Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,632,046	G→T	P164T (CCG→ACG)	guaA ←	GMP synthetase (glutamine aminotransferase)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,632,046	0	G	T	100.0%	94.8 / NA	28	P164T (CCG→ACG)	guaA	GMP synthetase (glutamine aminotransferase)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/14);  total (14/14)

ACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGC  >  NC_000913/2631926‑2632178                                                                                                                         |                                                                                                                                     acTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgat                                                                                                           >  3:73780/1‑148 (MQ=255)          tatCACGCACAATACGCTCCAGTATGCGCATTCCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcggac                                                                                                 <  4:140539/149‑1 (MQ=255)                 cacaAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAg                                                                                          >  5:182517/1‑149 (MQ=255)                  acaAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcggac                                                                                                 <  6:167511/141‑1 (MQ=255)                  acaAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTcggac                                                                                                 >  5:167511/1‑141 (MQ=255)                  acaAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGc                                                                                         >  7:7397/1‑149 (MQ=255)                   caAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCg                                                                                        >  7:38411/1‑149 (MQ=255)                    aaaaCGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCgg                                                                                       <  2:169857/149‑1 (MQ=255)                                         aCCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCa                                                                  >  4:55445/1‑149 (MQ=255)                                             tGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATc                                                                             >  1:70730/1‑134 (MQ=255)                                             tGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATc                                                                             <  2:70730/134‑1 (MQ=255)                                                        aGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGagca                                                   <  6:182517/149‑1 (MQ=255)                                                          tCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgatg                                                                                                          >  2:53465/1‑92 (MQ=255)                                                          tCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGtgatg                                                                                                          <  1:53465/92‑1 (MQ=255)                                                                ccGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGttt                                            <  8:38411/148‑1 (MQ=255)                                                                ccGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTa                                           >  3:101358/1‑149 (MQ=255)                                                                 cGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTa                                           <  4:73780/148‑1 (MQ=255)                                                                  gggTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTAcc                                         <  3:55445/149‑1 (MQ=255)                                                                    gTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGt                                       <  2:6839/149‑1 (MQ=255)                                                                    gTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGt                                       >  1:6839/1‑149 (MQ=255)                                                                     tGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTc                                      <  4:101358/149‑1 (MQ=255)                                                                                gCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTg                                    >  4:50815/1‑140 (MQ=255)                                                                                gCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTg                                    <  3:50815/140‑1 (MQ=255)                                                                                  cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATc                                                        >  7:87943/1‑118 (MQ=255)                                                                                  cATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATc                                                        <  8:87943/118‑1 (MQ=255)                                                                                      gAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCtt                     <  8:7397/149‑1 (MQ=255)                                                                                           gTTTTTCTTCGTTAGCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATa                >  5:165466/1‑149 (MQ=255)                                                                                                         gCCATAATGGCAAACGTGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTATGCAAGACAGCCAGCGCCGTTTTCCCTTATGCGGTCACCGCAAGTTCGATACCGCGAAcactggc  >  1:182200/1‑143 (MQ=255)                                                                                                                         |                                                                                                                                     ACTGGCAGATATCACGCACAAAACGCTCCAGCATGCGCATACCCTGGCGGGTATGAGTCACTTCCGGGTGGAACTGTACGCCATAGAAGCGTTTTTCTTCGTTAGCCATAATGGCAAACGGGCAGCTTTCGGTGCTGGCTACGGTGATGAAGTCGGACGGAATAGCGGTAACTTTATCGCCGTGGCTCATCCAGACATCGAGCAGCGGTTTACCGTCTGCGGTCAGCGCATCTTCGATACCGCGAACCAGTGC  >  NC_000913/2631926‑2632178

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑