Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,862,760 C→T R142R (CGC→CGT ygbK → FliA‑regulated DUF1537 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,862,7600CT96.8% 288.3 / ‑6.6 94R142R (CGC→CGTygbKFliA‑regulated DUF1537 family protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (45/46);  minor base G (0/3);  total (45/49)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGC  >  NC_000913/2862622‑2862902
                                                                                                                                          |                                                                                                                                              
cccGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCgg                                                                                                                                      >  7:65666/1‑149 (MQ=255)
 ccGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCgg                                                                                                                                      >  3:177129/1‑148 (MQ=255)
 ccGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCgg                                                                                                                                      >  8:319521/1‑148 (MQ=255)
    gTTACCGATGCCTTAATGGATGCTCTCGCCACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCCACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTaaa                                                                                                                                  >  6:278065/1‑149 (MQ=255)
    gTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTaaa                                                                                                                                  >  8:88656/1‑149 (MQ=255)
     ttACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCAccc                                                                                                                                        >  6:140925/1‑142 (MQ=255)
     ttACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCAccc                                                                                                                                        <  5:140925/142‑1 (MQ=255)
         cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                             <  7:319521/149‑1 (MQ=255)
         cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                             <  2:329306/149‑1 (MQ=255)
         cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCa                                                                                                                             <  2:248126/149‑1 (MQ=255)
         cGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCATGCCGGTCAACGGACGAACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATccc                                                                                                                              >  6:308968/1‑148 (MQ=255)
           aTGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCAt                                                                                                                            >  4:204232/1‑148 (MQ=255)
              ccTTAATGGATGCGCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGGCATCACCCGGTAAATCCCATGAcc                                                                                                                        <  6:225912/149‑1 (MQ=255)
               cTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGAcc                                                                                                                        >  3:90832/1‑148 (MQ=255)
                ttAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGa                                                                                                                      >  1:106922/1‑149 (MQ=255)
                  aaTGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGa                                                                                                                      >  6:168638/1‑147 (MQ=255)
                   aTGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAg                                                                                                                   >  3:316556/1‑149 (MQ=255)
                        tGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATc                                                                                                              <  5:249213/149‑1 (MQ=255)
                           tctcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTc                                                                                                           >  4:54497/1‑149 (MQ=255)
                           tctcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTc                                                                                                           >  1:268863/1‑149 (MQ=255)
                            ctcGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTcc                                                                                                          >  8:290435/1‑149 (MQ=255)
                                  acGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCt                                                                                                    <  4:331632/149‑1 (MQ=255)
                                  acGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCt                                                                                                    >  2:344660/1‑149 (MQ=255)
                                   cGCCGTTTACGTTCTTCTCTCCGGCCATGCCGGTCAAAGGACGTACGGTTTATCAGGTGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGTATGCGTCATCACACGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTg                                                                                                   <  5:64166/149‑1 (MQ=255)
                                    gCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGCCGGCTATCTTCCCCGTCTgg                                                                                                  >  3:157069/1‑149 (MQ=255)
                                     ccGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGATTCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGt                                                                                                 <  4:316556/149‑1 (MQ=255)
                                     ccGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGt                                                                                                 <  2:106922/149‑1 (MQ=255)
                                     ccGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGt                                                                                                 <  2:268863/149‑1 (MQ=255)
                                          tACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGTGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGaa                                                                                             <  3:53009/148‑1 (MQ=255)
                                          tACGGTCTTCTCTCCGGCCCTACCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGaa                                                                                             <  3:111183/148‑1 (MQ=255)
                                            cGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAgc                                                                                           <  3:204232/148‑1 (MQ=255)
                                            cGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAgc                                                                                           <  8:65666/148‑1 (MQ=255)
                                             ggTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAgcg                                                                                          >  8:43125/1‑148 (MQ=255)
                                             ggTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAgcg                                                                                          >  4:29129/1‑148 (MQ=255)
                                               tcttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCAGGCCGAATCCGGGATGCGTCATCCCCCGGTAAATCCCATGACCGCCAGCTAGCTTCCCCGGCTGGGTGCAGCGCaa                                                                                       >  4:301273/1‑149 (MQ=255)
                                                 ttctCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATc                                                                                     >  2:133493/1‑149 (MQ=255)
                                                  tctctcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCTGTCTGGTTGAAGCGCAATc                                                                                     >  5:149551/1‑148 (MQ=255)
                                                   ctctcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATcc                                                                                    <  6:309346/148‑1 (MQ=255)
                                                   ctctcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCca                                                                                   >  7:93530/1‑149 (MQ=255)
                                                      tcCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAg                                                                                >  1:238754/1‑149 (MQ=255)
                                                        cGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCcaca                                                                                 <  8:185647/146‑1 (MQ=255)
                                                        cGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAgg                                                                               <  4:90835/148‑1 (MQ=255)
                                                           cccTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGGTGAAGCGCAATCCACAGGgcgc                                                                           <  5:304929/149‑1 (MQ=255)
                                                              tGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTgc                                                                        >  8:113263/1‑149 (MQ=255)
                                                              tGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTgc                                                                        >  7:171912/1‑149 (MQ=255)
                                                                ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTccc                                                                                                         <  1:152255/114‑1 (MQ=255)
                                                                ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTccc                                                                                                         >  2:152255/1‑114 (MQ=255)
                                                                ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTgcg                                                                       <  8:33076/148‑1 (MQ=255)
                                                                ccGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGAAGCGTCATGACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTgcgg                                                                      >  6:36100/1‑149 (MQ=255)
                                                                         ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTTGTTTc                                                             <  7:113263/149‑1 (MQ=255)
                                                                         ggACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                             <  8:171912/149‑1 (MQ=255)
                                                                          gACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCg                                                            <  4:177129/149‑1 (MQ=255)
                                                                              tACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGcac                                                        <  7:290435/149‑1 (MQ=255)
                                                                               aCGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGcaca                                                       >  2:74994/1‑149 (MQ=255)
                                                                                cGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACAt                                                      <  2:106986/149‑1 (MQ=255)
                                                                                 ggTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                             <  1:336991/141‑1 (MQ=255)
                                                                                 ggTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                             >  2:336991/1‑141 (MQ=255)
                                                                                      aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGc                                                          <  1:278546/139‑1 (MQ=255)
                                                                                      aTCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGc                                                          >  2:278546/1‑139 (MQ=255)
                                                                                       tCATGGGTATTTGTTCGTTATGAATCAAGTGGTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTc                                                <  5:70431/148‑1 (MQ=255)
                                                                                       tCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCTCTGCGGCGTCGTTTCGGCACATGTTTTc                                                <  7:323762/148‑1 (MQ=255)
                                                                                         aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCg                                                            >  5:31935/1‑134 (MQ=255)
                                                                                         aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCg                                                            <  6:31935/134‑1 (MQ=255)
                                                                                         aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGaa                                             <  8:93530/149‑1 (MQ=255)
                                                                                         aGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGaa                                             >  4:41648/1‑149 (MQ=255)
                                                                                           gggTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAc                                            >  6:72422/1‑148 (MQ=255)
                                                                                             gTATTTGTTCGTAAATAAACCACTGCCGGCCGTATCCGGGCTGCCGCATCACCCGGTAAAACCCATGGCCGCCCCCTTTCATCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAg                                         <  7:188145/149‑1 (MQ=255)
                                                                                                tttGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCTGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                       <  6:149551/148‑1 (MQ=255)
                                                                                                tttGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                       <  4:90832/148‑1 (MQ=255)
                                                                                                    ttCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTgg                                   <  1:133493/148‑1 (MQ=255)
                                                                                                    ttCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGa                                  <  3:41648/149‑1 (MQ=255)
                                                                                                     tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGAt                                 >  7:263283/1‑149 (MQ=255)
                                                                                                     tCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTACGAACAAGGTGTGGAt                                 >  2:15402/1‑149 (MQ=255)
                                                                                                      cGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgtgggatcg                                >  5:142742/1‑143 (MQ=255)
                                                                                                      cGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGgt                                       <  6:142742/142‑1 (MQ=255)
                                                                                                       gTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                             <  7:283190/119‑1 (MQ=255)
                                                                                                       gTAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTc                                                             >  8:283190/1‑119 (MQ=255)
                                                                                                        tAATGAATCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGcc                              >  2:352525/1‑149 (MQ=255)
                                                                                                             aaTCAACTGCTGGCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTc                          <  1:74994/148‑1 (MQ=255)
                                                                                                               tCAACTGCTGGCCGAATACGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGATATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCAGTCGCGACCTTACGGCACATGTTTTCGAAGAAGGAGTGGATGCCGTACGt                        >  5:265202/1‑148 (MQ=255)
                                                                                                                 aaCTGCTGGACTAATCCGTGATGCGTCATCACCCTGTAAATCACATGACCGACAGCTATCTTCCCCTTCTTGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCTGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCa                      <  2:357980/148‑1 (MQ=255)
                                                                                                                         gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtttt                                                 <  2:117516/113‑1 (MQ=255)
                                                                                                                         gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGtttt                                                 >  1:117516/1‑113 (MQ=255)
                                                                                                                         gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGt                        >  5:275437/1‑138 (MQ=255)
                                                                                                                         gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGt                        <  6:275437/138‑1 (MQ=255)
                                                                                                                         gCCGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGc             <  5:308968/149‑1 (MQ=255)
                                                                                                                          ccGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAgg                                                                               >  8:100317/1‑82 (MQ=255)
                                                                                                                          ccGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAgg                                                                               <  7:100317/82‑1 (MQ=255)
                                                                                                                          ccGAATCCGGGATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCt            <  4:170292/149‑1 (MQ=255)
                                                                                                                           cgacgccGGGATGCGGCATCACCCGGTAAATCCCAGGACCGACAGCTCTCTTCCCCGTCGGGTTGAAGCGCCAGCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGGGTGGATGCCGTTCGTCAAGAGCTGGCTc           <  3:301273/144‑1 (MQ=255)
                                                                                                                                  gggATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTa      >  8:276203/1‑147 (MQ=255)
                                                                                                                                   ggATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAg   <  3:29129/149‑1 (MQ=255)
                                                                                                                                    gATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGTCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAAAAATGTGTTGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGc  <  2:238754/149‑1 (MQ=255)
                                                                                                                                    gATGCGTCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAg   >  7:243419/1‑148 (MQ=255)
                                                                                                                                       gcgtCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGc  >  3:51449/1‑146 (MQ=255)
                                                                                                                                          |                                                                                                                                              
CCCGGTTACCGATGCCTTAATGGATGCTCTCGACACGCCGTTTACGGTCTTCTCTCCGGCCCTGCCGGTCAACGGACGTACGGTTTATCAGGGGTATTTGTTCGTAATGAATCAACTGCTGGCCGAATCCGGGATGCGCCATCACCCGGTAAATCCCATGACCGACAGCTATCTTCCCCGTCTGGTTGAAGCGCAATCCACAGGGCGCTGCGGCGTCGTTTCGGCACATGTTTTCGAACAAGGTGTGGATGCCGTTCGTCAAGAGCTGGCTCGCTTACAGC  >  NC_000913/2862622‑2862902

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: