Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,451,416 C→A 5.4% G90V (GGC→GTC)  rplW ← 50S ribosomal subunit protein L23

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,451,4160CA 5.4% 363.8 / 2.3 112G90V (GGC→GTC) rplW50S ribosomal subunit protein L23
Reads supporting (aligned to +/- strand):  ref base C (57/47);  new base A (3/4);  total (60/52)
Fisher's exact test for biased strand distribution p-value = 7.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGATTTCTGC  >  NC_000913/3451268‑3451564
                                                                                                                                                    |                                                                                                                                                    
 cGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGcc                                                                                                                                                     <  3:229588/149‑1 (MQ=255)
   gAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCctt                                                                                                                                                   >  7:301019/1‑149 (MQ=255)
   gAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCctt                                                                                                                                                   >  4:282064/1‑149 (MQ=255)
    aGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCcttc                                                                                                                                                  <  1:58737/149‑1 (MQ=255)
     gCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTGAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAGCGAAGTCCAGATTCTGGActtcg                                                                                                                                                 <  5:310130/149‑2 (MQ=255)
      cAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCcttctt                                                                                                                                                >  5:114131/1‑149 (MQ=255)
       aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                               >  6:309507/1‑149 (MQ=255)
       aaaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                               >  7:87384/1‑149 (MQ=255)
        aaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGCCTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                               >  2:233340/1‑148 (MQ=255)
        aaaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                               >  2:132894/1‑148 (MQ=255)
         aaGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCttt                                                                                                                                               >  5:43551/1‑147 (MQ=255)
          aGGTTTGCCCTTGTGAATCTCACGTTAAACCACGTTAACTACGTGGCGACGACCCGGAGAGGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAg                                                                                                                                            <  2:111979/149‑1 (MQ=255)
           ggTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAgg                                                                                                                                           <  7:310045/149‑1 (MQ=255)
                   cTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTGTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGt                                                                                                                                    >  8:296874/1‑148 (MQ=255)
                    ttGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGCTTCTGGCCTTCTTTCAGGGTGACGt                                                                                                                                    >  5:13986/1‑147 (MQ=255)
                        gCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCGTTCTTTCAGGGTGACGTAAGCtt                                                                                                                              >  1:344102/1‑149 (MQ=255)
                          aGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttt                                                                                                                            <  7:212151/149‑1 (MQ=255)
                            cTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCtttttt                                                                                                                          <  1:335455/149‑1 (MQ=255)
                               aGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAATGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                                       <  7:264191/149‑1 (MQ=255)
                               aGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCa                                                                                                                       <  2:26432/149‑1 (MQ=255)
                                      aCCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAATCTTTTTTCCAGTCGCTa                                                                                                                <  6:298662/149‑1 (MQ=255)
                                       ccACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTac                                                                                                               >  3:291234/1‑149 (MQ=255)
                                          cTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCa                                                                                                                                             >  5:77964/1‑116 (MQ=255)
                                           tttAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCa                                                                                                                                             <  1:142980/115‑1 (MQ=255)
                                           tttAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCa                                                                                                                                             >  2:142980/1‑115 (MQ=255)
                                             tAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTAcgacga                                                                                                          <  8:325610/148‑1 (MQ=255)
                                              tccTACGTGGCGACGACCCGGAGATGGCGGTTTAAATTTTACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACgacgac                                                                                                         <  6:51799/146‑1 (MQ=255)
                                               aCTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGAcc                                                                                                        >  7:199003/1‑148 (MQ=255)
                                                cTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGa                                                                                                      >  2:31139/1‑149 (MQ=255)
                                                 tACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGa                                                                                                      >  7:316918/1‑148 (MQ=255)
                                                   cGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACACCTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATAc                                                                                                   >  4:206162/1‑149 (MQ=255)
                                                   cGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATAc                                                                                                   <  8:234446/149‑1 (MQ=255)
                                                    gTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCa                                                                                                                                             <  4:217247/106‑1 (MQ=255)
                                                      ggCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCt                                                                                                >  6:85235/1‑149 (MQ=255)
                                                                 ggAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGt                                                                                     >  4:65148/1‑149 (MQ=255)
                                                                  gagaTGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGtt                                                                                    <  3:67020/149‑1 (MQ=255)
                                                                      tGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAAc                                                                                <  4:321912/149‑1 (MQ=255)
                                                                        tCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACtt                                                                              >  2:168828/1‑149 (MQ=255)
                                                                         cGGTTTACATTTAACAACTTCGATTTTATTACTCCTCGGACTTACTCAGCGCCGCCATCGAAGTCCAGATTCTGGCCTTCTTTCAGTGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACttt                                                                             <  8:314499/149‑1 (MQ=255)
                                                                         cGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACttt                                                                             >  7:254810/1‑149 (MQ=255)
                                                                          ggTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACttt                                                                             <  4:291234/148‑1 (MQ=255)
                                                                           gTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTcc                                                                           >  6:185108/1‑149 (MQ=255)
                                                                           gTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCAAGATTCTGGCCTTCTTTCAGTGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTcc                                                                           >  3:40728/1‑149 (MQ=255)
                                                                            tttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTccc                                                                          <  8:310505/149‑1 (MQ=255)
                                                                             ttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTaa                                                                                 >  3:250361/1‑141 (MQ=255)
                                                                             ttACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTaa                                                                                 <  4:250361/141‑1 (MQ=255)
                                                                              tACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCATCGAAGTCCAGATTCTGGGCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCt                                                                         <  6:88488/148‑1 (MQ=255)
                                                                               aCATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCtt                                                                        >  8:325663/1‑148 (MQ=255)
                                                                                cATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTGTTTCCAGTCGCTACGACGAACGAGACGCTGTCCGTGACGTTTAACTTTCCCgtt                                                                       >  6:60971/1‑148 (MQ=255)
                                                                                 aTTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                                     <  2:292998/149‑1 (MQ=255)
                                                                                         acTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAgg                                                             >  6:40345/1‑149 (MQ=255)
                                                                                          cTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGGTTAACTTTCCCTTTAACTTCCAggg                                                            >  1:149662/1‑149 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                         >  6:17379/1‑149 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                         >  6:119961/1‑149 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtg                                                          <  3:210654/148‑1 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgt                                                           <  6:114131/147‑1 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgt                                                           <  1:132894/147‑1 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgt                                                           <  3:282064/147‑1 (MQ=255)
                                                                                             ccATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgt                                                           <  1:168828/147‑1 (MQ=255)
                                                                                              cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                         >  5:303288/1‑148 (MQ=255)
                                                                                              cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                         >  7:212231/1‑148 (MQ=255)
                                                                                              cATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCAGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGgtgt                                                         >  7:171405/1‑148 (MQ=255)
                                                                                               aTTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGCCGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGtt                                                        >  4:49418/1‑148 (MQ=255)
                                                                                               aTTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGtt                                                        >  3:131154/1‑148 (MQ=255)
                                                                                               aTTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGtt                                                        >  7:112050/1‑148 (MQ=255)
                                                                                                ttGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGGGACGTAAGCGTTTTTCCAGTCGCTACGACGACCGATACGCTGGCCGTGCCGTTTAACTTTCCCTTTAACTACCAGGGTGTTaa                                                      <  5:309507/149‑1 (MQ=255)
                                                                                                 tGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAc                                                     >  2:143403/1‑149 (MQ=255)
                                                                                                    aTTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgac                                                  >  1:272702/1‑149 (MQ=255)
                                                                                                        ctcctcCGACTTACTCAGCGCCTCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttc                                               <  7:296874/148‑1 (MQ=255)
                                                                                                         tcctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcga                                             >  4:59484/1‑149 (MQ=255)
                                                                                                         tccaccGACATACTCAGCGCCGCCAACGAAGTCCAGATACTGGCCTTCTTTAAGCCTCACGAAAGCTATTTTCAAGTCGCTACGACGACCGATACGCTGACCCTGACGATTAACTTACCATATAACTACCAGGGTGTTAAagagatcg                                              >  6:199487/1‑143 (MQ=255)
                                                                                                           ctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgact                                           >  1:317674/1‑149 (MQ=255)
                                                                                                           ctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgact                                           >  8:117513/1‑149 (MQ=255)
                                                                                                           ctccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcga                                             <  3:231356/147‑1 (MQ=255)
                                                                                                            tccGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTACCTTTAACTACCAGGGTGTTAAcgacttcgactt                                          >  4:154366/1‑149 (MQ=255)
                                                                                                              cGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgactt                                          <  2:279953/147‑1 (MQ=255)
                                                                                                                aCTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAAcgacttcgacttcgac                                      <  1:31139/149‑1 (MQ=255)
                                                                                                                 cTTACTCATCGCCGCCAACGAATTACAGATTCTGGACTTCTTTCAGGTTGACGTAAGCTTTTTTCCAGTCGCTAAGACTACCGATACGCTGTACGTGACGTTTAACTTTCCCTTTAACTACCATGGTTTTAAcgacttcgacttcgact                                     <  8:199003/149‑1 (MQ=255)
                                                                                                                  ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                                     <  3:248641/116‑1 (MQ=255)
                                                                                                                  ttACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTaa                                                                     >  4:248641/1‑116 (MQ=255)
                                                                                                                      tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                                >  1:247174/1‑149 (MQ=255)
                                                                                                                      tCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                                >  2:242428/1‑149 (MQ=255)
                                                                                                                        aGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                                <  6:43551/147‑1 (MQ=255)
                                                                                                                        aGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCaaa                                <  8:112050/147‑1 (MQ=255)
                                                                                                                        aGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACa                              <  6:303288/149‑1 (MQ=255)
                                                                                                                           gccgccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGtt                           <  5:85235/149‑1 (MQ=255)
                                                                                                                              gccAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCt                        <  1:12129/149‑1 (MQ=255)
                                                                                                                               tgaaagAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTg                       >  1:245330/6‑149 (MQ=255)
                                                                                                                                gaaagAAGGCCAGAATCTGGACTACTTTCAGGGTGACCTAAGCTTTTTTCCAGTCGCTACGACCACCGATACGCTCTCCGTTACCTTTAACTTTACAATTAACTACAAGTCTGTTAACGACTACAACTTCGACTGCAAACAGTTTCTg                       >  4:203920/5‑148 (MQ=255)
                                                                                                                                cAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGc                      >  3:272344/1‑149 (MQ=255)
                                                                                                                                  aagAAGGCCAGAATCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGca                     >  7:59803/3‑148 (MQ=255)
                                                                                                                                   cGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGcaca                   <  5:119961/149‑1 (MQ=255)
                                                                                                                                        tCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGCCTTCGACTTCGACTTCAAACAGTTTCTGCACagcagc              >  7:189008/1‑149 (MQ=255)
                                                                                                                                          cAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCtt            >  4:280698/1‑149 (MQ=255)
                                                                                                                                           aGATTCTGGCCTTCTTTCAGGGTGACGTAAGGTTTTTTCCTGTCGCTACGACGACCGATACGCTGTCCGTGACGTATAACTATCCCTATAACTACCAGCGTGTTAACTCCTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCtat           >  4:253058/1‑147 (MQ=255)
                                                                                                                                              ttCTTGCCTTATTTCAGGGTGACTTAAGCTTTTTTCCAGTCTCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGAt        <  6:13986/149‑1 (MQ=255)
                                                                                                                                              atCTGGACTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGGGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTTAt        <  3:203920/148‑1 (MQ=255)
                                                                                                                                                 tGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTCCAAACAGTTTCTGCACAGCAGCTTTGATTTc     >  8:43746/1‑149 (MQ=255)
                                                                                                                                                   gCCTTCTTTCAGGGGGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCGTTGAttt      <  6:47957/146‑1 (MQ=255)
                                                                                                                                                    ccTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGATTTCTGc  >  4:285877/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ACGGAGCAAAAGGTTTGCCCTTGTGCAGCTCAGGGTTAACCACTTTAACTACGTGGCGACGACCCGGAGATGTCGGTTTACATTTAACAACTGCCATTGTATTACTCCTCCGACTTACTCAGCGCCGCCAACGAAGTCCAGATTCTGGCCTTCTTTCAGGGTGACGTAAGCTTTTTTCCAGTCGCTACGACGACCGATACGCTGTCCGTGACGTTTAACTTTCCCTTTAACTACCAGGGTGTTAACGACTTCGACTTCGACTTCAAACAGTTTCTGCACAGCAGCTTTGATTTCTGC  >  NC_000913/3451268‑3451564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: