mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| read file | reads | bases | passed filters | average | longest | mapped | |
|---|---|---|---|---|---|---|---|
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L001_R1_001.good.fq | 184,033 | 23,311,376 | 100.0% | 126.7 bases | 149 bases | 93.8% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L001_R2_001.good.fq | 184,033 | 22,976,218 | 100.0% | 124.8 bases | 149 bases | 97.2% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L002_R1_001.good.fq | 144,154 | 18,177,289 | 100.0% | 126.1 bases | 149 bases | 93.6% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L002_R2_001.good.fq | 144,154 | 17,907,729 | 100.0% | 124.2 bases | 149 bases | 96.9% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L003_R1_001.good.fq | 164,755 | 20,781,729 | 100.0% | 126.1 bases | 149 bases | 93.6% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L003_R2_001.good.fq | 164,755 | 20,469,193 | 100.0% | 124.2 bases | 149 bases | 97.1% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L004_R1_001.good.fq | 140,749 | 17,721,797 | 100.0% | 125.9 bases | 149 bases | 93.4% |
| errors | Plate-2-BOP-27-PFKA-ALE-4-flask-30_S72_L004_R2_001.good.fq | 140,749 | 17,453,800 | 100.0% | 124.0 bases | 149 bases | 96.7% |
| total | 1,267,382 | 158,799,131 | 100.0% | 125.3 bases | 149 bases | 95.3% |
| seq id | length | fit mean | fit dispersion | % mapped reads | description | ||
|---|---|---|---|---|---|---|---|
| coverage | distribution | NC_000913 | 4,641,652 | 32.7 | 2.5 | 100.0% | Escherichia coli str. K-12 substr. MG1655, complete genome. |
| total | 4,641,652 | 100.0% |
fit dispersion is the ratio of the variance to the mean for the negative binomial fit. It is =1 for Poisson and >1 for over-dispersed data.
| option | limit | actual |
|---|---|---|
| Number of alignment pairs examined for constructing junction candidates | ≤ 100000 | 16058 |
| Coverage evenness (position-hash) score of junction candidates | ≥ 2 | ≥ 3 |
| Test this many junction candidates (n). May be smaller if not enough passed the coverage evenness threshold | 100 ≤ n ≤ 5000 | 119 |
| Total length of all junction candidates (factor times the reference genome length) | ≤ 0.1 | 0.008 |
| reference sequence | pr(no read start) |
|---|---|
| NC_000913 | 0.88394 |
pr(no read start) is the probability that there will not be an aligned read whose first base matches a given position on a given strand.
| option | value |
|---|---|
| Coverage evenness (position-hash) score of predicted junctions must be | ≥ 3 |
| Skew score of predicted junction (−log10 probability of unusual coverage evenness) must be | ≤ 3 |
| Number of bases that at least one read must overlap each uniquely aligned side of a predicted junction | ≥ 1 |
| option | value |
|---|---|
| Mode | Consensus/Mixed Base |
| Ploidy | 1 (haploid) |
| Consensus mutation E-value cutoff | 10 |
| Consensus frequency cutoff | 0.75 |
| Consensus minimum variant coverage each strand | OFF |
| Consensus minimum total coverage each strand | OFF |
| Consensus minimum variant coverage | OFF |
| Consensus minimum total coverage | OFF |
| Polymorphism E-value cutoff | 10 |
| Polymorphism frequency cutoff | 0.2 |
| Polymorphism minimum variant coverage each strand | OFF |
| Polymorphism minimum total coverage each strand | OFF |
| Polymorphism minimum variant coverage | OFF |
| Polymorphism minimum total coverage | OFF |
| Polymorphism bias cutoff | OFF |
| Predict indel polymorphisms | YES |
| Skip indel polymorphisms in homopolymers runs of | OFF |
| Skip base substitutions when they create a homopolymer flanked on each side by | OFF |
| program | version |
|---|---|
| bowtie2 | 2.3.4.1 |
| R | 3.4.4 |
| step | start | end | elapsed |
|---|---|---|---|
| Read and reference sequence file input | 07:45:59 27 Feb 2020 | 07:46:18 27 Feb 2020 | 19 seconds |
| Read alignment to reference genome | 07:46:18 27 Feb 2020 | 07:48:39 27 Feb 2020 | 2 minutes 21 seconds |
| Preprocessing alignments for candidate junction identification | 07:48:39 27 Feb 2020 | 07:48:56 27 Feb 2020 | 17 seconds |
| Preliminary analysis of coverage distribution | 07:48:56 27 Feb 2020 | 07:49:28 27 Feb 2020 | 32 seconds |
| Identifying junction candidates | 07:49:28 27 Feb 2020 | 07:50:16 27 Feb 2020 | 48 seconds |
| Re-alignment to junction candidates | 07:50:16 27 Feb 2020 | 07:51:00 27 Feb 2020 | 44 seconds |
| Resolving best read alignments | 07:51:00 27 Feb 2020 | 07:51:26 27 Feb 2020 | 26 seconds |
| Creating BAM files | 07:51:26 27 Feb 2020 | 07:51:56 27 Feb 2020 | 30 seconds |
| Tabulating error counts | 07:51:56 27 Feb 2020 | 07:52:07 27 Feb 2020 | 11 seconds |
| Re-calibrating base error rates | 07:52:07 27 Feb 2020 | 07:52:10 27 Feb 2020 | 3 seconds |
| Examining read alignment evidence | 07:52:10 27 Feb 2020 | 07:54:32 27 Feb 2020 | 2 minutes 22 seconds |
| Polymorphism statistics | 07:54:32 27 Feb 2020 | 07:54:32 27 Feb 2020 | 0 seconds |
| Output | 07:54:32 27 Feb 2020 | 07:54:47 27 Feb 2020 | 15 seconds |
| Total | 8 minutes 48 seconds | ||
