Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,683,472 G→A intergenic (‑25/+158) dctA ← / ← yhjK C4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,683,4720GA99.4% 566.6 / ‑6.0 160intergenic (‑25/+158)dctA/yhjKC4‑dicarboxylic acid, orotate and citrate transporter/cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (85/74);  total (86/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 160 total aligned reads displayed.

CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAAT  >  NC_000913/3683329‑3683616
                                                                                                                                               |                                                                                                                                                
ccGTCTCCAAGCGGTTTCATTTCCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCGTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCtt                                                                                                                                             <  2:298955/149‑1 (MQ=255)
ccGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCtt                                                                                                                                             <  7:176590/149‑1 (MQ=255)
  gTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAACCCACAAGTCTTg                                                                                                                                            >  2:66203/1‑148 (MQ=255)
      ccAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCATGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAgg                                                                                                                                       <  1:184288/149‑1 (MQ=255)
           cGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATg                                                                                                                                  >  4:64876/1‑149 (MQ=255)
            ggTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATgg                                                                                                                                 <  1:77866/149‑1 (MQ=255)
              tttCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATgggg                                                                                                                               >  4:330059/1‑149 (MQ=255)
              tttCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATgggg                                                                                                                               >  2:190906/1‑149 (MQ=255)
              tttCATTTACTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATgggg                                                                                                                               <  6:105740/149‑1 (MQ=255)
               ttCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATgggg                                                                                                                               >  8:82221/1‑148 (MQ=255)
                  aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATg                                                                                                                                  <  6:240330/142‑1 (MQ=255)
                  aTTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTCTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATg                                                                                                                                  >  1:220475/1‑142 (MQ=255)
                       cTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCgg                                                                                                                      >  7:38243/1‑149 (MQ=255)
                            cTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTa                                                                                                                 <  2:134963/149‑1 (MQ=255)
                              aTTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaa                                                                                                                >  6:124402/1‑148 (MQ=255)
                                 tCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAt                                                                                                             <  5:345340/148‑1 (MQ=255)
                                 tCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAAt                                                                                                             <  3:365570/148‑1 (MQ=255)
                                   aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATaa                                                                                                           <  7:82221/148‑1 (MQ=255)
                                   aGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATaa                                                                                                           <  2:176102/148‑1 (MQ=255)
                                     gATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTATTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAacac                                                                                                        >  8:357002/1‑149 (MQ=255)
                                           aaTGGACAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGac                                                                                                  <  8:301061/149‑1 (MQ=255)
                                                       gaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGa                                                                                       >  8:207463/1‑148 (MQ=255)
                                                       gaATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGa                                                                                       >  4:259597/1‑148 (MQ=255)
                                                         aTACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAt                                                                                    <  7:303662/149‑1 (MQ=255)
                                                          tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                   >  3:55582/1‑149 (MQ=255)
                                                          tACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATg                                                                                   >  7:95961/1‑149 (MQ=255)
                                                             cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTaaaa                                                                                                              >  2:173844/1‑119 (MQ=255)
                                                             cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                                >  6:70671/1‑149 (MQ=255)
                                                             cAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAc                                                                                >  8:283621/1‑149 (MQ=255)
                                                               aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCt                                                                              >  6:5221/1‑149 (MQ=255)
                                                               aTGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCt                                                                              <  2:25784/149‑1 (MQ=255)
                                                                      tCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGGGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCa                                                                        <  6:231775/148‑1 (MQ=255)
                                                                        gCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGGTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATa                                                                      <  6:368396/148‑1 (MQ=255)
                                                                          tGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGacaac                                                                                               >  5:185889/1‑121 (MQ=255)
                                                                          tGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGacaac                                                                                               <  6:185889/121‑1 (MQ=255)
                                                                            tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                   <  3:330059/147‑1 (MQ=255)
                                                                            tCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                   <  7:320728/147‑1 (MQ=255)
                                                                              aGGACCTGAAAGTAAATGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGaa                                                                <  3:118532/148‑1 (MQ=255)
                                                                              aGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGaa                                                                <  2:392327/148‑1 (MQ=255)
                                                                                gACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAAc                                                              >  1:233510/1‑148 (MQ=255)
                                                                                gACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGCTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAAc                                                              >  7:148372/1‑148 (MQ=255)
                                                                                   cTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                           >  7:105484/1‑148 (MQ=255)
                                                                                    tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                           >  5:253395/1‑147 (MQ=255)
                                                                                    tGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACaaa                                                           >  7:58017/1‑147 (MQ=255)
                                                                                      aaaGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                        <  4:140405/148‑1 (MQ=255)
                                                                                      aaaGTAAAGGATTTTTAACAGAGAGGTTTTCATAGTGTGTCCTTTAGTAAAACCACAAGTCTTGTAATGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACATCATCACAGAAATTTACCTGGATCATAATTGAAACAAAAAg                                                        <  5:391861/148‑1 (MQ=255)
                                                                                            aaGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaa                                                  >  3:78989/1‑148 (MQ=255)
                                                                                             aGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaaaa                                                <  7:357002/149‑1 (MQ=255)
                                                                                             aGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaaaa                                                <  5:124402/149‑1 (MQ=255)
                                                                                             aGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaa                                                  >  6:122245/1‑147 (MQ=255)
                                                                                             aGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAGCATAATTGAAACAAAAAGGTTaaaaa                                                <  4:211525/149‑1 (MQ=255)
                                                                                               gCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGtt                                                     <  1:313437/142‑1 (MQ=255)
                                                                                               gCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGtt                                                     >  2:313437/1‑142 (MQ=255)
                                                                                                  tttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAAt                                                                                    >  8:164297/1‑108 (MQ=255)
                                                                                                  tttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                            >  4:272471/1‑148 (MQ=255)
                                                                                                   ttAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGttt                                            <  8:81883/147‑1 (MQ=255)
                                                                                                    tAATCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                          <  5:239505/148‑1 (MQ=255)
                                                                                                     aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGc                                        >  5:73894/1‑149 (MQ=255)
                                                                                                     aaaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGGAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTATGAGc                                        >  6:268408/1‑149 (MQ=255)
                                                                                                      aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAt                                                                          <  5:247448/114‑1 (MQ=255)
                                                                                                      aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGAt                                                                          >  6:247448/1‑114 (MQ=255)
                                                                                                      aaCAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCt                                       <  5:70671/149‑1 (MQ=255)
                                                                                                        cAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTgg                                     <  8:95961/149‑1 (MQ=255)
                                                                                                        cAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTgg                                     <  4:114503/149‑1 (MQ=255)
                                                                                                         agagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAg                                                        <  5:215407/129‑1 (MQ=255)
                                                                                                         agagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc                                    >  5:233194/1‑149 (MQ=255)
                                                                                                         agagagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGc                                    >  4:186560/1‑149 (MQ=255)
                                                                                                           agagGTGTTCATCGGGTGTCCTTTAGTAACACCACAAGTCTTGTAAGGTTATGTGGTACCTGCGGCCTTAAAATACCACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTc                                  <  4:197647/149‑1 (MQ=255)
                                                                                                            gagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                   <  5:38417/115‑1 (MQ=255)
                                                                                                            gagGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAAtt                                                                   >  6:38417/1‑115 (MQ=255)
                                                                                                             agGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                          >  1:318376/1‑139 (MQ=255)
                                                                                                             agGTTTTCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGa                                          <  2:318376/139‑1 (MQ=255)
                                                                                                                ttttCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGcaca                             <  5:122245/149‑1 (MQ=255)
                                                                                                                   tCATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                          >  8:39910/1‑149 (MQ=255)
                                                                                                                    cATAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTaaaaa                                                >  3:248609/1‑126 (MQ=255)
                                                                                                                     aTAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAcc                         <  6:316645/148‑1 (MQ=255)
                                                                                                                     aTAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAAc                          >  8:405637/1‑147 (MQ=255)
                                                                                                                      tAGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAg                       >  4:380224/1‑149 (MQ=255)
                                                                                                                       aGGGTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCCGACACCATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGc                      >  6:269940/1‑149 (MQ=255)
                                                                                                                        gggTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTAcc                                                                               >  1:29542/1‑91 (MQ=255)
                                                                                                                         ggTGTCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCAc                    >  3:359808/1‑149 (MQ=255)
                                                                                                                            gtCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt                 >  2:56954/1‑149 (MQ=255)
                                                                                                                             tCCTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttt                 <  3:272471/148‑1 (MQ=255)
                                                                                                                               cTTTAGTAACACCACAAGACATGTACCGTTATGGGGTACCTGCGGCTTTAAAATAACACCCAGACAACATCACAGAAATGTACGTGGATCATAATTGAAACAAAATGGGTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACttttt               <  2:145885/148‑1 (MQ=255)
                                                                                                                               cTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa              <  7:207463/149‑1 (MQ=255)
                                                                                                                               cTTTAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCCGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGATACAAAAAGGTTTAAAAGATTGACCTGGATAGCACAAACCAGCaatataa               >  1:119286/1‑142 (MQ=255)
                                                                                                                                tttAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa              >  7:399025/1‑148 (MQ=255)
                                                                                                                                tttAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa              >  3:287482/1‑148 (MQ=255)
                                                                                                                                tttAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCGGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa              >  2:383271/1‑148 (MQ=255)
                                                                                                                                 ttAGTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTa              >  6:190939/1‑147 (MQ=255)
                                                                                                                                    gTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt         >  8:21309/1‑149 (MQ=255)
                                                                                                                                    gTAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtt         <  5:190939/149‑1 (MQ=255)
                                                                                                                                     tAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt        <  3:359473/149‑1 (MQ=255)
                                                                                                                                     tAAAACCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGttt        >  6:55748/1‑149 (MQ=255)
                                                                                                                                       aaaCCACAAGTCTTGTAAGGTTATGGGGTACCTGTGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt       <  3:210835/148‑1 (MQ=255)
                                                                                                                                       aaaCCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt       <  7:43832/148‑1 (MQ=255)
                                                                                                                                       aaaCCACAAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt       <  2:233510/148‑1 (MQ=255)
                                                                                                                                           cacaAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAAt  >  1:366588/1‑149 (MQ=255)
                                                                                                                                            acaAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGtttt       >  7:176939/1‑143 (MQ=255)
                                                                                                                                            acaAGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAAt  >  1:273612/1‑148 (MQ=255)
                                                                                                                                               |                                                                                                                                                
CCGTCGCCAAGCGGTTTCATTTGCTCGCCTATTTCAGGATAGAAATGGCCAAGGAGAATACCAATGGCTATCGCTGTCAGGACCTGAAAGTAAAGGCTTTTAAACAGAGAGGTTTTCATAGGGTGTCCTTTAGTAAAACCACAGGTCTTGTAAGGTTATGGGGTACCTGCGGCCTTAAAATAACACCCAGACAACATCACAGAAATGTACCTGGATCATAATTGAAACAAAAAGGTTAAAAAGTTTGAGCTGGCTCGCACAAACCAGCACTTTTTAAAGTTTTGTAAT  >  NC_000913/3683329‑3683616

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: