Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,976,8700GA29.7% 190.0 / 99.2 129G198D (GGT→GAT) wzxEO‑antigen translocase
Reads supporting (aligned to +/- strand):  ref base G (46/44);  new base A (22/16);  total (68/61)
Fisher's exact test for biased strand distribution p-value = 5.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.31e-01
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 135 total aligned reads displayed.

CGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATA  >  NC_000913/3976723‑3977016
                                                                                                                                                   |                                                                                                                                                  
cGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACgtggt                                                                                                                                                   <  1:417281/149‑1 (MQ=255)
       gCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATc                                                                                                                                             <  3:145053/148‑1 (MQ=255)
        cTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATccc                                                                                                                                           >  6:161476/1‑149 (MQ=255)
        cTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATccc                                                                                                                                           >  6:342320/1‑149 (MQ=255)
        cTGATTGTCGGCAGCTTGATAGGCGTTCTCGCGTACAACGTCAGTTACCGTATGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGAATCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGAGGTGACATccc                                                                                                                                           >  7:130121/1‑149 (MQ=255)
           aTTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGtt                                                                                                                                        <  3:384041/149‑1 (MQ=255)
                  gCAGCTTGATTGGCGTTCTCGCGTACTACGTGAGTTACCGTTTGGGCGGTTATGTAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTAt                                                                                                                                 <  4:296486/149‑1 (MQ=255)
                  gCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACgtggtg                                                                                                                                                  >  1:74254/1‑132 (MQ=255)
                  gCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTAt                                                                                                                                 >  8:271529/1‑149 (MQ=255)
                  gCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTAt                                                                                                                                 >  2:196156/1‑149 (MQ=255)
                   cAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATc                                                                                                                                >  1:144101/1‑149 (MQ=255)
                   cAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATc                                                                                                                                <  3:34526/149‑1 (MQ=255)
                    aGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCt                                                                                                                               >  1:236317/1‑149 (MQ=255)
                      cTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTg                                                                                                                              >  2:348124/1‑148 (MQ=255)
                      cTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTg                                                                                                                              >  3:354574/1‑148 (MQ=255)
                         gATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGaaa                                                                                                                           <  4:43530/148‑1 (MQ=255)
                            tGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAAcc                                                                                                                         >  3:122260/1‑147 (MQ=255)
                                 ttCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTgg                                                                                                                  <  1:24941/149‑1 (MQ=255)
                                   ctcGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTggg                                                                                                                 <  1:111090/148‑1 (MQ=255)
                                    tcGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTaa                                                                                                                                      >  5:12241/1‑126 (MQ=255)
                                    tcGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTaa                                                                                                                                      <  6:12241/126‑1 (MQ=255)
                                      gcgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATaa                                                                                                             >  7:26942/1‑149 (MQ=255)
                                       cgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAAc                                                                                                            >  8:133910/1‑149 (MQ=255)
                                       cgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGCAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAg                                                                                                                                     >  4:64415/1‑124 (MQ=255)
                                       cgTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGCAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAg                                                                                                                                     <  3:64415/124‑1 (MQ=255)
                                           ctacGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTc                                                                                                        <  1:348124/149‑1 (MQ=255)
                                            tacGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCt                                                                                                       >  2:222174/1‑149 (MQ=255)
                                             acGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACg                                                                                                           >  1:176522/1‑144 (MQ=255)
                                             acGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTg                                                                                                      >  3:95888/1‑149 (MQ=255)
                                             acGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGGCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACg                                                                                                           <  2:176522/144‑1 (MQ=255)
                                              cGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCt                                                                                                       <  2:236317/147‑1 (MQ=255)
                                                tCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggca                                                                                                   <  7:271529/149‑1 (MQ=255)
                                                   gTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATccc                                                                                                                                           >  8:124377/1‑106 (MQ=255)
                                                   gTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATccc                                                                                                                                           <  7:124377/106‑1 (MQ=255)
                                                    ttACCTTTTGGGCGGTTATGAAGGGGCGTTGATGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcaggg                                                                                                >  3:200553/1‑148 (MQ=255)
                                                    ttACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcaggg                                                                                                >  1:291867/1‑148 (MQ=255)
                                                      aCCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCAAGATGGGTTAACTGGCTggcagggaag                                                                                             >  5:33732/1‑149 (MQ=255)
                                                       ccGTTTGGGCGGTTATGACTGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGt                                                                                            <  2:270597/149‑1 (MQ=255)
                                                         gTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATGACGGTCTggcaggg                                                                                                >  3:221707/1‑143 (MQ=255)
                                                         gTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATGACGGTCTggcaggg                                                                                                <  4:221707/143‑1 (MQ=255)
                                                          tttGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGtt                                                                                           >  6:31540/1‑147 (MQ=255)
                                                          tttGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcagggcag                                                                                             <  1:374658/145‑1 (MQ=255)
                                                          tttGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGa                                                                                         <  4:354574/149‑1 (MQ=255)
                                                                     aTGAATTGGCGTTGCTGGGTGTGGCGCTGATTCCCGAGCTGGTGGTAATTGCTGCCGTCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTAc                                                                               <  4:200553/148‑1 (MQ=255)
                                                                     aTGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTAc                                                                               <  5:342320/148‑1 (MQ=255)
                                                                      tGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACg                                                                              <  5:31540/148‑1 (MQ=255)
                                                                        aaGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCt                                                                            >  4:68404/1‑148 (MQ=255)
                                                                           gggCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATg                                                                        <  4:122260/149‑1 (MQ=255)
                                                                                ttGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGtt                                                                   >  3:24280/1‑149 (MQ=255)
                                                                                   cTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGAt                                                                <  5:161476/149‑1 (MQ=255)
                                                                                    tGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCGGGGATAACGGTCTGGCCGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGAt                                                                >  6:103493/1‑148 (MQ=255)
                                                                                      ggTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGa                                                                                         <  6:31798/121‑1 (MQ=255)
                                                                                        tCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGt                                                           <  1:196156/149‑1 (MQ=255)
                                                                                        tCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGt                                                           >  1:41375/1‑149 (MQ=255)
                                                                                          tGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCg                                                         >  7:8248/1‑149 (MQ=255)
                                                                                            gcgcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCGCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGt                                                       <  8:185170/149‑1 (MQ=255)
                                                                                            gcgcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTc                                                          <  4:340718/146‑1 (MQ=255)
                                                                                            gcgcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGGCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGt                                                       <  1:333921/149‑1 (MQ=255)
                                                                                              gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATc                                                                                                                                             <  2:80531/61‑1 (MQ=255)
                                                                                              gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATc                                                                                                                                             >  1:80531/1‑61 (MQ=255)
                                                                                              gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTAc                                                                               <  6:34867/123‑1 (MQ=255)
                                                                                              gcTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTAc                                                                               >  5:34867/1‑123 (MQ=255)
                                                                                                 gATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCt                                                  >  2:398660/1‑149 (MQ=255)
                                                                                                    tCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGc                                               >  7:310793/1‑149 (MQ=255)
                                                                                                     cccGCGCTGGTGGTAATTCCTGCCGCCTTCATGGTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGGCTGGCAGGGCAGTTGAGCAATTTTACGCTCATGGCGTTGATTTCGTCGGTGAGCTTGc                                               <  8:130121/148‑1 (MQ=255)
                                                                                                     cccGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGGCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGc                                               <  5:103493/148‑1 (MQ=255)
                                                                                                        gcgcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGa                                                                                         <  8:156572/103‑1 (MQ=255)
                                                                                                        gcgcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGa                                                                                         >  7:156572/1‑103 (MQ=255)
                                                                                                          gcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGc                                                                      <  8:303299/120‑1 (MQ=255)
                                                                                                          gcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGc                                                                      >  7:303299/1‑120 (MQ=255)
                                                                                                              gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCtt                                      >  8:70372/1‑148 (MQ=255)
                                                                                                              gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTa                                     >  7:70715/1‑149 (MQ=255)
                                                                                                                ggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcagg                                                                                                 >  4:318007/1‑87 (MQ=255)
                                                                                                                ggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTggcagg                                                                                                 <  3:318007/87‑1 (MQ=255)
                                                                                                                ggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAca                                   >  3:214229/1‑149 (MQ=255)
                                                                                                                 gtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcat                                  >  2:217924/1‑149 (MQ=255)
                                                                                                                   aaTTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatca                                >  1:298916/1‑149 (MQ=255)
                                                                                                                    aTTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatcat                               >  6:137500/1‑149 (MQ=255)
                                                                                                                    aTTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatcat                               <  1:217924/149‑1 (MQ=255)
                                                                                                                     ttCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatg                              <  2:41375/149‑1 (MQ=255)
                                                                                                                     ttCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTAcatcat                               >  6:165333/1‑148 (MQ=255)
                                                                                                                     ttCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatg                              <  3:68404/149‑1 (MQ=255)
                                                                                                                     ttCCTGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatg                              <  2:144101/149‑1 (MQ=255)
                                                                                                                        cttccgccATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCATATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatgat                            <  7:20797/145‑1 (MQ=255)
                                                                                                                        cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCatgatg                           >  2:64930/1‑149 (MQ=255)
                                                                                                                         tGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGc                          >  1:179225/1‑149 (MQ=255)
                                                                                                                         tGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGc                          >  5:270101/1‑149 (MQ=255)
                                                                                                                         tGCCGCCATCATGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGc                          >  2:259989/1‑149 (MQ=255)
                                                                                                                           ccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGc                          <  4:24280/147‑1 (MQ=255)
                                                                                                                             gccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGGGACCTTGCCTGGTGCTTACATCATGATGCGTa                       >  2:12910/1‑148 (MQ=255)
                                                                                                                               catcatGTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAAc                    <  6:367788/149‑1 (MQ=255)
                                                                                                                                   atGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgct                <  5:404966/149‑1 (MQ=255)
                                                                                                                                   atGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgc                 <  2:291867/148‑1 (MQ=255)
                                                                                                                                    tGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgctg               >  3:197449/1‑149 (MQ=255)
                                                                                                                                     gTTGATCAAACGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg              >  6:318423/1‑149 (MQ=255)
                                                                                                                                            aaaCGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGgcgc        <  8:70715/148‑1 (MQ=255)
                                                                                                                                             aaCGTGATGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACg                                                                              <  8:304498/77‑1 (MQ=255)
                                                                                                                                                gtgatgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAg      <  7:177282/146‑1 (MQ=255)
                                                                                                                                                gtgatgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAg      >  8:177282/1‑146 (MQ=255)
                                                                                                                                                 tggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGtata  <  1:222174/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CGTTATCGCTGATTGTCGGCAGCTTGATTGGCGTTCTCGCGTACTACGTCAGTTACCGTTTGGGCGGTTATGAAGGGGCGTTGCTGGGTCTGGCGCTGATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATA  >  NC_000913/3976723‑3977016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑