Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,044,390 (T)6→5 coding (192/1431 nt) yihF → DUF945 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,044,3850T.100.0% 274.2 / NA 61coding (187/1431 nt)yihFDUF945 family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (31/30);  total (31/30)

CCGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATG  >  NC_000913/4044239‑4044516
                                                                                                                                                  |                                                                                                                                   
ccGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTTTTAAGGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCttt                                                                                                                                   >  1:152932/1‑149 (MQ=255)
        tGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTcc                                                                                                                          >  6:91521/1‑149 (MQ=255)
             tggtggCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGttt                                                                                                                     >  2:79830/1‑149 (MQ=255)
                 ggCACATGGTACAGAGGTACGCAAATTCAGCGTGGTGGCGAAAAATTTATTAAAGATTTTAACGAGGCTAAATAGAAAGGTGTACTTGGCGACGATATGACGGTAAGTTATCTATATTGAGACAAGGGCTTTTTATGTCCCGGTTTca                                                                                                                  >  4:141188/1‑148 (MQ=255)
                 ggCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTca                                                                                                                  >  6:57005/1‑148 (MQ=255)
                    acaTGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTcaaatg                                                                                                              >  4:112901/1‑149 (MQ=255)
                         gTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCg                                                                                                                        <  5:110571/134‑1 (MQ=255)
                         gTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCg                                                                                                                        >  6:110571/1‑134 (MQ=255)
                                    cGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATa                                                                                              <  5:57005/149‑1 (MQ=255)
                                       aaaTTCAGCCTGGTGTCGAAAAATTTTTTAATGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg                                                                                           <  2:152932/149‑1 (MQ=255)
                                       aaaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTcc                                                                                                                          >  1:87666/1‑118 (MQ=255)
                                       aaaTTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTcc                                                                                                                          <  2:87666/118‑1 (MQ=255)
                                                cTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCAccc                                                                                   >  1:167693/1‑148 (MQ=255)
                                                 tGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCTGTTTTCAAATGCAAATGACATTCGATa                                                                                              >  1:182011/1‑136 (MQ=255)
                                                 tGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCTGTTTTCAAATGCAAATGACATTCGATa                                                                                              <  2:182011/136‑1 (MQ=255)
                                                   gtgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAtc                                                                               >  6:148969/1‑149 (MQ=255)
                                                   gtgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAt                                                                                <  5:91521/148‑1 (MQ=255)
                                                    tgtCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAtct                                                                              >  8:84020/1‑149 (MQ=255)
                                                      tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg                                                                                           >  2:79601/1‑134 (MQ=255)
                                                      tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACg                                                                                           <  1:79601/134‑1 (MQ=255)
                                                      tCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGAtctc                                                                             <  7:84020/148‑1 (MQ=255)
                                                         aaaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACAtt                                                                                                   >  7:66636/1‑123 (MQ=255)
                                                         aaaaaTTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACAtt                                                                                                   <  8:66636/123‑1 (MQ=255)
                                                              tttATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAg                                                                    >  5:31547/1‑149 (MQ=255)
                                                                tattaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgc                                                                   >  5:118933/1‑148 (MQ=255)
                                                                  ttaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccag                                                                <  5:148969/149‑1 (MQ=255)
                                                                  ttaAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgcca                                                                 <  2:167693/148‑1 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtttt                                                                                                                                  <  5:34752/81‑1 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCtttt                                                                                                                                  >  6:34752/1‑81 (MQ=255)
                                                                     aaGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCTAgccaggc                                                              >  1:173867/1‑148 (MQ=255)
                                                                       gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         >  2:179350/1‑119 (MQ=255)
                                                                       gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         <  1:179350/119‑1 (MQ=255)
                                                                       gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         <  1:182068/119‑1 (MQ=255)
                                                                       gATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         >  2:182068/1‑119 (MQ=255)
                                                                                    cTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTAttt                                               <  5:132263/148‑1 (MQ=255)
                                                                                    cTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTg                                              <  1:79830/149‑1 (MQ=255)
                                                                                     tAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                              >  3:8380/1‑132 (MQ=255)
                                                                                     tAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAgccaggc                                                              <  4:8380/132‑1 (MQ=255)
                                                                                      aaaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         <  1:116679/104‑1 (MQ=255)
                                                                                      aaaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         >  2:113951/1‑104 (MQ=255)
                                                                                      aaaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGt                                                                                         >  2:116679/1‑104 (MQ=255)
                                                                                      aaaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAt                                            >  4:23681/1‑149 (MQ=255)
                                                                                       aaaaGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGa                                             <  6:31547/147‑1 (MQ=255)
                                                                                          agaaagGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCTAGCCAGGCCAGAAAGTTGTATTTGATGTgg                                        <  2:173867/149‑1 (MQ=255)
                                                                                              atgttaaCATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGt                                    <  3:141188/144‑1 (MQ=255)
                                                                                                 tGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGa                                 <  3:112901/149‑1 (MQ=255)
                                                                                                   aaCATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAtg                                           >  1:150945/1‑137 (MQ=255)
                                                                                                   aaCATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGAtg                                           <  2:150945/137‑1 (MQ=255)
                                                                                                      aTGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACg                            <  3:23681/149‑1 (MQ=255)
                                                                                                         ccTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTATTAATTCCCGTTTTCAAATGCAAATGAc                                                                                                      >  4:76973/1‑72 (MQ=255)
                                                                                                         ccTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTATTAATTCCCGTTTTCAAATGCAAATGAc                                                                                                      <  3:76973/72‑1 (MQ=255)
                                                                                                           tACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCAc                             <  8:50650/143‑1 (MQ=255)
                                                                                                           tACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCAc                             >  7:50650/1‑143 (MQ=255)
                                                                                                           tACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCg                       >  3:104351/1‑149 (MQ=255)
                                                                                                              gATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGt                      >  1:195336/1‑147 (MQ=255)
                                                                                                              gATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGt                      <  2:195336/147‑1 (MQ=255)
                                                                                                                  tGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTGTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGccc                 <  4:104351/148‑1 (MQ=255)
                                                                                                                      gTTAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATca             >  2:94808/1‑148 (MQ=255)
                                                                                                                        tAAGTTATCAAAATTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACaa          >  2:8237/1‑149 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACaa          >  6:94612/1‑140 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACaa          <  5:94612/140‑1 (MQ=255)
                                                                                                                                 aaaaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATg  <  1:94808/148‑1 (MQ=255)
                                                                                                                                   aaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGt       >  6:39571/1‑141 (MQ=255)
                                                                                                                                   aaTTTTGACAAAGGCTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGt       <  5:39571/141‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                   
CCGTAATCTGGGGTGGTGGCACATGGTACACAGGTACGCAAATTCAGCCTGGTGTCGAAAAATTTATTAAAGATTTTAACGATGCTAAAAAGAAAGGTGAACATGCCTACGATATGACGTTAAGTTATCAAAATTTTGACAAAGGCTTTTTTAATTCCCGTTTTCAAATGCAAATGACATTCGATAACGGTGCACCCGATCTCAATATCAAGCCAGGCCAGAAAGTTGTATTTGATGTGGATGTTGAGCACGGTCCGTTGCCCATCACAATGTTAATG  >  NC_000913/4044239‑4044516

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: