Predicted mutation | ||||||
---|---|---|---|---|---|---|
evidence | seq id | position | mutation | annotation | gene | description |
RA | NC_000913 | 3,964,929 | C→T | E234E (GAG→GAA) | rhlB ← | ATP‑dependent RNA helicase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,964,929 | 0 | C | T | 99.2% | 420.3 / ‑6.5 | 121 | E234E (GAG→GAA) | rhlB | ATP‑dependent RNA helicase |
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (57/63); total (57/64) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
Only 100 of 125 total aligned reads displayed.
CACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCA > NC_000913/3964784‑3965074 | cacGATGACCATCTGCTGCCAGGTCGCCCCAGATCTCTTCACCACGGTCTTAGGAGCTGGCGAAAATACTCCCTCTGCCTGGCCACTCTTCTTCGCTCCGCGGTTGCAGCAAACGCATTTTTGCCTCGATAG‑AAGGGTAGACAAGttct < 2:149899/149‑1 (MQ=255) acGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTtctt > 2:250794/1‑149 (MQ=255) cGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGttcttc < 1:171853/149‑1 (MQ=255) gATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTtcttct > 3:213904/1‑149 (MQ=255) gATGACCATCTGCTGCCAGGTGGCCCAACATCTCTTCAGAACGGTGTTTGGTGTTGGCGATAATAATCGATCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTtcttct < 7:364583/149‑1 (MQ=255) aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTcttctt < 4:10240/149‑1 (MQ=255) aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTcttctt < 7:66820/149‑1 (MQ=255) aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTcttctt < 6:168141/149‑1 (MQ=255) aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTcttctt < 4:153735/149‑1 (MQ=255) aTGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTcttctt < 5:359320/149‑1 (MQ=255) tGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCttt < 2:313374/149‑1 (MQ=255) cATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATa > 7:320572/1‑149 (MQ=255) cATCTGCTGCCAGGTGGCCCCAGATATCTTCACAACGGGGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCTTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATa > 5:118686/1‑149 (MQ=255) aTCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATAc > 6:390003/1‑149 (MQ=255) tgctgcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGt > 6:394958/1‑149 (MQ=255) ctgcCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTg < 1:146079/148‑1 (MQ=255) ctgcCAGGTGGCACCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTgg > 8:389018/1‑149 (MQ=255) ccAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGTCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTATGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGAGGacc > 5:39771/1‑149 (MQ=255) ccAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACTGTGGccc > 4:311953/1‑149 (MQ=255) ccAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGccc > 1:179973/1‑149 (MQ=255) aGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGt > 4:160059/1‑149 (MQ=255) gTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGttt > 8:213634/1‑149 (MQ=255) ggCCACAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTc > 2:27062/1‑149 (MQ=255) gCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCt < 2:370534/149‑1 (MQ=255) ccccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTg < 3:334067/149‑1 (MQ=255) cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGttcttc < 8:179030/124‑1 (MQ=255) cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGttcttc > 7:179030/1‑124 (MQ=255) cccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGt > 7:82182/1‑149 (MQ=255) ccAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGtt > 1:30464/1‑149 (MQ=255) aGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTcc > 4:360854/1‑149 (MQ=255) gATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCg < 7:130264/149‑1 (MQ=255) gATCTCTTCACAACGGTGTTTGGGGATGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCg < 2:130986/149‑1 (MQ=255) aTCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTc > 6:229254/1‑141 (MQ=255) ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATGCGGTGGCCCGTTTTCt < 2:176150/137‑1 (MQ=255) ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATGCGGTGGCCCGTTTTCt > 1:176150/1‑137 (MQ=255) ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGTCCCGTTTTctgttcctgttcaa > 7:308008/1‑147 (MQ=255) ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGt < 4:213904/145‑1 (MQ=255) ttCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCa > 8:237289/1‑149 (MQ=255) acaACGTTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt > 4:236045/1‑149 (MQ=255) acaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt > 1:185309/1‑149 (MQ=255) acaACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGAACAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt > 6:122818/1‑149 (MQ=255) aCGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTa > 6:13602/1‑112 (MQ=255) aCGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTa < 5:13602/112‑1 (MQ=255) aCGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGAACAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCaa < 5:122818/149‑1 (MQ=255) gttGGTGGTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGGTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATAtt < 1:172805/148‑1 (MQ=255) tGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTc < 7:213634/148‑1 (MQ=255) ggTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGtttt > 4:37597/1‑120 (MQ=255) ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt < 6:291105/133‑1 (MQ=255) ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACtt > 5:291105/1‑133 (MQ=255) ttGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCAtt < 7:237289/149‑1 (MQ=255) gAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTcc > 5:43411/1‑148 (MQ=255) ataatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTg < 4:90466/149‑1 (MQ=255) aatCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAGAAAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTc < 5:188473/118‑1 (MQ=255) atCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCt < 2:179973/148‑1 (MQ=255) atCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc > 7:312885/1‑149 (MQ=255) tCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCg < 1:287795/149‑1 (MQ=255) tCGCTCTGTCTCGCCACTCTTCATCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGc > 8:397238/1‑146 (MQ=255) ctctGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAAc < 1:27062/149‑1 (MQ=255) tgtctgGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACg < 2:185309/147‑1 (MQ=255) ggCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGtt > 1:39002/1‑148 (MQ=255) gCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCa < 7:181821/149‑1 (MQ=255) gCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCAGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGAATTGTTCATCTGCTCGAACGCCAGTTCa > 5:288032/1‑149 (MQ=255) ccACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc < 8:320572/149‑1 (MQ=255) ccACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc < 6:58653/149‑1 (MQ=255) ccACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCAc < 7:389018/149‑1 (MQ=255) cACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACg < 5:394958/149‑1 (MQ=255) ctctTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTa > 6:319428/1‑149 (MQ=255) cttcttCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTc > 1:167212/1‑112 (MQ=255) ttcttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAccc > 3:141566/1‑149 (MQ=255) cttcGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTAccc < 7:112440/147‑1 (MQ=255) gttgaGCGTTTGCTGCAAACGCATTTTTTCATCGTTAG‑AAGGGTTGAAATGTTCTTGTTTAATTTGGTGGCCCGTTTTCTGTTCCGGTTGCACTGCTTTATTTTGGGGATTGTTCATGTGCTCGATCGGGAGTTCAGGTACCCGGTAc < 2:387099/144‑1 (MQ=255) gATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTAc > 4:366523/1‑148 (MQ=255) aTCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc > 4:66177/1‑122 (MQ=255) aTCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTc < 3:66177/122‑1 (MQ=255) aTCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTGCATCTGCTCGAACGCCAGTGCACGTACCCGGTACg > 2:40055/1‑148 (MQ=255) tCAGCGTTTGCCGCAAACGCATTTTTTCTTCGTTCG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGGTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCCCGTACCCGGGACgc > 6:65696/1‑147 (MQ=255) aGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGaaa < 3:360854/148‑1 (MQ=255) gCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGc < 7:349808/149‑1 (MQ=255) tttGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTc > 2:178177/1‑97 (MQ=255) tttGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTc < 1:178177/97‑1 (MQ=255) tttGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTg < 1:250794/149‑1 (MQ=255) cagcaAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCg < 1:50530/148‑1 (MQ=255) agcaAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCg > 2:205401/1‑147 (MQ=255) agcaAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGga < 8:312885/149‑1 (MQ=255) aaCGCATTTTTGCTTCCTTAG‑AAGGGTAGAAAAGTTCTTCTATAATACGGTGGCCCGTATTCTGTTCCGGTTCCACTTCAATATAATCGGCATTGTTCATCTGCTCGAAAGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAAc < 6:171905/149‑1 (MQ=255) cGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAg < 5:390003/149‑1 (MQ=255) aTTTTTTCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCAt > 5:290684/1‑149 (MQ=255) ttttttCTTCGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTAACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATg > 6:2610/1‑149 (MQ=255) tcttcGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGa < 2:70576/148‑1 (MQ=255) tcttcGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAg > 7:40355/1‑149 (MQ=255) cGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgcg < 2:39002/148‑1 (MQ=255) cGTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGgc < 3:366523/147‑1 (MQ=255) gTTAG‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCt > 3:128126/1‑149 (MQ=255) g‑AAGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGt < 8:40355/148‑1 (MQ=255) aaGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACTAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGttt < 3:160059/149‑1 (MQ=255) aGGGTAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGTATGGCGGAGAACAGCATGTTGAGGCGCTGGTTTg < 4:149272/149‑1 (MQ=255) tAGAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAgg < 3:74489/149‑1 (MQ=255) gAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAggcg < 3:236045/149‑1 (MQ=255) gAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAggcg > 8:354623/1‑149 (MQ=255) gAAAAGTTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAggcg > 3:256594/1‑149 (MQ=255) | CACGATGACCATCTGCTGCCAGGTGGCCCCAGATCTCTTCACAACGGTGTTTGGTGTTGGCGAAAATAATCGCTCTGTCTGGCCACTCTTCTTCGATCAGCGTTTGCAGCAAACGCATTTTTTCTTCGTTAG‑AAGGGTAGAAAAGCTCTTCTTTAATACGGTGGCCCGTTTTCTGTTCCGGTTCCACTTCAATATATTCGGCATTGTTCATCTGCTCGAACGCCAGTTCACGTACCCGGTACGAAAGCGTGGCGGAGAACAGCATGTTGAGGCGCTGGTTTGCAGGCGGCA > NC_000913/3964784‑3965074 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |