Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 342,876 T→C 6.1% intergenic (+369/‑8) yahJ → / → yahK putative metallo‑dependent hydrolase domain deaminase/broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913342,8760TC 6.1% 197.7 / 2.8 67intergenic (+369/‑8)yahJ/yahKputative metallo‑dependent hydrolase domain deaminase/broad specificity NADPH‑dependent aldehyde reductase, Zn‑containing
Reads supporting (aligned to +/- strand):  ref base T (29/33);  new base C (2/2);  total (32/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

ATGGGGAACGTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTC  >  NC_000913/342731‑343024
                                                                                                                                                 |                                                                                                                                                    
aTGGGGAACGTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTaa                                                                                                                                                    <  3:29090/148‑1 (MQ=255)
   gggAACGTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAaca                                                                                                                                                 >  6:282086/1‑148 (MQ=255)
   gggAACGTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAaca                                                                                                                                                 <  2:121371/148‑1 (MQ=255)
    ggAACGTTGCTGCTCAGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGAGTTGCCAAATACACATAGCTAATCAGGAGTAAtcac                                                                                                                                                >  5:70558/1‑148 (MQ=255)
         gTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGaa                                                                                                                                          <  1:151566/149‑1 (MQ=255)
             ctgctCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGAt                                                                                                                                       <  6:253238/148‑1 (MQ=255)
                    gTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTg                                                                                                                               >  2:72356/1‑149 (MQ=255)
                     tttCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCGAAGCTGt                                                                                                                              <  3:232473/149‑1 (MQ=255)
                     tttCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGC‑‑CGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTg                                                                                                                            <  6:19048/149‑1 (MQ=255)
                          ttcaGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTg                                                                                                                         <  8:317633/149‑1 (MQ=255)
                             aGGCTCGCGGCATGTTGGGGCTGGATAGGGTGGTTTAAATATTGGCTATATTCTATGGACGCGTTTTGCCGCGATGACATATCAGGCGATGCAAAATACACATATCTAAACAGTAGTAAACACAATGAAGATCAAAGCTGTTGGTGCa                                                                                                                       >  2:102686/1‑148 (MQ=255)
                              ggCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCata                                                                                                                     >  7:35945/1‑149 (MQ=255)
                                 tCGCGGCATGTACGGGCTGGAGCGGGTGGTATAAATATTGGCTATATTCAATGGACGCGTTTTGCCGAGATGAAATATCAGGCGTTGCCAAAAACACATAGCTAATCAGGAGTACACACAATGAAGATCAAAGCTGTTGGTGCATATTc                                                                                                                  >  8:295761/1‑149 (MQ=255)
                                   gcgGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTc                                                                                                                  <  5:282086/147‑1 (MQ=255)
                                    cgGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGc                                                                                                               <  3:232215/149‑1 (MQ=255)
                                        aTGTTGGGGCTGGATCGGGTGGGTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTaaa                                                                                                           <  3:49325/149‑1 (MQ=255)
                                        aTGTTGGGGCTGGATCGGGGGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTaaa                                                                                                           <  6:149480/149‑1 (MQ=255)
                                                 cTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCATATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAAAAACCACtt                                                                                                  <  2:107781/149‑1 (MQ=255)
                                                  tGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGTAGTTGACAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGAATATTCCGCTAAACAACCACtt                                                                                                  >  2:21934/1‑148 (MQ=255)
                                                   ggATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTg                                                                                                 >  6:119259/1‑148 (MQ=255)
                                                         ggtggtTTAAATATTGGATATATTCAATGGACGAGTTTTGCAGCGATGACATTTCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAAGACAATGAAGATCAAAGCTGGTGGTGCATATTCCGCTAATCGACCCCTTGAAACGAt                                                                                          >  2:28555/1‑149 (MQ=255)
                                                          gtggtTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATg                                                                                         <  5:119259/149‑1 (MQ=255)
                                                             gtTTAATTATTGGCTATATTCAATTGTCGCGTTTTGCCGCGATGACATATAAGGCGTTGCCAAATACACATATCTAATCAGTAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGa                                                                                       <  8:250412/148‑1 (MQ=255)
                                                              tttAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGAGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGata                                                                                     >  5:85992/1‑149 (MQ=255)
                                                               ttAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGatat                                                                                    >  1:165028/1‑149 (MQ=255)
                                                                       tGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGc                                                                            >  2:17828/1‑149 (MQ=255)
                                                                       tGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGc                                                                            >  4:6352/1‑149 (MQ=255)
                                                                         gCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGat                                                                                      >  1:185095/1‑137 (MQ=255)
                                                                         gCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGat                                                                                      <  2:185095/137‑1 (MQ=255)
                                                                             tatTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGCTCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCAGGCGTGacc                                                                      >  3:156343/1‑147 (MQ=255)
                                                                             tatTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAAc                                                                      >  3:314646/1‑149 (MQ=255)
                                                                             tatTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAAc                                                                      >  6:119206/1‑149 (MQ=255)
                                                                               ttCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAAcc                                                                     <  2:331548/148‑1 (MQ=255)
                                                                               ttCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCg                                                                    >  8:223863/1‑149 (MQ=255)
                                                                                  aaTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 <  5:10689/149‑1 (MQ=255)
                                                                                  aaTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 <  4:222192/149‑1 (MQ=255)
                                                                                  aaTGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 >  1:150134/1‑149 (MQ=255)
                                                                                  aaTGGACGCGTGTTGCCGCGATGACATATCATGCGGTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 <  1:327887/149‑1 (MQ=255)
                                                                                      gACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCg                                                              >  4:278254/1‑148 (MQ=255)
                                                                                        cgcgTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAat                                                           >  8:137850/1‑149 (MQ=255)
                                                                                           gTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAatgat                                                        <  7:22542/149‑1 (MQ=255)
                                                                                            ttttGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAatgatg                                                       >  2:346834/1‑149 (MQ=255)
                                                                                                   gcgAAGACATATCAGGCGTTGCCAAATACACATACCTAATCAGGACAAAACACAAACAACATCAAAGCTGATGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCCGTACCGAATGATGTCaaaag                                                >  8:115118/1‑148 (MQ=255)
                                                                                                     gATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGTGTAACCACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTACACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcg                                              <  4:56395/149‑1 (MQ=255)
                                                                                                          cATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaaatc                                         <  7:223863/149‑1 (MQ=255)
                                                                                                           atatCAGGCGTTGCCAAATACACATAGCTAATCAGGAGCAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCTGCGTGAACCGGGACCGAATGATGTCAaaatcgaaatcg                                        >  6:96986/1‑149 (MQ=255)
                                                                                                           atatCAGGCGTTGCCAAATACACATAGCTAATCAGGAGCAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAaaatcgaaatcg                                        >  6:101636/1‑149 (MQ=255)
                                                                                                            tatCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCaaa                                                                                                                                   <  7:244161/57‑1 (MQ=255)
                                                                                                            tatCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCaaa                                                                                                                                   >  8:244161/1‑57 (MQ=255)
                                                                                                            tatCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGc                                       <  2:150134/149‑1 (MQ=255)
                                                                                                                aGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 <  6:22287/119‑1 (MQ=255)
                                                                                                                aGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGa                                                                 >  5:22287/1‑119 (MQ=255)
                                                                                                                aGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTa                                    <  3:110823/148‑1 (MQ=255)
                                                                                                                 ggCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACt                                  >  6:207740/1‑149 (MQ=255)
                                                                                                                          aaaTACCCATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACACCCACTTGAACCGAGGGATATCACCCGGCGGGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGttt                         <  6:157782/149‑1 (MQ=255)
                                                                                                                                    aGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTACGCTAAACAACCACTGGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAAGGATGTCAAACTCGAAATCGCTTACTGTGGCGTTTGCCATGccta               >  6:223900/1‑147 (MQ=255)
                                                                                                                                     gCTAATCAGGCGTACCCACCATGAACATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCTATGGATATCACACGGCTTGAACCGGTACCGAATTATGTCAAAATAGAAATCGCTTACTGTGGCGTTTGCCATTCCGAt              <  6:70558/149‑1 (MQ=255)
                                                                                                                                     gCTAATCAGGAGCAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGa               <  5:101636/148‑1 (MQ=255)
                                                                                                                                     gCTAATCAGGAGCAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGa               <  5:96986/148‑1 (MQ=255)
                                                                                                                                      cTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc             <  8:35945/149‑1 (MQ=255)
                                                                                                                                      cTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc             >  7:10951/1‑149 (MQ=255)
                                                                                                                                      cTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc             <  3:329257/149‑1 (MQ=255)
                                                                                                                                       tAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtct            >  6:255056/1‑149 (MQ=255)
                                                                                                                                         aTCAGTAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGAtc             <  1:21934/146‑1 (MQ=255)
                                                                                                                                            aGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAAACGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTccacc       <  7:295761/149‑1 (MQ=255)
                                                                                                                                             ggAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCGGATCTcaac        >  6:194199/1‑147 (MQ=255)
                                                                                                                                              gAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAg     <  5:207740/149‑1 (MQ=255)
                                                                                                                                                 tAAACACAATGAAGATCAAAGCTGTTTGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTGTGCCATTACGATATCCACCAGGTc  >  5:103379/1‑149 (MQ=255)
                                                                                                                                                 tAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTc  >  2:279239/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
ATGGGGAACGTTGCTGCTCTGTTTCATTCAGGCTCGCGGCATGTTGGGGCTGGATCGGGTGGTTTAAATATTGGCTATATTCAATGGACGCGTTTTGCCGCGATGACATATCAGGCGTTGCCAAATACACATAGCTAATCAGGAGTAAACACAATGAAGATCAAAGCTGTTGGTGCATATTCCGCTAAACAACCACTTGAACCGATGGATATCACCCGGCGTGAACCGGGACCGAATGATGTCAAAATCGAAATCGCTTACTGTGGCGTTTGCCATTCCGATCTCCACCAGGTC  >  NC_000913/342731‑343024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: