Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,149,615	G→T	D457E (GAC→GAA)	yegI ←	protein kinase‑related putative non‑specific DNA‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,149,615	0	G	T	100.0%	111.8 / NA	32	D457E (GAC→GAA)	yegI	protein kinase‑related putative non‑specific DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/18);  total (14/18)

CCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGGTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGC  >  NC_000913/2149471‑2149760                                                                                                                                                 |                                                                                                                                                  ccTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCa                                                                                                                                                >  7:82464/1‑148 (MQ=255)     gCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGt                                                                                                                                            <  5:115217/148‑1 (MQ=255)              tGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGc                                                                                                                                  <  2:72062/149‑1 (MQ=255)                      tgcCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTa                                                                                                                           >  1:32084/1‑148 (MQ=255)                        ccAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCa                                                                                                                                                >  4:32012/1‑125 (MQ=255)                        ccAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCa                                                                                                                                                <  3:32012/125‑1 (MQ=255)                        ccAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTaaa                                                                                                                         <  7:8961/148‑1 (MQ=255)                        ccAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAAt                                                                                                                        <  8:82464/149‑1 (MQ=255)                                                   aCCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGc                                                                                              <  5:94076/148‑1 (MQ=255)                                                     ccGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCt                                                                                                        <  5:120263/136‑1 (MQ=255)                                                     ccGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCt                                                                                                        >  6:120263/1‑136 (MQ=255)                                                             ttCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCtt                                                                                   >  2:28697/1‑149 (MQ=255)                                                                   cTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTg                                                                             >  7:163481/1‑149 (MQ=255)                                                                    ttCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGa                                                                                                      <  8:7507/123‑1 (MQ=255)                                                                    ttCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGa                                                                                                      >  7:7507/1‑123 (MQ=255)                                                                            ttCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGc                                                                                                         <  6:28001/112‑1 (MQ=255)                                                                              cGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGcc                                                                   <  8:163481/148‑1 (MQ=255)                                                                                    tCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTg                                                                                                       <  1:132199/106‑1 (MQ=255)                                                                                    tCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTg                                                                                                       >  2:132199/1‑106 (MQ=255)                                                                                     cGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATc                                                            >  7:20652/1‑148 (MQ=255)                                                                                          gTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGa                                                        >  3:31169/1‑147 (MQ=255)                                                                                              ggCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAgccg                                                  >  5:5155/1‑149 (MQ=255)                                                                                               gCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAgccgc                                                 >  1:124582/1‑149 (MQ=255)                                                                                                       aCCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAg                                         <  4:31169/149‑1 (MQ=255)                                                                                                             cAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAAcc                                    <  2:32084/148‑1 (MQ=255)                                                                                                              aGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTc                                                                           >  2:166481/1‑108 (MQ=255)                                                                                                              aGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTc                                                                           <  1:166481/108‑1 (MQ=255)                                                                                                                  cgccTACCTGCTGGATCTGGCGGACTAAATGTTCa                                                                                                                                                >  4:34027/1‑35 (MQ=255)                                                                                                                  cgccTACCTGCTGGATCTGGCGGACTAAATGTTCa                                                                                                                                                <  3:34027/35‑1 (MQ=255)                                                                                                                               gATCTGGCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCgg                   <  3:17135/147‑1 (MQ=255)                                                                                                                                     gCGGACTAAATGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGcc            <  6:5155/148‑1 (MQ=255)                                                                                                                                              aTGTTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGc  <  8:20652/149‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                  CCTTGCAGTGTCATGAAGTGCTGCCAGAGCGCGTTTCTCTTCTTCCGGTAACCCGAGAATTTCGTCCTTCATTTTTTCGAGCATCGTCCGTTTGGCGATAAAACCTTCCAGGCCGCCTACCTGCTGGATCTGGCGGACTAAATGGTCATAGTCCATTTTCGCACGATTAAATGCCTCACGGCGTTGCTGAACTTCTGCTTTGTACGCTTTGCTTGTCAGACTGCCAATAATCCAGATAGCCGCCAGCACAGGAACCAAAATAATATAACGCGGTTCTGCCTGAAGGCCGC  >  NC_000913/2149471‑2149760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑