Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,811,349 A→G 5.2% Q139R (CAG→CGG)  ydjN → putative transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,811,3490AG 5.2% 237.3 / 2.6 76Q139R (CAG→CGG) ydjNputative transporter
Reads supporting (aligned to +/- strand):  ref base A (35/37);  new base G (2/2);  total (37/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.31e-01

AGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCGACGT  >  NC_000913/1811201‑1811489
                                                                                                                                                    |                                                                                                                                            
aGCGCGGTTGCCCGTCTGCGTAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCa                                                                                                                                              <  8:298240/149‑1 (MQ=255)
aGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCa                                                                                                                                              <  7:173061/149‑1 (MQ=255)
aGCGCGGATGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCa                                                                                                                                              <  4:43738/149‑1 (MQ=255)
  cgcgGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAgg                                                                                                                                            <  1:354135/149‑1 (MQ=255)
  cgcgGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGGGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAgg                                                                                                                                            <  1:157045/149‑1 (MQ=255)
    cgGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGggt                                                                                                                                          >  3:315178/1‑149 (MQ=255)
     ggTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGggtg                                                                                                                                         <  6:161280/149‑1 (MQ=255)
     ggTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGggtg                                                                                                                                         <  7:141204/149‑1 (MQ=255)
          gccGTATGCATTACGCATCTCAGTTAGGCAAATGCAGTTTTCTGACCATCGGTACGCTTTTGTTTACAACGCTGATTTCGGCGCTGGTCGGTGTGCTGGTCAACAACCTGTTTGGTTTTACGGCTGAAGGTCTGGTTCAGGGTGGTGCa                                                                                                                                    <  1:322473/148‑1 (MQ=255)
           ccGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCGGGgtggtg                                                                                                                                      >  7:186279/1‑146 (MQ=255)
           ccGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGgtggtg                                                                                                                                      >  1:119273/1‑146 (MQ=255)
              tCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGaaa                                                                                                                                <  5:242411/149‑1 (MQ=255)
                tGCATAACGCATCTCAGTTAGGCAAAGTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACt                                                                                                                              >  8:43440/1‑149 (MQ=255)
                tGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACt                                                                                                                              >  2:171328/1‑149 (MQ=255)
                  cATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCGGGGTGGTGCAGAAACTg                                                                                                                             >  3:235377/1‑148 (MQ=255)
                  cATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTg                                                                                                                             <  7:300882/148‑1 (MQ=255)
                  cATAACGCATCACAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAAGCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTg                                                                                                                             >  2:341335/1‑148 (MQ=255)
                   aTAACGCATCTCAGTTAGGCAAAGTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCa                                                                                                                           <  7:43440/149‑1 (MQ=255)
                   aTAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGc                                                                                                                            <  5:293796/148‑1 (MQ=255)
                   aTAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGc                                                                                                                            >  6:293796/1‑148 (MQ=255)
                   aTAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCAACAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCa                                                                                                                           >  3:100145/1‑149 (MQ=255)
                    tAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCAc                                                                                                                          <  2:248544/149‑1 (MQ=255)
                               aGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGc                                                                                                                >  7:9859/1‑148 (MQ=255)
                                   aGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCAtt                                                                                                            <  7:78175/148‑1 (MQ=255)
                                   aGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCAtt                                                                                                            <  8:133910/148‑1 (MQ=255)
                                   aGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCAtt                                                                                                            <  1:171328/148‑1 (MQ=255)
                                      cAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGaaa                                                                                                        <  2:119273/149‑1 (MQ=255)
                                       aaaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCt                                                                                                                      <  6:58137/134‑1 (MQ=255)
                                       aaaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCt                                                                                                                      >  5:58137/1‑134 (MQ=255)
                                       aaaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAg                                                                                                       <  8:9859/149‑1 (MQ=255)
                                       aaaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACATGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAg                                                                                                       >  5:119819/1‑149 (MQ=255)
                                        aaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTTACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGCAACAGCACGTCTGCACCCCATTCAAAg                                                                                                       >  2:237529/1‑148 (MQ=255)
                                        aaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAg                                                                                                       >  8:44224/1‑148 (MQ=255)
                                        aaaTCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGt                                                                                                      <  5:331883/149‑1 (MQ=255)
                                                 ttCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGt                                                                                              <  4:100145/148‑1 (MQ=255)
                                                       ccATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCGGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCGGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTaaa                                                                                       <  8:186279/149‑1 (MQ=255)
                                                           cGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGtct                                                                                   >  8:256188/1‑149 (MQ=255)
                                                              tACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTctct                                                                                 >  1:32052/1‑148 (MQ=255)
                                                                cGCTTTTGTTTAGCACTCTGATTGCTGCGATGGTCGTTGTGCTGGTCACCAACCTGTTTGGTTTGACGTCTTAATGTCTGTTTCAGTGTGGTGCAGAAACTGCACGTATGAACGCCATTGAAAGTAACTATGTTGGTAAATTCtcttat                                                                              <  3:304317/149‑1 (MQ=255)
                                                                     ttGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCGGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCtctgatctg                                                                           <  4:235377/147‑1 (MQ=255)
                                                                            ccACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCg                                                                  <  3:220704/149‑1 (MQ=255)
                                                                             cACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGc                                                                 >  1:258302/1‑149 (MQ=255)
                                                                              aCGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTTGTAAAGTCTCTGATCTGAGCGTTCCg                                                                  >  6:107993/1‑147 (MQ=255)
                                                                                  tGATTGCGGCGCTGGTCGGTGTGGTGGTCTCTCACCTGGTTGGTTTGACGGCGGAAGCTGTCATTCAGGCAGGGGCAGTAACTACACGGCTGAACGACATTGATAATAATTATGTTGGAAAAGTCACTGATCTGCGCGTTCCGctgctg                                                            <  4:217484/149‑1 (MQ=255)
                                                                                  tGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTg                                                            >  1:123601/1‑149 (MQ=255)
                                                                                     ttGCGGCGCTGGTCGGTGTGCGGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTc                                                         <  6:32590/149‑1 (MQ=255)
                                                                                     ttGCGGCGCTGGGCGGTGTGCTGGTCACGAACCTGTTTGGTTTGACGGCTTAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGGTCCGCAGCTGGTc                                                         <  5:281963/149‑1 (MQ=255)
                                                                                        cggcgCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCtt                                                       <  1:225231/148‑1 (MQ=255)
                                                                                        cggcgCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTg                                                      <  4:150897/149‑1 (MQ=255)
                                                                                         ggcgCTGGGCGGGGTGCTGGGCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGt                                                     <  4:315178/149‑1 (MQ=255)
                                                                                          gcgcTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGTGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTc                                                    <  7:335283/149‑1 (MQ=255)
                                                                                          gcgcTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTc                                                    <  7:256188/149‑1 (MQ=255)
                                                                                                gTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTa                                               <  5:338292/148‑1 (MQ=255)
                                                                                                       tgCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGaa                                        >  7:163319/1‑148 (MQ=255)
                                                                                                        gCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGaaa                                       >  7:113556/1‑148 (MQ=255)
                                                                                                            gTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAAccc                                  <  8:113556/149‑1 (MQ=255)
                                                                                                            gTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAAccc                                  >  7:192259/1‑149 (MQ=255)
                                                                                                            gTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTACAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAAccc                                  >  8:262450/1‑149 (MQ=255)
                                                                                                               aCCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGtt                               <  8:270845/149‑1 (MQ=255)
                                                                                                                ccAAACTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGCTCTGAGCGTTCAGCAGCTGGTATTGTCCTTTATCCCGAAAAACCCGttt                              >  5:84547/1‑149 (MQ=255)
                                                                                                                 cAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTc                                                                    <  8:131089/110‑1 (MQ=255)
                                                                                                                 cAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTc                                                                    <  8:126182/110‑1 (MQ=255)
                                                                                                                 cAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTc                                                                    >  7:126182/1‑110 (MQ=255)
                                                                                                                 cAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTc                                                                    >  7:131089/1‑110 (MQ=255)
                                                                                                                 cAACCTGGTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTg                             >  2:143847/1‑149 (MQ=255)
                                                                                                                    ccTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGcc                           >  2:151523/1‑148 (MQ=255)
                                                                                                                     cTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTATGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGCGCGTTCCGCAGCTGGTATTGTCCTTTATCCCGAAAAACCCGTTTGCCGa                         >  1:258328/1‑149 (MQ=255)
                                                                                                                      tGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTa                                               <  3:281562/126‑1 (MQ=255)
                                                                                                                      tGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTa                                               >  4:281562/1‑126 (MQ=255)
                                                                                                                        tttggtttgACGGCTGAAGGTCTGGTTCAGGGTGTTGCAGAAACTTCACGTCTGAACGCCATTTAAAGTAACTATGTTGTTAAATTCTCTGATATGAGATGTACGCAGCTGGTCTTGTCCTTTATAACGAAATACAAGTTTTCCGATCt                      >  5:239780/1‑149 (MQ=255)
                                                                                                                        tttggtttgACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAACCCCGTTTGCCGATCt                      >  7:112667/1‑149 (MQ=255)
                                                                                                                        tttggtttgACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCTTTTGCCGAt                        <  1:341335/147‑1 (MQ=255)
                                                                                                                          tggtttgACGGCTGAAGGTCTGGCTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTACCCCGAAAAACCCGTTTGCCGATCTTa                    >  8:319575/1‑149 (MQ=255)
                                                                                                                                gACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGa               <  7:44224/148‑1 (MQ=255)
                                                                                                                                gACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGa               <  2:258302/148‑1 (MQ=255)
                                                                                                                                      tGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATc        >  4:280553/1‑149 (MQ=255)
                                                                                                                                        aaGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCAAATcc       >  1:273725/1‑148 (MQ=255)
                                                                                                                                            tCTGGTTCAGGGTGGTGCAGAACCTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCGACGt  >  7:310590/1‑149 (MQ=255)
                                                                                                                                             cTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCGGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCGACGt  >  7:302572/1‑148 (MQ=255)
                                                                                                                                                    |                                                                                                                                            
AGCGCGGTTGCCCGTCTGCATAACGCATCTCAGTTAGGCAAAATCAGTTTTCTGACCATCGGTACGCTTTTGTTTACCACGCTGATTGCGGCGCTGGTCGGTGTGCTGGTCACCAACCTGTTTGGTTTGACGGCTGAAGGTCTGGTTCAGGGTGGTGCAGAAACTGCACGTCTGAACGCCATTGAAAGTAACTATGTTGGTAAAGTCTCTGATCTGAGCGTTCCGCAGCTGGTCTTGTCCTTTATCCCGAAAAACCCGTTTGCCGATCTTACCGGAGCCAATCCGACGT  >  NC_000913/1811201‑1811489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑