Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,806,319	C→T	intergenic (‑236/‑51)	ydiY ← / → pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,806,319	0	C	T	95.6%	143.4 / ‑1.8	45	intergenic (‑236/‑51)	ydiY/pfkB	acid‑inducible putative outer membrane protein/6‑phosphofructokinase II
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base T (22/21);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

TTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAC  >  NC_000913/1806177‑1806461                                                                                                                                               |                                                                                                                                               ttATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTc                                                                                                                                          >  2:16154/1‑149 (MQ=255)    taAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCg                                                                                                                                        <  1:16154/148‑1 (MQ=255)       aaaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTACGCTGa                                                                                                                                    >  7:117887/1‑149 (MQ=255)        aaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGAt                                                                                                                                   >  1:57160/1‑149 (MQ=255)        aaGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGAt                                                                                                                                   >  1:57161/1‑149 (MQ=255)         aGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGAtt                                                                                                                                  <  4:59672/149‑1 (MQ=255)             gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                              <  2:57160/149‑1 (MQ=255)             gcgAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGt                                                                                                                              <  2:57161/149‑1 (MQ=255)               gAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGc                                                                                                                            <  8:117887/149‑1 (MQ=255)                          cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACttt                                                                                                                                           >  3:24533/1‑123 (MQ=255)                          cAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACttt                                                                                                                                           <  4:24533/123‑1 (MQ=255)                             tGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAg                                                                                                              >  7:18377/1‑149 (MQ=255)                               tATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAGAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGc                                                                                                             <  6:31765/148‑1 (MQ=255)                                        ttCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                    >  3:21908/1‑148 (MQ=255)                                           aTTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGaa                                                                                                 <  5:100986/148‑1 (MQ=255)                                                 tGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAatgatg                                                                                           <  8:18377/148‑1 (MQ=255)                                                  gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                          >  6:76915/1‑148 (MQ=255)                                                  gAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                          <  5:38659/148‑1 (MQ=255)                                                        gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                    <  7:99841/132‑1 (MQ=255)                                                        gCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATaggag                                                                                                    >  8:99841/1‑132 (MQ=255)                                                              ggCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATAc                                                                             >  6:56529/1‑149 (MQ=255)                                                                        gATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACtt                                                                   <  5:56529/149‑1 (MQ=255)                                                                                    tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGa                                                                                                                        >  5:181214/1‑84 (MQ=255)                                                                                    tatatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGa                                                                                                                        <  6:181214/84‑1 (MQ=255)                                                                                     atatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                          <  5:46082/113‑1 (MQ=255)                                                                                     atatatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATgg                                                                                          >  6:46082/1‑113 (MQ=255)                                                                                        tatTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATa                                                   <  4:21908/149‑1 (MQ=255)                                                                                                tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                         <  6:5359/103‑1 (MQ=255)                                                                                                tGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGt                                                                                         >  5:5359/1‑103 (MQ=255)                                                                                                            aataaaGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCa                                >  6:98503/1‑148 (MQ=255)                                                                                                             ataaaGCTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCaaa                              <  5:98503/149‑1 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATTGTACGTATCTATACGTTGACACTTgc                                                                 >  3:40233/1‑108 (MQ=255)                                                                                                                   cTCCAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTgc                                                                 <  4:40233/108‑1 (MQ=255)                                                                                                                     ccAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                        >  8:14186/1‑99 (MQ=255)                                                                                                                     ccAATAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGa                                                                        <  7:14186/99‑1 (MQ=255)                                                                                                                       aaTAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                             <  4:125055/140‑1 (MQ=255)                                                                                                                       aaTAAATCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAAt                             >  3:125055/1‑140 (MQ=255)                                                                                                                           aaTCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAgg                 >  4:105999/1‑148 (MQ=255)                                                                                                                            aTCATATTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGa                >  7:22583/1‑148 (MQ=255)                                                                                                                                tatTGTTAATTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACt           >  1:89488/1‑149 (MQ=255)                                                                                                                                       aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          <  1:86875/79‑1 (MQ=255)                                                                                                                                       aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGtt                                                                          >  2:86875/1‑79 (MQ=255)                                                                                                                                       aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                   >  6:89816/1‑118 (MQ=255)                                                                                                                                       aaTTTCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTAcccc                                   <  5:89816/118‑1 (MQ=255)                                                                                                                                         tttCTTTACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAc  >  1:175851/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                               TTATAAAAAGGCGCGAATAACTTAGCAATGTATTCTTATTTCATTTTTTGAATAAGCATGTGGCGAAAACAGATTTTTATTTATATATATTTATCTGCAAAATTTTAAATAAAGCTCCAATAAATCATATTGTTAATTTCTTCACTTTCCGCTGATTCGGTGCCAGACTGAAATCAGCCTATAGGAGGAAATGATGGTACGTATCTATACGTTGACACTTGCGCCCTCTCTCGATAGCGCAACAATTACCCCGCAAATTTATCCCGAAGGAAAACTGCGCTGTAC  >  NC_000913/1806177‑1806461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑