Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,620,245	C→A	P215T (CCG→ACG)	deoB →	phosphopentomutase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,620,245	0	C	A	100.0%	131.4 / NA	38	P215T (CCG→ACG)	deoB	phosphopentomutase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (19/19);  total (19/19)

TTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGA  >  NC_000913/4620104‑4620387                                                                                                                                              |                                                                                                                                               ttCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTAt                                                                                                                                         >  1:67496/1‑149 (MQ=255)                 aCTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCg                                                                                                                           <  2:67496/147‑1 (MQ=255)                   tCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGc                                                                                                                             >  6:120718/1‑143 (MQ=255)                   tCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGc                                                                                                                             <  5:120718/143‑1 (MQ=255)                     cGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTaa                                                                                                                       <  2:149679/147‑1 (MQ=255)                                   cctgccATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGc                                                                                                               <  3:142157/141‑1 (MQ=255)                                   cctgccATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGc                                                                                                               >  4:142157/1‑141 (MQ=255)                                    ctgccATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                         <  1:8346/146‑1 (MQ=255)                                    ctgccATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCg                                                                                                         >  2:8346/1‑146 (MQ=255)                                       ccATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAAcc                                                                                                   >  3:29032/1‑149 (MQ=255)                                          tGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTc                                                                                                >  7:9759/1‑149 (MQ=255)                                            aagaaACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCa                                                                                               <  5:97167/148‑1 (MQ=255)                                            aagaaACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCAc                                                                                              <  4:29032/149‑1 (MQ=255)                                                aaCTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGAcc                                                                                          >  2:170744/1‑149 (MQ=255)                                                 aCTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCt                                                                                         >  8:49633/1‑149 (MQ=255)                                                        gTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGtt                                                                                  <  3:8565/149‑1 (MQ=255)                                                             gATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAgcc                                                                             >  8:85780/1‑149 (MQ=255)                                                                    tctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGttt                                                                                                                                           <  6:17457/80‑1 (MQ=255)                                                                    tctACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGttt                                                                                                                                           >  5:17457/1‑80 (MQ=255)                                                                        cGAACTGTGCGAACTCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGATCCGCCAGCac                                                                     <  2:51820/146‑1 (MQ=255)                                                                          aaCTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCaccgacc                                                                >  3:125914/1‑149 (MQ=255)                                                                          aaCTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCaccgacc                                                                >  3:123819/1‑149 (MQ=255)                                                                               tgCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGtgct                                                           <  7:49633/149‑1 (MQ=255)                                                                               tgCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGtg                                                             <  7:85780/147‑1 (MQ=255)                                                                                  gAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTCTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTc                                                                                                >  3:52098/1‑109 (MQ=255)                                                                                  gAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTCTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTc                                                                                                <  4:52098/109‑1 (MQ=255)                                                                                             tGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGtt                                              >  1:147953/1‑148 (MQ=255)                                                                                                 gagCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTtgatga                                         <  4:125914/149‑1 (MQ=255)                                                                                                 gagCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTtgatga                                         <  4:123819/149‑1 (MQ=255)                                                                                                         cAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACg                                 >  6:1656/1‑149 (MQ=255)                                                                                                                    aCAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAAcc                                                                                                   >  5:20782/1‑72 (MQ=255)                                                                                                                    aCAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAAcc                                                                                                   <  6:20782/72‑1 (MQ=255)                                                                                                                     cAATATCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGttt                     <  2:147953/149‑1 (MQ=255)                                                                                                                        tatCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTtgat                                           >  2:14509/1‑124 (MQ=255)                                                                                                                        tatCGGTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTtgat                                           <  1:14509/124‑1 (MQ=255)                                                                                                                             gTCGTGTTATCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGCCCGTGCTGCAGAAACCGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCgg              >  8:134164/1‑148 (MQ=255)                                                                                                                                      tCGCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGc     <  1:170744/148‑1 (MQ=255)                                                                                                                                        gCTCGTACGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGa  >  8:82402/1‑149 (MQ=255)                                                                                                                                              |                                                                                                                                               TTCTATACCTCCGCTGACTCCGTGTTCCAGATTGCCTGCCATGAAGAAACTTTCGGTCTGGATAAACTCTACGAACTGTGCGAAATCGCCCGTGAAGAGCTGACCAACGGCGGCTACAATATCGGTCGTGTTATCGCTCGTCCGTTTATCGGCGACAAAGCCGGTAACTTCCAGCGTACCGGTAACCGTCACGACCTGGCTGTTGAGCCGCCAGCACCGACCGTGCTGCAGAAACTGGTTGATGAAAAACACGGCCAGGTGGTTTCTGTCGGTAAAATTGCGGA  >  NC_000913/4620104‑4620387

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑