCGTTATGCGCCGAACGCCAAAGACCTGGCGGGCCGTGACGTGGTTGCGCGTTCCATCATGATCGAAATCCGTGAAGGTCGCGGCTGTGATGGTCCGTGGGGGCCACACGCGAAACTGAAACTCGATCACCTGGGTAAAGAAGTTCTCGAATCCCGTCTGCCGGGTATCCTGGAGCTTTCCCGTACCTTCGCTCACGTCGATCCGGTGAAAGAGCCGATTCCGGTTATCCCAACCTGT  >  NC_000913/756700‑756965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑