Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	555,478	C→A	R290S (CGT→AGT)	cysS →	cysteinyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	555,478	0	C	A	100.0%	117.8 / NA	34	R290S (CGT→AGT)	cysS	cysteinyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (17/17);  total (17/17)

CTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCT  >  NC_000913/555339‑555606                                                                                                                                            |                                                                                                                                 cTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTcc                                                                                                                         >  8:95526/1‑149 (MQ=255) cTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTcc                                                                                                                         >  2:120488/1‑149 (MQ=255)        cATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTc                                                                                                                  >  3:119005/1‑149 (MQ=255)            atgGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGc                                                                                                              <  7:95526/149‑1 (MQ=255)             tgGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGc                                                                                                              >  1:189056/1‑148 (MQ=255)                    tATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCg                                                                                                      <  4:119005/149‑1 (MQ=255)                        tgAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGc                                                                                                  >  6:10621/1‑149 (MQ=255)                        tgAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGc                                                                                                  <  5:10621/149‑1 (MQ=255)                          aaCTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATc                                                                                                       >  3:25010/1‑142 (MQ=255)                          aaCTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATc                                                                                                       <  4:25010/142‑1 (MQ=255)                                         tCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCg                                                                                  >  7:94086/1‑148 (MQ=255)                                           ggggATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGaa                                                                                <  2:189056/148‑1 (MQ=255)                                                   tgatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTg                                                                       <  1:120488/149‑1 (MQ=255)                                                    gatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTg                                                                       >  4:46734/1‑148 (MQ=255)                                                    gatggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTg                                                                       >  6:76624/1‑148 (MQ=255)                                                     atggtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGaa                                                                     <  5:76624/149‑1 (MQ=255)                                                        gtTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAg                                                                    >  4:148224/1‑147 (MQ=255)                                                                       aTGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCt                                                   <  3:46734/149‑1 (MQ=255)                                                                            cAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGc                                              <  8:94086/149‑1 (MQ=255)                                                                                    tGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGaa                                                                                >  5:174546/1‑107 (MQ=255)                                                                                    tGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGaa                                                                                <  6:174546/107‑1 (MQ=255)                                                                                                 tACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACtgcgctgcgc                          <  3:148224/148‑1 (MQ=255)                                                                                                   ccGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGa                                                                      >  3:54005/1‑102 (MQ=255)                                                                                                   ccGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGa                                                                      <  4:54005/102‑1 (MQ=255)                                                                                                         gcgATGTGCTGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGa                  >  1:50382/1‑148 (MQ=255)                                                                                                                  tGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAg                   >  5:49275/1‑138 (MQ=255)                                                                                                                  tGAAATACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAg                   <  6:49275/138‑1 (MQ=255)                                                                                                                      aTACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGaa                                                                           <  3:46046/78‑1 (MQ=255)                                                                                                                      aTACTACGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGaa                                                                           >  4:46046/1‑78 (MQ=255)                                                                                                                         ctacGACGCGGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCt  <  2:50382/148‑1 (MQ=255)                                                                                                                                gcgGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGcgcg                         >  6:39697/1‑118 (MQ=255)                                                                                                                                gcgGAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGcgcg                         <  5:39697/118‑1 (MQ=255)                                                                                                                                  ggAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgcg     >  7:68884/1‑136 (MQ=255)                                                                                                                                  ggAAACCGTGAGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTgcg     <  8:68884/136‑1 (MQ=255)                                                                                                                                            |                                                                                                                                 CTGTGCCCATGATGGTCAGTATGTGAACTACTGGATGCACTCGGGGATGGTGATGGTTGACCGCGAGAAGATGTCCAAATCGCTGGGTAACTTCTTTACCGTGCGCGATGTGCTGAAATACTACGACGCGGAAACCGTGCGTTACTTCCTGATGTCGGGCCACTATCGCAGCCAGTTGAACTACAGCGAAGAGAACCTGAAGCAGGCGCGTGCGGCGCTGGAGCGTCTCTACACTGCGCTGCGCGGCACAGATAAAACCGTTGCGCCT  >  NC_000913/555339‑555606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑