Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,662,350 C→T P599S (CCC→TCC)  ynfF → S‑ and N‑oxide reductase, A subunit, periplasmic

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,662,3500CT100.0% 81.2 / NA 25P599S (CCC→TCC) ynfFS‑ and N‑oxide reductase, A subunit, periplasmic
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (13/12);  total (13/12)

AAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAA  >  NC_000913/1662212‑1662493
                                                                                                                                          |                                                                                                                                               
aaaCCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTAc                                                                                                                                         >  8:95461/1‑147 (MQ=255)
      aTCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGAt                                                                                                                                 >  5:90022/1‑149 (MQ=255)
              gATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAAc                                                                                                                           <  5:65212/147‑1 (MQ=255)
                 gCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCg                                                                                                                      >  4:44236/1‑149 (MQ=255)
                                    cGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATg                                                                                                   <  6:90022/149‑1 (MQ=255)
                                     gCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGc                                                                                                  <  7:95461/149‑1 (MQ=255)
                                         aGGACCAGCCGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACTACCGGGATCTTTAAGAAAAAATGCCCg                                                                                               >  1:70093/1‑148 (MQ=255)
                                                      tATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACg                                                                                  <  3:44236/148‑1 (MQ=255)
                                                                               gacAGCATGAAAGGATCAAATAACTCCATGAGAAAACGAAGGAACGTAACCCTCAGATCTCCGACTACGAGGAGATGAAAACGACCGCCAACTTTAAGAAAAAATccc                                                                                                 >  3:61873/3‑108 (MQ=255)
                                                                               gTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGAt                                                        <  1:190145/149‑1 (MQ=255)
                                                                                tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACt                                                                                                                                           >  5:105096/1‑65 (MQ=255)
                                                                                tCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACt                                                                                                                                           <  6:105096/65‑1 (MQ=255)
                                                                                     aTGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAg                                                  >  1:23816/1‑149 (MQ=255)
                                                                                            gATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAAcc                                           <  2:23816/149‑1 (MQ=255)
                                                                                                aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaa                                                                                                      <  5:10785/86‑1 (MQ=255)
                                                                                                aaaTATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGaaaaa                                                                                                      >  6:10785/1‑86 (MQ=255)
                                                                                                         cttGCGAAAACGATGGAACGTAACCCTGAGATGTCCCATTACGCGTTTATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGaaaa                                  <  2:70093/143‑1 (MQ=255)
                                                                                                            gCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                                         <  1:158740/135‑1 (MQ=255)
                                                                                                            gCGAAAACGAAGGAACGTAACCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCg                                         >  2:158740/1‑135 (MQ=255)
                                                                                                                              aaCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                         >  5:159827/1‑133 (MQ=255)
                                                                                                                              aaCCCTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCgg                         <  6:159827/133‑1 (MQ=255)
                                                                                                                                 ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                             >  1:87449/1‑110 (MQ=255)
                                                                                                                                 ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCaaa                                             <  2:87449/110‑1 (MQ=255)
                                                                                                                                 ccTGAGATGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTc      >  3:47732/1‑149 (MQ=255)
                                                                                                                                     agaTGTCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGaa  >  4:7288/1‑149 (MQ=255)
                                                                                                                                          |                                                                                                                                               
AAACCCATCTACTGGATGCTGAGTGAAGTCGCCAAACGCTTAGGACCAGACGTTTATCAAACCTTTACTGAAGGTCGCAGTCAGCATGAATGGATCAAATATCTCCATGCGAAAACGAAGGAACGTAACCCTGAGATGCCCGACTACGAGGAGATGAAAACGACCGGGATCTTTAAGAAAAAATGCCCGGAAGAACACTACGTCGCTTTCCGCGCATTCCGTGAAGATCCACAGGCAAACCCGTTGAAAACACCTTCGGGGAAAATCGAAATTTATTCTGAA  >  NC_000913/1662212‑1662493

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑