| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 2,355,891 | T→C | 7.1% | L124P (CTG→CCG) | glpC → | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,355,891 | 0 | T | C | 7.1% | 166.6 / 3.4 | 56 | L124P (CTG→CCG) | glpC | anaerobic sn‑glycerol‑3‑phosphate dehydrogenase, C subunit, 4Fe‑4S iron‑sulfur cluster |
| Reads supporting (aligned to +/- strand): ref base T (21/31); new base C (2/2); total (23/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.76e-01 | |||||||||||
TATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAAC > NC_000913/2355745‑2356036 | tatCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAgctgc > 2:269180/1‑149 (MQ=255) cAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGa < 6:66075/149‑1 (MQ=255) cAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGa < 2:98847/149‑1 (MQ=255) gcgcgcgcGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATg < 4:144137/149‑1 (MQ=255) cgcgcgctGGCGAAATATGACCACACGCGCCCGTCGCTGCGTAATTTTTTGTGGAGTCATACCGACCTTCGGGGTAGCGTTTCCACGCCGGTCTCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATgc < 2:197741/149‑1 (MQ=255) gcgcgcgGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATgcg < 1:300761/149‑1 (MQ=255) aaaTATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTaaaaa > 6:206464/1‑148 (MQ=255) aaaTATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAAt < 5:301489/149‑1 (MQ=255) aaaTATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAAt < 1:269180/149‑1 (MQ=255) tGACACCCCGCTCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATAGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGGGGCGTTAAAAATCGat < 7:246680/148‑1 (MQ=255) acacCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGatca < 3:215674/148‑1 (MQ=255) caccccGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCCTACCGAACTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGatcatc < 1:97170/149‑1 (MQ=255) accacGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcg > 2:226992/1‑149 (MQ=255) ccacGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcgc > 2:307398/1‑149 (MQ=255) acGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATcgccg > 7:265339/1‑149 (MQ=255) cgcCCGGCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCAc < 1:276319/149‑1 (MQ=255) gTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACTCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTAc < 6:43902/149‑1 (MQ=255) tGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGaaa < 7:163837/149‑1 (MQ=255) tGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGaaa > 3:186620/1‑149 (MQ=255) agCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGaaa > 1:175746/2‑149 (MQ=255) cGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGaaa < 4:186620/147‑1 (MQ=255) tgtgTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGGCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTc < 4:254930/148‑1 (MQ=255) ttGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCAc > 8:104367/1‑149 (MQ=255) ttGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCAc > 4:87745/1‑149 (MQ=255) ttGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCAc > 8:280502/1‑149 (MQ=255) aTACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGt < 4:154181/149‑1 (MQ=255) cGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCt > 1:273948/1‑149 (MQ=255) gACCTGATGGGTAGCGTTTCCACGCCGTTCTCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTg < 5:84579/149‑1 (MQ=255) ccTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGt < 8:183862/149‑1 (MQ=255) tGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTAt > 6:191329/1‑149 (MQ=255) aTGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTAt < 5:206464/147‑1 (MQ=255) ggTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAg > 8:38032/1‑149 (MQ=255) gCGTTTCCACGCCGTTCGCACCACTCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCTGCTGCTTGATGCGGCGGTAAAACTCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTg < 7:208145/149‑1 (MQ=255) gCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTg < 7:208144/149‑1 (MQ=255) cGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTgg < 2:243474/149‑1 (MQ=255) gTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAACCCGGTGCGGCCGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTCCCGAAATACTCCTTCGGCGCGTTCCGTCGCTGGTATCGCCGCGTggc > 5:336798/1‑149 (MQ=255) tCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTggcgg < 4:28929/148‑1 (MQ=255) cGTTCGCACCAATCGTCAACACCGCTACCTCGCTGATACCGTTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCAAGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaa < 2:175746/149‑1 (MQ=255) gTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaag > 6:99000/1‑149 (MQ=255) gTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCagcaag < 1:60540/149‑1 (MQ=255) gCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCCGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGcac > 6:131088/1‑148 (MQ=255) cACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACaa < 3:87745/149‑1 (MQ=255) tCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGAc > 4:75346/1‑149 (MQ=255) acacCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAg > 8:24933/1‑149 (MQ=255) cGCTACCTCGCTGAAACCGGTGCGGCAGCCGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCg > 2:139105/1‑149 (MQ=255) gCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCAAGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGc > 6:130610/1‑149 (MQ=255) cGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGctttcttt > 5:257776/1‑149 (MQ=255) gCTGAAACCGGTGCGGCAGCCGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCtttctttc < 1:139105/149‑1 (MQ=255) aaaCCGGTGCGGCAGCCGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCAc < 5:131088/147‑1 (MQ=255) aCCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGgct < 7:280502/149‑1 (MQ=255) aCCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGgct < 7:38032/149‑1 (MQ=255) tGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGGCGCTTTCTTTCACGgctgctgc < 1:282728/149‑3 (MQ=255) cGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCg < 7:24933/148‑1 (MQ=255) ggCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGt > 7:176906/1‑148 (MQ=255) gCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGtt > 7:165664/1‑148 (MQ=255) aGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTg > 7:206872/1‑76 (MQ=255) aGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTg < 8:206872/76‑1 (MQ=255) gctgctTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGACGCTTTCTTTCACGGCTGCTTCGTTAAc > 3:104414/1‑148 (MQ=255) | TATCCAGCGCGCGCGGGCGAAATATGACACCACGCGCCCGTCGCTGCGTAATTTTGTGTTGAGTCATACCGACCTGATGGGTAGCGTTTCCACGCCGTTCGCACCAATCGTCAACACCGCTACCTCGCTGAAACCGGTGCGGCAGCTGCTTGATGCGGCGTTAAAAATCGATCATCGCCGCACGCTACCGAAATACTCCTTCGGCACGTTCCGTCGCTGGTATCGCAGCGTGGCGGCTCAGCAAGCACAATATAAAGACCAGGTCGCTTTCTTTCACGGCTGCTTCGTTAAC > NC_000913/2355745‑2356036 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |