breseq version 0.33.1 revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 755,790 | G→A | W77* (TGG→TGA) | sdhD → | succinate dehydrogenase, membrane subunit, binds cytochrome b556 |
JC JC | 1,293,032 | IS1 (–) +8 bp | intergenic (‑110/‑488) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
MC JC | 1,299,499 | Δ1,199 bp | intergenic (+254/‑485) | ychE → / → oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
JC | 4,001,645 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,543 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 761505 | 762752 | 1248 | 7 [5] | [5] 7 | [sucA]–sucB | [sucA],sucB |
* | * | ÷ | NC_000913 | 2066276–2066581 | 2066581 | 1–306 | 6 [5] | [5] 7 | [insH1] | [insH1] |
* | * | ÷ | NC_000913 | 3423762–3424234 | 3424520–3424240 | 7–759 | 6 [3] | [5] 6 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 224374 | NA (NA) | 6 (0.190) | 6/246 | 2.7 | NA | noncoding (604/1542 nt) | rrsH | 16S ribosomal RNA of rrnH operon |
? | NC_000913 | = 224403 | NA (NA) | noncoding (633/1542 nt) | rrsH | 16S ribosomal RNA of rrnH operon | |||||
* | ? | NC_000913 | = 381669 | NA (NA) | 8 (0.250) | 7/246 | 2.5 | NA | noncoding (410/1331 nt) | IS2 | repeat region |
? | NC_000913 | = 381695 | NA (NA) | noncoding (436/1331 nt) | IS2 | repeat region | |||||
* | ? | NC_000913 | = 409055 | NA (NA) | 8 (0.250) | 8/246 | 2.2 | NA | noncoding (4/37 nt) | REP34 (repetitive extragenic palindromic) element; contains 1 REP sequences | REP34 (repetitive extragenic palindromic) element; contains 1 REP sequences |
? | NC_000913 | = 409088 | NA (NA) | noncoding (37/37 nt) | REP34 (repetitive extragenic palindromic) element; contains 1 REP sequences | REP34 (repetitive extragenic palindromic) element; contains 1 REP sequences | |||||
* | ? | NC_000913 | 1207790 = | 23 (0.680) | 8 (0.270) | 8/228 | 2.1 | 29.5% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 18 (0.600) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related |