Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 238,653 C→A L28F (TTG→TTT ykfM ← lethality reduction protein, putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913238,6530CA93.7% 262.5 / 9.9 79coding (84/480 nt)ykfMlethality reduction protein, putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (31/43);  minor base . (3/2);  total (34/45)
Fisher's exact test for biased strand distribution p-value = 6.47e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01

ATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTC  >  NC_000913/238508‑238796
                                                                                                                                                  |                                                                                                                                               
aTTTTATTATAGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACATAATCAACATTCTCAAATCATAAAATATTTCCGTGGAGCATTTTATTATTTAATATAGAGGTTTAACTCCGGTAaaaaaaaa                                                                                                                                               <  4:155282/148‑1 (MQ=255)
 ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAAGCGCAGCACAGAATCACCATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGCGGTTGACCTCCGGTAaaaaaaaat                                                                                                                                              >  2:85853/1‑147 (MQ=255)
 ttttATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaa                                                                                                                                              >  4:99010/1‑148 (MQ=255)
 ttttATTATAGTAACTAAAATTACATTAATATTTAAAAAGGAAAACTACATGAAAACGAATCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaag                                                                                                                                             <  5:179121/149‑1 (MQ=255)
   ttattaTCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGTACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaaga                                                                                                                                            <  4:69886/148‑1 (MQ=255)
     attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaagaag                                                                                                                                          <  3:6981/148‑1 (MQ=255)
     attatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCa                                                                                                                                        >  8:80692/1‑149 (MQ=255)
      ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACTACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGc                                                                                                                                         <  3:123006/148‑1 (MQ=255)
      ttatCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTaaaaaaaaaaagaag                                                                                                                                         <  7:180964/148‑6 (MQ=255)
        atCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaagaa                                                                                                                                           <  1:8150/144‑1 (MQ=255)
        atCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                      >  7:316123/1‑149 (MQ=255)
        atCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATAAAGAGGATTAACTCCGATAAAAAAAAaacaaaaaa                                                                                                                                       >  2:8150/1‑144 (MQ=255)
         tCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCAtt                                                                                                                                      >  1:232313/1‑148 (MQ=255)
           gtaagtaaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTACGTTAAAAAAAAAAGAAGCATAGaa                                                                                                                                   >  2:63530/1‑149 (MQ=255)
                taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAg                                                                                                                              <  5:283253/149‑1 (MQ=255)
                taaAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAg                                                                                                                              <  2:282918/149‑1 (MQ=255)
                 aaaaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAg                                                                                                                              <  4:201016/148‑1 (MQ=255)
                   aaTTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAggg                                                                                                                            >  5:195297/1‑148 (MQ=255)
                           gAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAataata                                                                                                                  >  6:261066/1‑149 (MQ=255)
                            aataatTAAAAAGGAAAACGACATGAAACCGAACCACACAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATATAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAaataat                                                                                                                   <  1:185591/148‑1 (MQ=255)
                             atatTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAatat                                                                                                                 <  1:82029/149‑1 (MQ=255)
                                tttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATgg                                                                                                               >  5:200034/1‑148 (MQ=255)
                                tttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGAATTGAAGGCAGGGAAAAATAATTTgg                                                                                                               >  6:184197/1‑148 (MQ=255)
                                tttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCAGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCAGGTAAAAAAAAAAGAAGAATTGAATTAAGGGAAAAATAATATgg                                                                                                               >  3:188863/1‑148 (MQ=255)
                                 ttAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGc                                                                                                              >  2:243636/1‑148 (MQ=255)
                                    aaaaGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGAATTGAATGAAGGGAAAAATAAAATGGCAAt                                                                                                           >  3:209596/1‑148 (MQ=255)
                                        ggAAAACGACATTAAACCGAAGCACAGAATTAACATTCTCCAATCATAAAAAATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCAGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATaaaaa                                                                                                      <  4:111509/149‑1 (MQ=255)
                                            aaCGACATGAAACCTAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACAt                                                                                                  <  8:301849/149‑1 (MQ=255)
                                             aCGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATc                                                                                                 <  3:138811/149‑1 (MQ=255)
                                                 cATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCgaaa                                                                                             <  1:241366/149‑1 (MQ=255)
                                                        ccGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAActc                                                                                      <  1:93688/149‑1 (MQ=255)
                                                          gAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCtt                                                                                    <  5:333407/149‑1 (MQ=255)
                                                           aaGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTTAATATAGAGTTTTTACTCCTGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCttt                                                                                   <  5:155857/149‑1 (MQ=255)
                                                               acaGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaat                                                                               >  8:11021/1‑149 (MQ=255)
                                                               acaGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGAATTGAATGAGGGTAAAAAAAATTTGGCAAAAAAAAACATCGAAAGAAACTATtttaat                                                                               >  2:46102/1‑149 (MQ=255)
                                                                   aaTCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTAATATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCTTAAAAATCATCGAAAGAAACTCTtttaatttaa                                                                           <  6:62255/149‑1 (MQ=255)
                                                                    ataaaCAATCTACAATCATAAAATATTTCCGTTGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTtttaatttaa                                                                           <  4:188863/145‑1 (MQ=255)
                                                                    aTCAACATTATCCAATCATAAAATTTTTCCGTGGAGCATTTTATTATTTAATATAGAGGTTTAACTACGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAAc                                                                          <  4:209596/149‑1 (MQ=255)
                                                                     tCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACAt                                                                                                  <  4:130201/124‑1 (MQ=255)
                                                                     tCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACAt                                                                                                  >  3:130201/1‑124 (MQ=255)
                                                                       aaCATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATACAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAATAAACTCATTAAATTTAAc                                                                          >  4:121865/1‑146 (MQ=255)
                                                                           ttCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTaaa                                                                   <  6:200034/149‑1 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATTCAGGGAAAAATAATATGGCCATAAAAAACAAAGAAAAAAACTCTTTTAATTTAACATGTAAACGCAt                                                              >  8:26315/1‑149 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                              <  8:86795/149‑1 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                              >  1:46049/1‑149 (MQ=255)
                                                                                cAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                              <  2:232313/149‑1 (MQ=255)
                                                                                cAATCATAAAATAATTCCGTTGATCATTTTATTATTTAATTTAGATGTTTATATCCGGTTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATTATATGTCCATAAAAAACATCGTAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                              <  2:154121/149‑1 (MQ=255)
                                                                                aaaTCATAAAATATTGCCCGCGAGAATTTTTTTATTTCATATATTTTTTTAACTGCGGTAAAAAAAAAAGAAGCATTGACGTCAGGTAAAAATAATATGGCCATAAACAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCAt                                                              <  6:240769/148‑1 (MQ=255)
                                                                                  aTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaag                                                                                                                                             <  5:143874/68‑1 (MQ=255)
                                                                                  aTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAaag                                                                                                                                             >  6:143874/1‑68 (MQ=255)
                                                                                  aTCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATgg                                                            >  1:195122/1‑149 (MQ=255)
                                                                                   tCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGt                                                           >  8:190970/1‑148 (MQ=255)
                                                                                      tAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTaa                                                        >  7:39118/1‑149 (MQ=255)
                                                                                        aaaTATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATc                                                      >  7:173453/1‑149 (MQ=255)
                                                                                             ttttCGTTGATCATTTTATTATTTAAAATATACGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCat                                                 <  1:46102/149‑1 (MQ=255)
                                                                                                  gTGGATCAATTTATTATTTAATATATTTGTTTAAAACCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCAc                                            <  5:184197/149‑1 (MQ=255)
                                                                                                     gAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACggg                                         <  1:265707/149‑1 (MQ=255)
                                                                                                      aGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGt                                        >  8:277159/1‑149 (MQ=255)
                                                                                                      aGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGt                                        >  8:115063/1‑149 (MQ=255)
                                                                                                       gCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTg                                       >  2:234659/1‑149 (MQ=255)
                                                                                                        cATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGa                                     <  7:190970/149‑1 (MQ=255)
                                                                                                         attttattttTTAATATACTTGTTTAAATTCGGTAAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTg                                       <  4:258009/148‑1 (MQ=255)
                                                                                                              attattGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAgtgt                                 <  2:46049/148‑1 (MQ=255)
                                                                                                                tattGAATATAGCGGGTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTaa                              <  4:249486/149‑1 (MQ=255)
                                                                                                                tattGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGtt                                >  5:281645/1‑147 (MQ=255)
                                                                                                                 attGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTg                                       <  7:88110/139‑1 (MQ=255)
                                                                                                                 attGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTg                                       >  8:88110/1‑139 (MQ=255)
                                                                                                                 attGAATATAGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGtt                                >  6:257073/1‑146 (MQ=255)
                                                                                                                      atatatAGTTTTAAATCCGTTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacat                        <  7:26315/149‑1 (MQ=255)
                                                                                                                       tataGAGGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacata                       <  6:281645/149‑1 (MQ=255)
                                                                                                                          agatGTTTAAATCATGTAAAAAAAAAAGAAGCATTGAATGCAGTGAAAAATAATATGTCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacataca                     <  1:63530/148‑1 (MQ=255)
                                                                                                                          agagGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacataca                     >  5:208753/1‑148 (MQ=255)
                                                                                                                            agGTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATaa                  <  1:234659/149‑1 (MQ=255)
                                                                                                                              gTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacat                        <  4:65323/141‑1 (MQ=255)
                                                                                                                              gTTTAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAacat                        >  3:65323/1‑141 (MQ=255)
                                                                                                                               ttttACTCCGGTTAAATAATAAGAAGCTTTGAATGCAGTGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATg               <  3:121865/149‑1 (MQ=255)
                                                                                                                               tttAACTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAAt                <  7:11021/148‑1 (MQ=255)
                                                                                                                                ttAACTCCGGTAAAAAAAAAAGAAGCATTGATTGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATg               >  1:26138/1‑148 (MQ=255)
                                                                                                                                  aaCTCCGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGa             <  8:39118/148‑1 (MQ=255)
                                                                                                                                   aCTCCGGTAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTc          <  7:80692/149‑1 (MQ=255)
                                                                                                                                       cGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCAt        <  2:195122/148‑1 (MQ=255)
                                                                                                                                       cGGTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATg       >  7:98311/1‑149 (MQ=255)
                                                                                                                                         gTAAAAAAAAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTATTTTAATTTAACATTTAAACGCATGGTTAATCCTCATATAACGGGTGGAGTGTTAAGAACATACATAAATTGAGTAATGtt     >  4:85743/1‑149 (MQ=255)
                                                                                                                                             aaaaaaaaAGAAGCATTGAATGCAGGGTAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATCTAAACGCATGGTAAATGCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTc  <  1:13759/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
ATTTTATTATCGTAAGTAAAATTACATGAATATTTAAAAAGGAAAACGACATGAAACCGAAGCACAGAATCAACATTCTCCAATCATAAAATATTTCCGTGGAGCATTTTATTATTGAATATAGAGGTTTAACTCCGGTAAAAAACAAAGAAGCATTGAATGCAGGGAAAAATAATATGGCCATAAAAAACATCGAAAGAAACTCTTTTAATTTAACATGTAAACGCATGGTTAATCCTCATATCACGGGTGGAGTGTTAAGAACATACATAAATGGAGTCATGTTTTC  >  NC_000913/238508‑238796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: