Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	512,655	0	T	G	23.2%	138.0 / 12.8	69	V27G (GTG→GGG)	ybaT	putative amino acid transporter
Reads supporting (aligned to +/- strand):  ref base T (42/11);  new base G (0/16);  total (42/27)
Fisher's exact test for biased strand distribution p-value = 6.81e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TTCGCGGTCAAAAAATGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAA  >  NC_000913/512509‑512796                                                                                                                                                   |                                                                                                                                              ttCGCGGTCAAAAAATGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAAtggtgg                                                                                                                                             <  2:58844/149‑1 (MQ=255)    gcgGTCAAAAAATGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATACCGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTtgggg                                                                                                                                          >  2:315835/1‑149 (MQ=255)          aaaaaaTGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGa                                                                                                                                    >  4:294441/1‑149 (MQ=255)          aaaaaaTGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGa                                                                                                                                    >  1:251658/1‑149 (MQ=255)            aaaaTGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGT‑GGGGCGGGGATCt                                                                                                                                 >  8:317047/1‑149 (MQ=255)             aaaTGGTGGCATCGGTCGCTAAGCAACTCGGCTATACCGTGTTTAAGGGCTGATCATGATGAACCCGGAAGGTAATAACGGTAACAAGCCTCTCGGTCTATGGAACGTCGTTTCGATCGGCATTGGGGCAATGGTGGGGGCGGGGAGCt                                                                                                                                 >  8:335359/1‑149 (MQ=255)                tggtgcCATCGGTCGATAAGCAACTCGGCTATACCGTGTTTAAGGGCTGATCATGATCAAACGGGATGGTAATAACGGTAACAAACCTGTGGGTCTTTGGAACTTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTcg                                                                                                                              <  5:43662/149‑1 (MQ=255)                  gtggCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTcgcg                                                                                                                            >  2:145837/1‑149 (MQ=255)                     gCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACAAGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCgctg                                                                                                                         >  4:156022/1‑149 (MQ=255)                       aTCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCgctgct                                                                                                                       >  6:30868/1‑149 (MQ=255)                          ggaccatAAGCAACTCGGCTATAACGGGTTTAAGGGGTGACCAGGAGCAACACGGAGGGAAATACGGGTAACACCCGGCTCGGGCATTGCAACGGGGTTTCAATCGGAATGGGGGCAATGGGGGGGGCGGGCATCTTCGCGCTGCTggg                                                                                                                    <  4:225932/143‑1 (MQ=255)                                tAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGc                                                                                                              >  8:244163/1‑149 (MQ=255)                                     aaCTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCAt                                                                                                         >  1:136543/1‑149 (MQ=255)                                        tCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGc                                                                                                      >  3:241764/1‑149 (MQ=255)                                        tCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGc                                                                                                      >  2:144102/1‑149 (MQ=255)                                          ggCTATAACGTGTTTAAGGGCTGATCATGATCACCACGCAAGGTAAAACCGGTAACAAACCTCTCGGTCTATGTACCGGCGTTTCCAGCGGCATTGGGGCAATGGGGGGGGCGGCGATCTTCGCGCTGCTGGGGCAGGCTGCATTGc                                                                                                      <  4:234164/147‑1 (MQ=255)                                             tataACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTgggg                                                                                                                   >  1:44600/1‑131 (MQ=255)                                             tataACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATg                                                                                                 >  1:194375/1‑149 (MQ=255)                                                  cGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTCGGGCCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGc                                                                                            >  1:270309/1‑149 (MQ=255)                                                     gtTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTc                                                                                         >  5:134395/1‑149 (MQ=255)                                                         aaGGGCTGATCTTGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCCCGCTGCTGGGGCAGGCTGCATTGCTAATGCAAGCCTCGAcc                                                                                     >  2:220365/1‑149 (MQ=255)                                                         aaGGGCTGATCATCAGCAACAGGGACGGTAATACGGGTAACATACCTAGGGGTCTATGGACGGCCGTTCCATTGGGCTTTGGGGCAATGGGGGGGGGGGGTAGCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGAc                                                                                      <  8:96724/148‑1 (MQ=255)                                                          aGGGCGTATCATGATCAACACGGAAGGTAATAACGGAAACAAACCTCTCGGGCTATGGAACGGCGTTTCCTTCGGCATTGGGGCAATGGGGGGGGCGGGGAGCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCt                                                                                    <  1:366538/149‑1 (MQ=255)                                                            ggCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGTATTGCTAATGGAAGCCTCGACCTgg                                                                                  >  7:264185/1‑149 (MQ=255)                                                            ggCTGAGCATGATGACCCCGGAAGGTAATAAGGGTAACAAACCTCTCGGTCTTTGGACCGTCGTTGCCATCGGCATTGGGGCAAGGGGGGGGGCGGGGATCTTCGCGCTGCGGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCCgg                                                                                  <  5:30868/149‑1 (MQ=255)                                                                 aTCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGc                                                                             >  2:148743/1‑149 (MQ=255)                                                                     tgatgaACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCttt                                                                         >  1:3788/1‑149 (MQ=255)                                                                         gaACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCtt                                                                     >  7:198045/1‑149 (MQ=255)                                                                           acacGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCtttt                                                                   >  1:169764/1‑149 (MQ=255)                                                                            cacGGAAGGTAATACCGGTAACAACCCGCTCGGGCTATGGAACGGCGTTTCCAGCGGCATTGGGGCAATGGGGGGGGCGGGGAGCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTg                                                                  <  1:145837/149‑1 (MQ=255)                                                                             acGGCAGGTAATACCGGTAACCACCCGGCCGGGATATGCACCGGCGTGGCCATCGCCCTGGGGGCATGGGGGGGGGCGGCGAGCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAAGCGAAGCCTCGACCTGGGGCGCCTTTGCTTTTgg                                                                 <  1:66058/149‑1 (MQ=255)                                                                               ggAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCg                                                               >  2:92372/1‑149 (MQ=255)                                                                               ggAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCg                                                               >  7:98165/1‑149 (MQ=255)                                                                                gAATGTAATAACGGGAACAAACCTGACGGGATATGGAACGGCGTTTCCATCGGCATTGGGGCAATGGGGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCgg                                                              <  3:231572/149‑1 (MQ=255)                                                                                gAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCgg                                                              >  5:376680/1‑149 (MQ=255)                                                                                       ataaCGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATtgtg                                                       >  3:239770/1‑149 (MQ=255)                                                                                            ggTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGAt                                                  <  1:92372/149‑1 (MQ=255)                                                                                                aCAACCCTCTGGGTCTATGGACGGGCGTTTCCATGGGCATTGGGGAATTGGGGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGttt                                              <  6:134395/149‑1 (MQ=255)                                                                                                 cAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGtttt                                             >  5:184246/1‑149 (MQ=255)                                                                                                 cAAACCTCTAGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGAAATGGTGTGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGATAATGGAAGCCTCGACATGGTGAGCCTGTCAGTGTGGCGGTATTGGGGCGATGgttt                                             >  2:329789/1‑149 (MQ=255)                                                                                                  aaaCCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTc                                            >  5:217852/1‑149 (MQ=255)                                                                                                  aaaCCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTc                                            >  7:343767/1‑149 (MQ=255)                                                                                                    aCCTCTCGGTCTAGGGACCGTCGTTGCCATCGGCATTGGGGCAAGGGGGGGGGCGGGGATCTGCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCg                                          <  7:335359/149‑1 (MQ=255)                                                                                                     ccTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGGGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGGCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCgg                                         <  2:136543/149‑1 (MQ=255)                                                                                                      ctctCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGt                                        >  3:189519/1‑149 (MQ=255)                                                                                                       tctcGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGtt                                       >  4:2497/1‑149 (MQ=255)                                                                                                        ctcGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTa                                      >  5:372270/1‑149 (MQ=255)                                                                                                         tcGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCGGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTAt                                     >  3:157596/1‑149 (MQ=255)                                                                                                           gggcTATGGAACGTCGTTTCCATCGGCTTTGGGGAAATGGGGGGGGCGGGGATCTTCGCGCGGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGc                                   <  3:294441/146‑1 (MQ=255)                                                                                                           ggTCTTTGGAACTGCGTTTCCAGGGTCTTTGGGGCAATGGTGGGGGGGGGGATCTTCGCGCGGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTa                                      <  6:376680/146‑1 (MQ=255)                                                                                                               tATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTAt                               >  4:83891/1‑149 (MQ=255)                                                                                                                aTGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTACGGTTATGCCTATg                              >  5:96130/1‑149 (MQ=255)                                                                                                                 tGGAACGTCGTTTCCATCGGCATTGGGGCATTGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATgc                             >  7:305189/1‑149 (MQ=255)                                                                                                                 tGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATgc                             >  3:367851/1‑149 (MQ=255)                                                                                                                  gcaaCGGCTTTTCCATGGGCATGGGGGCAATGGGGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATgc                             <  2:3788/146‑1 (MQ=255)                                                                                                                     aCGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTAt                                     >  2:146095/1‑137 (MQ=255)                                                                                                                     aCGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTAt                                     <  1:146095/137‑1 (MQ=255)                                                                                                                         cgtTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTgg                     <  1:148743/149‑1 (MQ=255)                                                                                                                          gtTTCCATTGGCTTTTGGGCAATGGTGGGGGGGGGGTTCTTCGGGCTGGTTGGGCAGGCTGCATTTCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTggg                    <  1:315835/149‑1 (MQ=255)                                                                                                                           tttCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTgggg                   <  8:98165/149‑1 (MQ=255)                                                                                                                           tttCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTgggg                   <  6:217852/149‑1 (MQ=255)                                                                                                                           tttCCATCGGCATTGGGCCAATGGGGGGGGCGGGGCGCTTCGCGCTGCTGGGGCAGGCTGCCTTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTGTTCCGGTTATGCCTATGCGCGTCTgggg                   <  4:241764/149‑1 (MQ=255)                                                                                                                               ccgcGGCATGGGGGCATGGGGGGGGGCGGGCAGCTTCGCGCTGCTGGGGCAGGCTGTATTGCTAATGGAAGCCTCGACCTGGGGCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGa               <  8:264185/146‑1 (MQ=255)                                                                                                                               cATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGTAATCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGc                 >  1:31961/1‑147 (MQ=255)                                                                                                                               cATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGa               <  1:144102/149‑1 (MQ=255)                                                                                                                                aTCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATtg                                                         <  7:194025/106‑1 (MQ=255)                                                                                                                                aTCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATtg                                                         >  8:194025/1‑106 (MQ=255)                                                                                                                                    gCATTGGGGCAAGGGGGGGGGGGGTGATCGTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTAt          <  4:367851/149‑1 (MQ=255)                                                                                                                                      aTTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGGCTGGGGGCGAGCTATcc        <  6:372270/149‑1 (MQ=255)                                                                                                                                            gCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCaa  >  3:247832/1‑149 (MQ=255)                                                                                                                                                   |                                                                                                                                              TTCGCGGTCAAAAAATGGTGGCATCGGTCGCTAAGCAACTCGGCTATAACGTGTTTAAGGGCTGATCATGATGAACACGGAAGGTAATAACGGTAACAAACCTCTCGGTCTATGGAACGTCGTTTCCATCGGCATTGGGGCAATGGTGGGGGCGGGGATCTTCGCGCTGCTGGGGCAGGCTGCATTGCTAATGGAAGCCTCGACCTGGGTCGCCTTTGCTTTTGGCGGTATTGTGGCGATGTTTTCCGGTTATGCCTATGCGCGTCTGGGGGCGAGCTATCCCAGCAA  >  NC_000913/512509‑512796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑