Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,628,195 A→G Y294C (TAC→TGC)  nadR → trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,628,1950AG99.2% 412.9 / ‑4.7 119Y294C (TAC→TGC) nadRtrifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (63/54);  total (65/54)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.16e-01

Only 100 of 119 total aligned reads displayed.

CACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGT  >  NC_000913/4628049‑4628339
                                                                                                                                                  |                                                                                                                                                
cACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCa                                                                                                                                                <  2:387624/149‑1 (MQ=255)
 aCCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCAt                                                                                                                                               <  3:259939/149‑1 (MQ=255)
   ccTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCAtt                                                                                                                                              >  8:59702/1‑148 (MQ=255)
   ccTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCAtt                                                                                                                                              >  3:262487/1‑148 (MQ=255)
   ccTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGAGGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCCCGCTCAATGCAtt                                                                                                                                              >  4:106524/1‑148 (MQ=255)
   acTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGTAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTg                                                                                                                                             <  8:196430/148‑1 (MQ=255)
     tGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCAAACCTAGGCGGTGATGAGCTCGCATGGCAGTATTATTACTACGATAACATCGCGCTGGGCCACGCTCAATGCAATGAt                                                                                                                                           >  4:78867/1‑149 (MQ=255)
       gTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtt                                                                                                                                          <  5:244606/148‑1 (MQ=255)
          aacaaacTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTg                                                                                                                                       <  8:492140/148‑1 (MQ=255)
           acaaacTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTACACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCg                                                                                                                                     >  4:135902/1‑149 (MQ=255)
              aacTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATTTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGt                                                                                                                                   >  5:263615/1‑148 (MQ=255)
               acTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCAt                                                                                                                                               >  4:109129/1‑135 (MQ=255)
               acTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCAt                                                                                                                                               <  3:109129/135‑1 (MQ=255)
                      gatatCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGc                                                                                                                          >  2:402994/2‑149 (MQ=255)
                       atatCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGc                                                                                                                          >  6:333502/1‑148 (MQ=255)
                        tatCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCa                                                                                                                        <  4:262487/149‑1 (MQ=255)
                            ttCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATa                                                                                                                     >  5:81289/1‑148 (MQ=255)
                            ttCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATa                                                                                                                     >  5:73503/1‑148 (MQ=255)
                             tCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATa                                                                                                                     >  8:58026/1‑147 (MQ=255)
                                actccaccaGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCTCTCCTCTGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTg                                                                                                                <  6:263615/146‑1 (MQ=255)
                                acacCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTg                                                                                                                <  7:244459/149‑1 (MQ=255)
                                acacCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGAGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTg                                                                                                                >  2:72377/1‑149 (MQ=255)
                                    caccaGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCgg                                                                                                                                    >  6:267626/1‑125 (MQ=255)
                                    caccaGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCAt                                                                                                            >  1:505291/1‑149 (MQ=255)
                                      ccaGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGc                                                                                                                          <  3:403300/133‑1 (MQ=255)
                                      ccaGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGc                                                                                                                          >  4:403300/1‑133 (MQ=255)
                                       caGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTa                                                                                                         >  4:496151/1‑149 (MQ=255)
                                            cGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGa                                                                                                     <  5:333502/148‑1 (MQ=255)
                                            cGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGa                                                                                                     <  7:59702/148‑1 (MQ=255)
                                                     atGGCCGCGATTAAGTCTTTTCACACCTCGGCGGTGCTGAGATCGCATTTCAGTTTTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATTCCAATAAAGTGGCATTTATCGATACCGAtttt                                                                                           <  3:106524/149‑1 (MQ=255)
                                                      tGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAtttt                                                                                           <  6:73503/148‑1 (MQ=255)
                                                      tGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAtttt                                                                                           <  6:81289/148‑1 (MQ=255)
                                                      tGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAt                                                                                              >  4:85554/1‑145 (MQ=255)
                                                      tGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAt                                                                                              <  3:85554/145‑1 (MQ=255)
                                                         ccGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACACTCAATGCATTGATTTTGCGGt                                                                                                                                   >  6:210674/1‑105 (MQ=255)
                                                         ccGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACACTCAATGCATTGATTTTGCGGt                                                                                                                                   <  5:210674/105‑1 (MQ=255)
                                                          cgcgATTATGTCTTTTCACACCTCGGCGGTGACGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtt                                                                                                                                          <  1:140963/97‑1 (MQ=255)
                                                          cgcgATTATGTCTTTTCACACCTCGGCGGTGACGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGAtt                                                                                                                                          >  2:140963/1‑97 (MQ=255)
                                                          cgcgATTATGTCTTTT‑‑CACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTcacca                                                                                    >  4:520672/1‑149 (MQ=255)
                                                           gcgATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATCCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTcacc                                                                                     >  8:255054/1‑149 (MQ=255)
                                                                tATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTca                                                                                       >  3:90432/1‑142 (MQ=255)
                                                                tATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGACAATAAAGTGGCATTTATCGATACCGATTTTGTCACAACt                                                                                  >  7:519864/1‑147 (MQ=255)
                                                                  tGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAtttt                                                                                           >  6:225069/1‑136 (MQ=255)
                                                                  tGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGAtttt                                                                                           <  5:225069/136‑1 (MQ=255)
                                                                   gTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATTGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGc                                                                             >  2:395523/1‑149 (MQ=255)
                                                                   gTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGc                                                                             >  8:185206/1‑149 (MQ=255)
                                                                    tCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGc                                                                             <  3:408985/148‑1 (MQ=255)
                                                                      ttttCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTc                                                                         >  6:156097/1‑149 (MQ=255)
                                                                        ttCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGt                                                                           <  3:324809/146‑1 (MQ=255)
                                                                         tCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCt                                                                        <  5:418594/148‑1 (MQ=255)
                                                                              ccTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCaaa                                                                   >  7:565799/1‑148 (MQ=255)
                                                                                tCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCAAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCaaa                                                                   >  8:551645/1‑146 (MQ=255)
                                                                                      gTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGaa                                                          <  2:63036/149‑1 (MQ=255)
                                                                                       tgatgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAg                                                         <  4:309039/149‑1 (MQ=255)
                                                                                          tgaGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAggg                                                       <  7:185206/148‑1 (MQ=255)
                                                                                           gagaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCg                                                     >  8:527913/1‑149 (MQ=255)
                                                                                            agaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                                                    <  2:505291/149‑1 (MQ=255)
                                                                                            agaTCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCg                                                     <  7:551645/148‑1 (MQ=255)
                                                                                                   aTTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGTAACATc                                             >  2:168752/1‑149 (MQ=255)
                                                                                                    ttGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATcc                                            >  4:287536/1‑149 (MQ=255)
                                                                                                      gCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCgg                                                    >  5:336419/1‑139 (MQ=255)
                                                                                                       cAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGtt                                         >  5:47352/1‑149 (MQ=255)
                                                                                                        aGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTcacca                                                                                    <  8:436595/105‑1 (MQ=255)
                                                                                                        aGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTc                                        <  3:104545/149‑1 (MQ=255)
                                                                                                        aGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTc                                        <  7:58026/149‑1 (MQ=255)
                                                                                                         gTATTCTGACTACGATAAAATCTCGCTGGGCCACGCTCAATGCATTGATTTTTCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAATGGCGGGAACATCCGTTCg                                       <  1:72377/149‑1 (MQ=255)
                                                                                                          tATTCTGACTACGATAAAATTGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGt                                      <  1:395523/149‑1 (MQ=255)
                                                                                                          tATTCTGACTACGATAAAATCGCGCTGGGCCACGCGCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGGGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTGCgg                                      <  6:105184/149‑2 (MQ=255)
                                                                                                            ttCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTg                                     >  4:172876/1‑148 (MQ=255)
                                                                                                               tGACTACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAgg                                 >  3:491319/1‑149 (MQ=255)
                                                                                                                   tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                                             >  2:93016/1‑117 (MQ=255)
                                                                                                                   tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTAc                                                             <  1:93016/117‑1 (MQ=255)
                                                                                                                   tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCt                             >  8:105774/1‑149 (MQ=255)
                                                                                                                   tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCt                             >  3:4321/1‑149 (MQ=255)
                                                                                                                   tACGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCt                             >  4:317237/1‑149 (MQ=255)
                                                                                                                     cGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGt                                          <  2:324741/134‑1 (MQ=255)
                                                                                                                     cGATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGt                                          >  1:324741/1‑134 (MQ=255)
                                                                                                                      gATAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgat                          >  1:301644/1‑149 (MQ=255)
                                                                                                                       aTAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgatt                         <  3:496151/149‑1 (MQ=255)
                                                                                                                        tAAAATCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGACAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTAAGGCGTTCTGCAAAAAGTAAGAAGGGCGTGAACATCCGTTCTTGAAGGCGCttatt                         >  5:434320/1‑148 (MQ=255)
                                                                                                                        tAAAATCGCGCTCGGCCACGCTCAATGCATTGATTTTGCGGTGAAATAAGCCAATAAAGTGGCATTTAACGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgatt                         >  8:224073/1‑148 (MQ=255)
                                                                                                                          aaaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCTGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattga                       <  1:402994/148‑1 (MQ=255)
                                                                                                                          aaaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattga                       <  3:287536/148‑1 (MQ=255)
                                                                                                                           gaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtga                           <  6:534865/142‑1 (MQ=255)
                                                                                                                           aaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattgat                      >  8:549788/1‑148 (MQ=255)
                                                                                                                           aaTCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGAAGGCGCtgataga                       >  5:534865/1‑147 (MQ=255)
                                                                                                                           aaTCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCtgattgat                      >  8:60802/1‑148 (MQ=255)
                                                                                                                             tCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGACCATCCGTTCGTGCAGGCGCTGATTGATGaa                   >  2:130053/1‑149 (MQ=255)
                                                                                                                             tCGCGCTGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGaa                   >  7:435457/1‑149 (MQ=255)
                                                                                                                               gcgcTTGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAAt                  <  7:324036/148‑1 (MQ=255)
                                                                                                                                   tGGGCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGt             >  8:57883/1‑149 (MQ=255)
                                                                                                                                   tGGGACACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGt             >  7:186164/1‑149 (MQ=255)
                                                                                                                                   ggggCCACTCTAAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGt             <  3:78867/148‑1 (MQ=255)
                                                                                                                                     ggCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGttt           <  8:83431/149‑1 (MQ=255)
                                                                                                                                     ggCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGtt            <  7:527913/148‑1 (MQ=255)
                                                                                                                                      gCCACGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTc          >  6:460060/1‑149 (MQ=255)
                                                                                                                                       ccACGCTCAATGCATTGATTTTGCGCTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTAACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGCAACATCCGATCGTGCAGGCGCTGATTGAAGAAAACAGttt           >  5:181297/1‑147 (MQ=255)
                                                                                                                                          cGCTCAATGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATc      >  6:132553/1‑149 (MQ=255)
                                                                                                                                               aaTGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTgg   <  6:47352/147‑1 (MQ=255)
                                                                                                                                               aaTGCATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGt  <  3:317237/148‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CACCCTGGTAAACAAACTTGCCAATATCTTCAACACCACCAGTGCGTGGGAATATGGCCGCGATTATGTCTTTTCACACCTCGGCGGTGATGAGATCGCATTGCAGTATTCTGACTACGATAAAATCGCGCTGGGCCACGCTCAATACATTGATTTTGCGGTGAAATATGCCAATAAAGTGGCATTTATCGATACCGATTTTGTCACCACTCAGGCGTTCTGCAAAAAGTACGAAGGGCGGGAACATCCGTTCGTGCAGGCGCTGATTGATGAATACCGTTTCGATCTGGT  >  NC_000913/4628049‑4628339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: