Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913753,6580CA68.5% 80.0 / 68.5 74A271S (GCG→TCG) gltAcitrate synthase
Reads supporting (aligned to +/- strand):  ref base C (14/9);  new base A (26/24);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 6.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.30e-01
Rejected as consensus: Frequency below/above cutoff threshold.

CATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATA  >  NC_000913/753512‑753803
                                                                                                                                                  |                                                                                                                                                 
cATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCtt                                                                                                                                                 <  7:214680/149‑1 (MQ=255)
 aTTACGGTGGCGAGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAATAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTc                                                                                                                                                <  1:123865/149‑1 (MQ=255)
     cGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGt                                                                                                                                              >  1:393924/1‑147 (MQ=255)
            gcgcgGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCAccgc                                                                                                                                     >  6:53278/1‑149 (MQ=255)
                     gTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAg                                                                                                                            >  8:393296/1‑149 (MQ=255)
                         tttttGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTcc                                                                                                                        <  5:53278/149‑1 (MQ=255)
                          ttttGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTccc                                                                                                                       >  2:279319/1‑149 (MQ=255)
                                acacGCGTTGACCGAAGCCCATCAGGCGGAAACAATCATTTATGTCTTTAGCACGACTAACAAATTCCAGAATGTGATTAAAGCATCTGAATTCTTCCAGCATTTTAAGAGCCGCTTCGtt                                                                                                                                             >  8:24122/1‑121 (MQ=255)
                                acacGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGtt                                                                                                                                             <  7:24122/121‑1 (MQ=255)
                                 cacGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGt                                                                                                                 >  5:521/1‑148 (MQ=255)
                                   cgcgGTGTCCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGaa                                                                                                              <  1:83472/149‑1 (MQ=255)
                                   cgcgGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGaa                                                                                                              <  7:393296/149‑1 (MQ=255)
                                                gCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACctgctg                                                                                                 <  2:216746/149‑1 (MQ=255)
                                                 cccATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCtgctgc                                                                                                <  5:76030/149‑1 (MQ=255)
                                                   cATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGa                                                                                              >  8:75042/1‑149 (MQ=255)
                                                   cATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGa                                                                                              <  1:279319/149‑1 (MQ=255)
                                                    aTCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCCGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGa                                                                                              >  2:287601/1‑148 (MQ=255)
                                                     tCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGAt                                                                                             >  5:367109/1‑148 (MQ=255)
                                                      cAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATa                                                                                            >  4:118657/1‑148 (MQ=255)
                                                        ggCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAg                                                                                         <  7:337418/149‑1 (MQ=255)
                                                         gCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGCGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                                        >  6:246810/1‑149 (MQ=255)
                                                         gCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                                        <  2:225075/149‑1 (MQ=255)
                                                         gCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAgg                                                                                        <  6:367109/149‑1 (MQ=255)
                                                           ggAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGc                                                                                       >  7:147233/1‑148 (MQ=255)
                                                                gAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACgg                                                                                 <  3:98532/149‑1 (MQ=255)
                                                                   tCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGtt                                                                              >  4:34120/1‑149 (MQ=255)
                                                                     aTTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTc                                                                             <  2:393924/148‑1 (MQ=255)
                                                                     aTTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCg                                                                            <  6:176680/149‑1 (MQ=255)
                                                                         ttGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCAc                                                                         <  3:201193/148‑1 (MQ=255)
                                                                           gTCTTTCGCACGACGAACAAATTCCGTAATGTGTTTTACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGTTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCAccc                                                                       <  1:250771/148‑1 (MQ=255)
                                                                              tttCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCg                                                                      >  6:383054/1‑146 (MQ=255)
                                                                              tttCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCg                                                                      >  5:126062/1‑146 (MQ=255)
                                                                               ttCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTc                                                                                                                         >  6:29222/1‑94 (MQ=255)
                                                                               ttCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTc                                                                                                                         <  5:29222/94‑1 (MQ=255)
                                                                                  gCACGACGAACATATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  <  6:107991/146‑1 (MQ=255)
                                                                                  gCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  >  5:107991/1‑146 (MQ=255)
                                                                                  gCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGc                                                                >  6:155205/1‑148 (MQ=255)
                                                                                    acgacgAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAg                                                             <  6:126062/149‑1 (MQ=255)
                                                                                        cgaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCgg                                                          >  5:34848/1‑148 (MQ=255)
                                                                                         gaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAAt                                                                                                         <  5:312310/100‑1 (MQ=255)
                                                                                         gaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAAt                                                                                                         >  6:312310/1‑100 (MQ=255)
                                                                                         gaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTa                                                        >  7:103205/1‑149 (MQ=255)
                                                                                         gaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTa                                                                                                                                            <  4:278800/65‑1 (MQ=255)
                                                                                         gaACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTa                                                                                                                                            >  3:278800/1‑65 (MQ=255)
                                                                                                           gtTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGt                                                         >  1:310553/1‑130 (MQ=255)
                                                                                                           gtTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGt                                                         <  2:310553/130‑1 (MQ=255)
                                                                                                            tttAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGTGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAgg                                     >  2:34484/1‑149 (MQ=255)
                                                                                                            tttAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAgg                                     >  2:35894/1‑149 (MQ=255)
                                                                                                            tttAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAgg                                     >  4:295989/1‑149 (MQ=255)
                                                                                                               aaCGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGt                                  >  8:142147/1‑149 (MQ=255)
                                                                                                               aaCGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGt                                  >  4:146536/1‑149 (MQ=255)
                                                                                                                aCGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTg                                               >  6:318566/1‑135 (MQ=255)
                                                                                                                aCGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTg                                               <  5:318566/135‑1 (MQ=255)
                                                                                                                  ggAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCt                               >  3:296179/1‑149 (MQ=255)
                                                                                                                  ggAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCt                               <  6:396098/149‑1 (MQ=255)
                                                                                                                   gAGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTg                              >  3:151895/1‑149 (MQ=255)
                                                                                                                    aGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGt                             >  8:250976/1‑149 (MQ=255)
                                                                                                                    aGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  <  1:239621/112‑1 (MQ=255)
                                                                                                                    aGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  >  2:239621/1‑112 (MQ=255)
                                                                                                                    aGCTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGt                             <  4:296179/149‑1 (MQ=255)
                                                                                                                      cTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  >  4:301391/1‑110 (MQ=255)
                                                                                                                      cTGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAaga                                                                  <  3:301391/110‑1 (MQ=255)
                                                                                                                       tGATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACATGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGGTCTGTTCa                          >  5:69411/1‑149 (MQ=255)
                                                                                                                        gATTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCAt                         >  3:276959/1‑149 (MQ=255)
                                                                                                                         aTTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATg                        >  1:239174/1‑149 (MQ=255)
                                                                                                                         aTTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATg                        <  1:287601/149‑1 (MQ=255)
                                                                                                                         aTTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCa                          <  1:35894/147‑1 (MQ=255)
                                                                                                                         aTTTCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATg                        >  6:312802/1‑149 (MQ=255)
                                                                                                                         aTTTCCTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGATGCGATACAGGCAAACGGGTTCGCACACGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATg                        >  5:203837/1‑149 (MQ=255)
                                                                                                                          tttCTTCCAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATg                        <  8:147233/148‑1 (MQ=255)
                                                                                                                                ccAGCATTTTCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCa                    <  3:118657/146‑1 (MQ=255)
                                                                                                                                 cAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGt                >  5:279149/1‑149 (MQ=255)
                                                                                                                                        ttCAGCGCCGATTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGa          <  5:312802/148‑1 (MQ=255)
                                                                                                                                           aGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCCGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCa       <  5:383054/148‑1 (MQ=255)
                                                                                                                                             cgccgaTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGaa    >  6:26296/1‑149 (MQ=255)
                                                                                                                                              gccgcTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGaa    >  3:315722/1‑148 (MQ=255)
                                                                                                                                               ccgcTTCGTTATCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATa  <  6:521/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
CATTACGGTGGCGCGCGGGTCGTAATTTTTGTACACGCGGTGACCGAAGCCCATCAGGCGGAAAGAATCATTTTTGTCTTTCGCACGACGAACAAATTCCGGAATGTGTTTAACGGAGCTGATTTCTTCCAGCATTTTCAGCGCCGCTTCGTTAGCACCGCCGTGCGCAGGTCCCCACAGTGAAGCAATACCTGCTGCGATACAGGCAAACGGGTTCGCACCCGAAGAGCCAGCGGTACGCACGGTGGAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATA  >  NC_000913/753512‑753803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑