Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,100,923	A→G	S116P (TCA→CCA)	gnd ←	6‑phosphogluconate dehydrogenase, decarboxylating

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,100,923	0	A	G	100.0%	330.1 / NA	91	S116P (TCA→CCA)	gnd	6‑phosphogluconate dehydrogenase, decarboxylating
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (48/43);  total (48/43)

AGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGAT  >  NC_000913/2100780‑2101061                                                                                                                                                |                                                                                                                                           aGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCt                                                                                                                                       >  5:326997/1‑149 (MQ=255)      cggcggCGATTTTGGGCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACg                                                                                                                                   <  2:137937/148‑1 (MQ=255)         cggcgATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTa                                                                                                                               <  6:280215/149‑1 (MQ=255)         cggcgATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAACGACCTTTCAGCGCCCCCTCTTCACCGCCACAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTa                                                                                                                               <  6:73997/149‑1 (MQ=255)                 ttGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGaa                                                                                                                        >  6:269045/1‑148 (MQ=255)                    gTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAgt                                                                                                                    >  3:185745/1‑149 (MQ=255)                    gTCAGGATCGGTGCGACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGACCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAACCCCTCTGCTGGAAGCTCACGCTTACGACCAATAgt                                                                                                                    >  6:260532/1‑149 (MQ=255)                      cAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGgaa                                                                                                                                          >  8:70686/1‑125 (MQ=255)                      cAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGgaa                                                                                                                                          <  7:70686/125‑1 (MQ=255)                        ggATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTcc                                                                                                                <  6:326997/149‑1 (MQ=255)                              gTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCATCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTCGAAGCTCACCATTACGACGAATACTGTCCTgg                                                                                                             >  2:7615/1‑146 (MQ=255)                               tGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGgaaga                                                                                                         <  5:269045/149‑1 (MQ=255)                                 cTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag                                                                                                       <  7:389154/149‑1 (MQ=255)                                           aTAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTaccac                                                                                             >  6:260214/1‑149 (MQ=255)                                           aTAGGCTTCTATCTGGCCACCAGGCATAATAGAAGGACCATTCAGCGCCCCCTCTACACCGCCAGAACCACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGATCACCATTACGACGAAAAGAGTCCGGGAAGAAGGTGTAaccac                                                                                             >  6:348012/1‑149 (MQ=255)                                            tAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTaccacc                                                                                            <  4:185745/149‑1 (MQ=255)                                               gCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                   >  3:148951/1‑139 (MQ=255)                                               gCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                   <  4:148951/139‑1 (MQ=255)                                                   ctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                   <  2:395996/135‑1 (MQ=255)                                                   ctttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgtgt                                                                                                   >  1:395996/1‑135 (MQ=255)                                                    tttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTAAGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatga                                                                                    >  5:171991/1‑149 (MQ=255)                                                     ttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   >  7:123678/1‑149 (MQ=255)                                                     ttctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   >  6:203147/1‑149 (MQ=255)                                                      tctGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   <  5:260532/148‑1 (MQ=255)                                                           ccaccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTTCCACCATCAATGATGATGtc                                                                             >  8:388213/1‑149 (MQ=255)                                                             accaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGtctc                                                                           >  7:409607/1‑149 (MQ=255)                                                              ccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgt                                                                                                     <  8:170479/122‑1 (MQ=255)                                                              ccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGgt                                                                                                     >  7:170479/1‑122 (MQ=255)                                                              ccaGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTcc                                                                          >  3:272887/1‑149 (MQ=255)                                                                 ggCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTATGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCttt                                                                       <  6:171991/149‑1 (MQ=255)                                                                  gCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat                                                                                <  7:99617/139‑1 (MQ=255)                                                                  gCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat                                                                                >  8:99617/1‑139 (MQ=255)                                                                    ataataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATc                                                                    >  7:261942/1‑149 (MQ=255)                                                                      aataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   >  3:126710/1‑132 (MQ=255)                                                                      aataGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   <  4:126710/132‑1 (MQ=255)                                                                          gAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCga                                                                  <  6:328462/145‑1 (MQ=255)                                                                          gAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCga                                                                  >  5:328462/1‑145 (MQ=255)                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                  >  6:81079/1‑128 (MQ=255)                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatg                                                                                  <  5:81079/128‑1 (MQ=255)                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   <  6:82342/127‑1 (MQ=255)                                                                           aaGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgat                                                                                   >  5:82342/1‑127 (MQ=255)                                                                              gACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTc                                                                                                                 >  8:343764/1‑94 (MQ=255)                                                                              gACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTc                                                                                                                 <  7:343764/94‑1 (MQ=255)                                                                               aCCTTTCAGCGCCCCCTCTTCACCTCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTTGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGtt                                                         <  5:348012/149‑1 (MQ=255)                                                                                ccTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                        >  7:410527/1‑149 (MQ=255)                                                                                ccTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                        >  6:93507/1‑149 (MQ=255)                                                                                ccTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCAATGAAGTTAAATCCCTCTGCTGGAAGCTCACGATTACTACGAATAGTGTCCTTGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGtt                                                         <  1:139131/148‑1 (MQ=255)                                                                                ccTTTCAGCGCCCCCTCTTCACAGCCAGAAACACCGGTACCAATTAAGTTAAAGCACTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCATGGAAGAAGTTGTTACCACCATCAATTATGATGTATCCTTTATCGAGATATGGtt                                                         >  2:139131/1‑148 (MQ=255)                                                                                 cTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTCACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGttt                                                        >  2:252938/1‑148 (MQ=255)                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTTCCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg                                                     <  7:388213/148‑1 (MQ=255)                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    >  1:377900/1‑149 (MQ=255)                                                                                    tCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAg                                                     <  5:203147/148‑1 (MQ=255)                                                                                     cAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    >  4:193685/1‑148 (MQ=255)                                                                                      aGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGa                                                  <  8:123678/149‑1 (MQ=255)                                                                                       gcgcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGaa                                                 <  5:93507/149‑1 (MQ=255)                                                                                        cgcCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAAt                                                <  4:272887/149‑1 (MQ=255)                                                                                          cccccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCa                                              <  8:261942/149‑1 (MQ=255)                                                                                            cccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCaa                                             >  4:23847/1‑148 (MQ=255)                                                                                            cccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAAt                                            <  5:260214/149‑1 (MQ=255)                                                                                             ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat                                                                                >  3:30648/1‑112 (MQ=255)                                                                                             ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAatgatgat                                                                                <  4:30648/112‑1 (MQ=255)                                                                                             ccTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATa                                           <  3:23847/149‑1 (MQ=255)                                                                                              ctctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTTCGACGAATAGTGTCCTGGATGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                          <  1:7615/149‑1 (MQ=255)                                                                                               tctTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATag                                          >  4:19570/1‑148 (MQ=255)                                                                                                 ttCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag                                                                                                       >  3:250493/1‑85 (MQ=255)                                                                                                 ttCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag                                                                                                       <  4:250493/85‑1 (MQ=255)                                                                                                   cACCGCCAGAAACACCGGTTCCGATGAAGTTTATGCCCTCTTCTGGAAGCTCACGATTTCGACGAATAGTGTCCTGGAAGAAGGTTTTACCACCATCAATGATGATGTCTGCTTTATCGAGATATGGTTTGAGGGAATCATTagca                                        <  4:363039/146‑1 (MQ=255)                                                                                                   cACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATACTGTCCGGGAAGAAGGTCTTACCACCAACAATGAAGATGTCTCATATATCCAGATATGGTTTGAGGGAATCAATaccagc                                      >  5:268843/1‑148 (MQ=255)                                                                                                    aCCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCAt                                    >  2:6096/1‑149 (MQ=255)                                                                                                    aCCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATCTCTCCTTTAACGAGATAACGATTGAGGGAATCAATAGAAGCAt                                    >  2:103671/1‑149 (MQ=255)                                                                                                     ccGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCATTATCTAGATATGGTTTGAGGGAATAAATAGCAGCAta                                   >  6:73116/1‑148 (MQ=255)                                                                                                         cAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTg                               >  1:329094/1‑149 (MQ=255)                                                                                                             aaCACCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTg                           <  8:409607/149‑1 (MQ=255)                                                                                                               cacCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    <  3:259666/122‑1 (MQ=255)                                                                                                               cacCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    >  4:259666/1‑122 (MQ=255)                                                                                                                acCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCaa                                                                                       <  4:151861/86‑1 (MQ=255)                                                                                                                acCGGTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCAc                        <  8:410527/149‑1 (MQ=255)                                                                                                                    gTACCGATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTTTTACCACCATCAATGATGATGTCTCCATTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGc                    <  5:73116/149‑1 (MQ=255)                                                                                                                         gATGAAGTTAAAGCCCTCTGCTGGAAGCTCACGAGTACGACGAATAGTGTCCTGCAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCa               >  2:330057/1‑149 (MQ=255)                                                                                                                          aTGAAGTTATAGCCCTCTGCTGGAAGCTCTCGATTACGACGTTTAGTGTCCTGGAAGAAGGTGTTACCTCCATCATTGATGATGTCTACTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCAGCTGCTTTCAc              <  6:268843/149‑1 (MQ=255)                                                                                                                          aTGAAGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAAGCAATAGCAGCATCCGTGCCTGCACCTGCTTTCAc              >  1:238334/1‑149 (MQ=255)                                                                                                                             aaGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAAGAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGAGGATGGCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCAt           >  7:287677/1‑149 (MQ=255)                                                                                                                              aGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag                                                                                                       >  6:382294/1‑56 (MQ=255)                                                                                                                              aGTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGaagaag                                                                                                       <  5:382294/56‑1 (MQ=255)                                                                                                                               gTTAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCAtt          >  8:55878/1‑148 (MQ=255)                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    >  7:314506/1‑105 (MQ=255)                                                                                                                                ttAAAGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAgg                                                    <  8:314506/105‑1 (MQ=255)                                                                                                                                  aaaGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAAc       <  2:377900/148‑1 (MQ=255)                                                                                                                                  aaaGCCCTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAAc       <  3:193685/148‑1 (MQ=255)                                                                                                                                      cccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGAt  >  2:342448/1‑149 (MQ=255)                                                                                                                                      cccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCAGTGCCTGCACCTGCTTTCACCATTAACAGGAt  >  4:82202/1‑149 (MQ=255)                                                                                                                                       ccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCAc              >  3:38251/1‑136 (MQ=255)                                                                                                                                       ccTCTGCTGGAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCAc              <  4:38251/136‑1 (MQ=255)                                                                                                                                                |                                                                                                                                           AGCTACGGCGGCGATTTTGGTCAGGATCGGTGCTACCAATTCATAGGCTTCTTTCTGGCCACCAGGCATAATAGAAGGACCTTTCAGCGCCCCCTCTTCACCGCCAGAAACACCGGTACCGATGAAGTTAAAGCCCTCTGCTGAAAGCTCACGATTACGACGAATAGTGTCCTGGAAGAAGGTGTTACCACCATCAATGATGATGTCTCCTTTATCGAGATATGGTTTGAGGGAATCAATAGCAGCATCCGTGCCTGCACCTGCTTTCACCATTAACAGGAT  >  NC_000913/2100780‑2101061

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑