New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4219847112 (1.210)4 (0.050) 4/254 7.7 3.8% coding (1252/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
?NC_000913 = 4219854 99 (1.170)coding (1259/1737 nt) aceK isocitrate dehydrogenase kinase/phosphatase
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below/above cutoff threshold.

CGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219690‑4219847
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  <  NC_000913/4219854‑4219707
                                                                                                                                                                                                                                                                                                                  
CGTGGGCAGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATA                                                                                                                                                               <  2:183372/149‑1
  TGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATT                                                                                                                                                             <  5:196995/149‑1
  TGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATT                                                                                                                                                             <  3:168496/149‑1
    GGCCGACTGGCGGACCCCCAGGAGTTTGAAAACTTTGAGCTCGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAATCAGCGGAAAAAATCACCGATCTCGTCGAACAACTTGTGATTCGCCATCTTTATATTGA                                                                                                                                                           <  2:229196/149‑1
      CCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGC                                                                                                                                                         >  1:369191/1‑149
      CCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGC                                                                                                                                                         <  3:87881/149‑1
       CGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCG                                                                                                                                                        >  5:139568/1‑149
        GAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGG                                                                                                                                                       >  5:59573/1‑149
        GAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGG                                                                                                                                                       >  3:251709/1‑149
                          AGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAAT                                                                                                                                     <  3:363834/149‑1
                                            TGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCA                                                                                                                    >  1:4222/1‑148
                                              GAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAA                                                                                                                 <  2:4222/149‑1
                                                                     ATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCACCATCTTTATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATT                                                                                          <  6:183778/149‑1
                                                                                                                                                  ATATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGGGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAA             <  3:133640/149‑1
                                                                                                                                                   TATTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAA             <  5:295883/148‑1
                                                                                                                                                     TTGAGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTC          >  2:213940/1‑149
                                                                                                                                                        AGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTATTTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCT        <  7:347211/148‑1
                                                                                                                                                        AGCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTG       >  4:289626/1‑149
                                                                                                                                                         GCGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGG      <  5:88092/149‑1
                                                                                                                                                          CGGCACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGG     >  6:159748/1‑149
                                                                                                                                                             CACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  >  4:57106/1‑149
                                                                                                                                                                                                                                                                                                                  
CGTGGGCCGAATGGCGGACACCCAGGAGTTTGAAAACTTTGTGCTGGAGAAGCGGCATATTTCCCCGGCATTAATGGAATTACTGCTTCAGGAAGCAGCGGAAAAAATCACCGATCTCGGCGAACAAATTGTGATTCGCCATCTTTATATTGAGCGGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/4219690‑4219847
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑atattgagcggcACCATCCGCCGCTCAATATAAAGATGGCGAATCACAATTTGTTCGCCGAGATCGGTGATTTTTTCCGCTGCTTCCTGAAGCAGTAATTCCATTAATGCCGGGGAAATATGCCGCTTCTCCAGCACAAAGTTTTCAAACTCCTGGGTGT  <  NC_000913/4219854‑4219707

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.