Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,854,741 G→A 5.1% R38R (CGC→CGT)  ydjF ← putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,854,7410GA 5.1% 244.2 / 2.2 78R38R (CGC→CGT) ydjFputative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (40/33);  new base A (2/2);  total (43/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.71e-01

TGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTA  >  NC_000913/1854594‑1854887
                                                                                                                                                   |                                                                                                                                                  
tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATcgc                                                                                                                                                   >  2:164934/1‑149 (MQ=255)
tGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATcgc                                                                                                                                                   >  2:158806/1‑149 (MQ=255)
   cTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAATCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGATGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCTTCTTCCAGCTTCTCAAGATCgaggc                                                                                                                                                >  8:294219/1‑149 (MQ=255)
     gcaaggCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGa                                                                                                                                              >  5:337195/6‑149 (MQ=255)
       cGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAAt                                                                                                                                            >  6:72832/1‑149 (MQ=255)
           cAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGTTTCTGGTCAAAAAGCCTTCATCTTCCATCTTCTCAAGATCGCGGCGAATGGtt                                                                                                                                        <  1:164390/149‑1 (MQ=255)
              tAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttct                                                                                                                                     >  7:16350/1‑149 (MQ=255)
              tAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttct                                                                                                                                     >  8:129032/1‑149 (MQ=255)
              tAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttct                                                                                                                                     >  7:60905/1‑149 (MQ=255)
                 gctgctTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCg                                                                                                                                  >  3:173212/1‑149 (MQ=255)
                  ctgctTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCgg                                                                                                                                 >  7:323308/1‑149 (MQ=255)
                      ttCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttcttc                                                                                                                                   >  4:284497/1‑143 (MQ=255)
                      ttCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTttcttc                                                                                                                                   <  3:284497/143‑1 (MQ=255)
                      ttCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTAc                                                                                                                             >  1:185898/1‑149 (MQ=255)
                              aTAAACCGATTAAGCGCGCTTATATAATTTGATATTCTCCTTCAACATCGCTTTATTTAAAACAGCACAACCATATGTTCTGTTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGTTCGCGGCGAATGGTTTCTTCTGTTACCTGaaaaa                                                                                                                     <  2:294586/149‑1 (MQ=255)
                                 aaaCGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAATCAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATcc                                                                                                                  <  8:323308/149‑1 (MQ=255)
                                 aaaCGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATcc                                                                                                                  >  2:147474/1‑149 (MQ=255)
                                 aaaCGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATc                                                                                                                   <  1:164934/148‑1 (MQ=255)
                                 aaaCGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTAATTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCAAGCTTCTCAAGATCGCGGAGAATGCTTTCTTCGGTTACCTGAAAAATcc                                                                                                                  >  8:327198/1‑149 (MQ=255)
                                  aaCGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACAAACATAGGTTCTGGTCAAAAAGCCTTCATCTACCAGCTTCTCAAGATCGCTGAGAATGGTTTCTTAGGTTACCTGAAAAATccc                                                                                                                 >  6:234118/1‑149 (MQ=255)
                                          aGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAt                                                                                                         >  8:347792/1‑149 (MQ=255)
                                             gcgcTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAttt                                                                                                       >  7:192104/1‑148 (MQ=255)
                                              cgcTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTga                                                                                                     <  4:125868/149‑1 (MQ=255)
                                                   ataAAAATGGATATTCTCCGTCAACATCTCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACt                                                                                                 <  2:133555/148‑1 (MQ=255)
                                                   ataAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTCCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACt                                                                                                 <  4:151529/148‑1 (MQ=255)
                                                        aaTGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCAcc                                                                                           <  4:24552/149‑1 (MQ=255)
                                                                     cGTCAACATCGCTGTATTTAAAACAGCACCACGATAGGTTCTGGTCAAAAACCCTTCATCTTCCAGCCTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGtt                                                                                <  7:154417/147‑1 (MQ=255)
                                                                       tCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGACAAAAAGCCTTCATCTTCCAGCTTCTCAAGAACGCGGCGAATGGTTTCTTCGGTGACCTGAAAAATACCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGc                                                                             >  8:48170/1‑148 (MQ=255)
                                                                          aCATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAAcc                                                                         <  6:239641/149‑1 (MQ=255)
                                                                               gCTGTATTTAAAACAGCACCACCATAGGTTCTTGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCAttt                                                                     <  7:327198/148‑1 (MQ=255)
                                                                                cTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGc                                                                   >  6:313353/1‑149 (MQ=255)
                                                                                 tGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCt                                                                  >  6:48683/1‑149 (MQ=255)
                                                                                 tGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCGTCGGTTAACTGAAAAATCCCACTCAAATTTGCGACTGTCACCATTTTATCGTTGGCAACCATTTGca                                                                  >  2:324930/1‑148 (MQ=255)
                                                                                    aTTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTaa                                                               >  2:165785/1‑149 (MQ=255)
                                                                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCTCGGCGAATGGTTTCTTCGGTTACCTGATAAATCCCACTCAAATTTGAGCCTGTCCCCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                              >  4:198762/1‑149 (MQ=255)
                                                                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAttt                                                                                                       <  3:223388/108‑1 (MQ=255)
                                                                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAttt                                                                                                       >  4:223388/1‑108 (MQ=255)
                                                                                     tttAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                              >  8:233093/1‑149 (MQ=255)
                                                                                     tttAAAACAGCACCACCATAGGATCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTAAAGATCGCGGCGAATGGTTTCTTCTGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                              >  5:165727/1‑149 (MQ=255)
                                                                                       tAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAtt                                                             <  7:129032/148‑1 (MQ=255)
                                                                                       tAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAtt                                                             <  2:178752/148‑1 (MQ=255)
                                                                                       tAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                              <  1:112220/147‑1 (MQ=255)
                                                                                       tAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAAt                                                              >  2:112220/1‑147 (MQ=255)
                                                                                                   accaTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGt                                                <  3:61625/149‑1 (MQ=255)
                                                                                                    ccaTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTga                                                                                                     <  1:343008/95‑1 (MQ=255)
                                                                                                    ccaTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTga                                                                                                     >  2:343008/1‑95 (MQ=255)
                                                                                                    ccaTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTc                                               >  7:308494/1‑149 (MQ=255)
                                                                                                      aTAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCtt                                             >  7:181895/1‑149 (MQ=255)
                                                                                                       tAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCttt                                            <  8:60905/149‑1 (MQ=255)
                                                                                                       tAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCttt                                            <  7:347792/149‑1 (MQ=255)
                                                                                                         ggTTCTGTGAAAAAAGCCTTCATCTTCCAGCTTTTCATGAACGCGGCTAATGGTTTTTTCGGTTACCTGAAAAATCCCATTAAAATTTCAGACTGTCACTTTTTTATCGTTGGAAACCATTTGCTTAATTGCTTTAATCGTGTAtttt                                           <  8:16350/148‑1 (MQ=255)
                                                                                                              tGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgccgcc                                     >  2:52497/1‑149 (MQ=255)
                                                                                                              tGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgccgc                                      >  1:293404/1‑148 (MQ=255)
                                                                                                              tGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGAAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTgccgc                                      >  1:298074/1‑148 (MQ=255)
                                                                                                              tGGTCAAAAAGACATCATCTTCCAGCTTCTCAAGATAGCGGCGAATGGTTTCTTAGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCCTTGGCAACCATTTGCATAATTGCATGAATACTGTCTTTTgccgc                                      >  6:230921/1‑148 (MQ=255)
                                                                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGa                                 <  1:103825/148‑1 (MQ=255)
                                                                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                >  1:183891/1‑149 (MQ=255)
                                                                                                                   aaaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAAATTGACACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                <  2:333783/149‑1 (MQ=255)
                                                                                                                    aaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAACTCCCACTCAAATTTGAGACTGTCACCTTTTTAt                                                                                    >  5:154079/1‑96 (MQ=255)
                                                                                                                    aaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTAt                                                                                    <  6:154079/96‑1 (MQ=255)
                                                                                                                    aaaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGAt                                >  6:46899/1‑148 (MQ=255)
                                                                                                                     aaaGCGTTCATCTTCCAGGTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGTAAAATCCGACTGAATTTTGAGTCTGTCACCTTTTTTTCGTTGGCTACCATTTGCTTAATTGCCTTAATCCTGTCTTTTGCCGCCACGATTa                              <  6:243357/149‑1 (MQ=255)
                                                                                                                     aaaGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTCCCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTa                              <  3:85286/149‑1 (MQ=255)
                                                                                                                       aGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTaca                            >  4:111838/1‑149 (MQ=255)
                                                                                                                        gCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGa                                 <  4:277909/143‑1 (MQ=255)
                                                                                                                        gCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGa                                 >  3:277909/1‑143 (MQ=255)
                                                                                                                          cTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAATTTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCGTAATTGCCTGAATCCTGTGTTTTGCCGCCACGATTACAccc                         >  4:91084/1‑149 (MQ=255)
                                                                                                                          cTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACAccc                         <  5:48683/149‑1 (MQ=255)
                                                                                                                          cTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACAccc                         <  5:72832/149‑1 (MQ=255)
                                                                                                                             cATCTTCCAGCTTCTCAAGATCACGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCt                       >  1:9408/1‑148 (MQ=255)
                                                                                                                             cATCTTCCAGCTTCTCAAGATCACGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCt                       >  1:15158/1‑148 (MQ=255)
                                                                                                                             cATCTTCCAGCTTCTCAAGATCACGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCt                       <  2:9408/148‑1 (MQ=255)
                                                                                                                             cATCTTCCAGCTTCTCAAGATCACGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCt                       <  2:15158/148‑1 (MQ=255)
                                                                                                                                   ccAGCTTCTCAAGATCGCGGCGAATGCTATCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCACCGATTACACCCCTGTATCtt                >  6:319084/1‑149 (MQ=255)
                                                                                                                                    cAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACGCCTGTATCttt               >  2:311539/1‑149 (MQ=255)
                                                                                                                                      gCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCttttt             <  3:111838/149‑1 (MQ=255)
                                                                                                                                            cAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATca       >  4:16793/1‑149 (MQ=255)
                                                                                                                                             aaGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACAt         <  8:181895/146‑1 (MQ=255)
                                                                                                                                             aaGATCGCGGCGAATGGTTGCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACAt         <  1:158806/146‑1 (MQ=255)
                                                                                                                                                aTCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTTTCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACAt    >  2:153582/1‑148 (MQ=255)
                                                                                                                                                 tCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTa  <  8:308494/149‑1 (MQ=255)
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TGCCTTGCGTGCAATAAGCTGCTTCTCTTCATAAAACGATGAAGCGCGCTTATAAAAATGGATATTCTCCGTCAACATCGCTGTATTTAAAACAGCACCACCATAGGTTCTGGTCAAAAAGCCTTCATCTTCCAGCTTCTCAAGATCGCGGCGAATGGTTTCTTCGGTTACCTGAAAAATCCCACTCAAATTTGAGACTGTCACCTTTTTATCGTTGGCAACCATTTGCTTAATTGCCTGAATCCTGTCTTTTGCCGCCACGATTACACCCCTGTATCTTTTTACATCACATTA  >  NC_000913/1854594‑1854887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑