Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,757,908 C→T 5.5% G273D (GGC→GAC)  ldtE ← murein L,D‑transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,757,9080CT 5.5% 235.2 / 2.4 72G273D (GGC→GAC) ldtEmurein L,D‑transpeptidase
Reads supporting (aligned to +/- strand):  ref base C (31/37);  new base T (2/2);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCACCGGCGTTCCCGTCC  >  NC_000913/1757762‑1758052
                                                                                                                                                  |                                                                                                                                                
cATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCct                                                                                                                                                >  7:26380/1‑149 (MQ=255)
   tCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCctgct                                                                                                                                             >  2:202223/1‑149 (MQ=255)
        gcTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAg                                                                                                                                        <  1:351849/149‑1 (MQ=255)
              tctACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTa                                                                                                                                  >  7:337241/1‑149 (MQ=255)
                tACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATg                                                                                                                                >  3:274267/1‑149 (MQ=255)
                  cTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTAt                                                                                                                                 <  4:250442/146‑1 (MQ=255)
                    gAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCctgctt                                                                                                                                            >  4:116660/1‑132 (MQ=255)
                    gAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCctgctt                                                                                                                                            <  3:116660/133‑2 (MQ=255)
                     aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCAtt                                                                                                                           <  1:189457/149‑1 (MQ=255)
                     aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCAtt                                                                                                                           <  4:141926/149‑1 (MQ=255)
                     aaGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCAt                                                                                                                            <  8:32668/148‑1 (MQ=255)
                         cgcATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGGCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTAGGGCATTGTCt                                                                                                                       <  5:256492/149‑1 (MQ=255)
                          gcATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCt                                                                                                                       >  6:289555/1‑148 (MQ=255)
                                ctggctgCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGtt                                                                                                                >  7:225192/1‑149 (MQ=255)
                                   gctgCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTctg                                                                                                             <  5:289555/149‑1 (MQ=255)
                                       cGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTctgctg                                                                                                          <  8:26380/148‑1 (MQ=255)
                                       cGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTctgct                                                                                                           <  8:337241/147‑1 (MQ=255)
                                        gggAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGtt                                                                                                        <  8:225192/149‑1 (MQ=255)
                                         ggAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGttc                                                                                                       >  7:14105/1‑149 (MQ=255)
                                         ggAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGttc                                                                                                       >  5:215166/1‑149 (MQ=255)
                                          gAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGTCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGttct                                                                                                      >  6:306382/1‑149 (MQ=255)
                                          gAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGTCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGttct                                                                                                      >  6:311595/1‑149 (MQ=255)
                                             ttGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTtcttct                                                                                                   <  7:321137/149‑1 (MQ=255)
                                                cTCCACTGCTCACCGAAACCGGAAACCCAGCCCGACGATACAACGCTTAATCGACTAACTTCACATCTACAGCCTTATAGTCAATAAAAAGAGTAAAGCCATCTGGAAGTGTCTAAGGCAATAGCTCAACCTACAGCTGTTGTTCAGcc                                                                                                >  8:139584/1‑149 (MQ=255)
                                                   cACTTCTCACCGAAACCGTATAGACTGCCCGACGATATAACTCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGTCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGAt                                                                                             <  3:63776/149‑1 (MQ=255)
                                                     cTGCTCACCGAAACCGGATACCCTGCCCGACTAAACAACGCTTTATCGACTAACTTCTGATCTACATCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAg                                                                                           <  3:251139/149‑1 (MQ=255)
                                                      tGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGt                                                                                          >  8:316760/1‑149 (MQ=255)
                                                       gCTCACCGACACCGCATACCCTGCCCGACGATACAACGCTTTATCGACTAACTACTGATCAACAGCCTTATTGTCTATAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAggg                                                                                         <  4:90715/149‑3 (MQ=255)
                                                        cTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTgg                                                                                        >  7:115938/1‑149 (MQ=255)
                                                           aCCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGCTAGTGGTCg                                                                                     >  2:329360/1‑149 (MQ=255)
                                                           aCCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGt                                                                                       <  4:274267/147‑1 (MQ=255)
                                                           aCCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTc                                                                                      >  8:142979/1‑148 (MQ=255)
                                                              gAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATg                                                                                  >  2:64577/1‑149 (MQ=255)
                                                                      aTACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCaa                                                                          <  5:335663/149‑1 (MQ=255)
                                                                      aTACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCa                                                                           >  5:274045/1‑148 (MQ=255)
                                                                          ccTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACAt                                                                       >  3:61088/1‑148 (MQ=255)
                                                                              cccGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAAc                                                                    <  7:142979/147‑1 (MQ=255)
                                                                              cccGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGc                                                                  <  6:318232/149‑1 (MQ=255)
                                                                                      tACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGt                                                           >  7:186731/1‑148 (MQ=255)
                                                                                      tACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGt                                                           >  2:13841/1‑148 (MQ=255)
                                                                                         aaCGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTa                                                         <  2:324746/147‑1 (MQ=255)
                                                                                         aaCGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTa                                                         >  1:324746/1‑147 (MQ=255)
                                                                                           cGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCt                                                     >  7:283376/1‑149 (MQ=255)
                                                                                              tttATCGGCTAACTTCTGATCTACGGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTTGGCTcct                                                  >  4:99741/1‑148 (MQ=255)
                                                                                              gttATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGGGTATGGCATTGTCTGAACGTGCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCa                                                  <  7:306963/148‑1 (MQ=255)
                                                                                                tATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCa                                                  <  6:215166/147‑1 (MQ=255)
                                                                                                       tAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATAtt                                         >  1:79631/1‑149 (MQ=255)
                                                                                                         aCTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATAttt                                        <  3:79520/148‑1 (MQ=255)
                                                                                                         aCTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATAttt                                        >  7:126598/1‑148 (MQ=255)
                                                                                                         aCTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATAtt                                         <  8:283376/147‑1 (MQ=255)
                                                                                                           ttCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCAc                                     <  8:126598/149‑1 (MQ=255)
                                                                                                                    aCAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGtt                            >  1:166679/1‑149 (MQ=255)
                                                                                                                     cAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCAc                                     <  1:202223/139‑1 (MQ=255)
                                                                                                                     cAGCCTTATTGTCTTTAAATTGCGTAAACCCTGCTGGCAGTGTGTAGGGCATTGTCTGAACGGTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGtt                            <  6:273349/148‑1 (MQ=255)
                                                                                                                     cAGCCTTATTGTCTTTAAATTGCCTAAAGCCTGCTGGCAGTGTGTATGGCATTGGCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGGTCGtt                            <  2:332283/148‑1 (MQ=255)
                                                                                                                       gCCTTATTGGCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACTTTCTGCGGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGAt                         <  3:93608/149‑1 (MQ=255)
                                                                                                                        ccTTATTGTCTTTAAATTGCGTAAAGCCGGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCCCCGGTTCGTTGATc                        <  4:305900/149‑1 (MQ=255)
                                                                                                                          ttATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGACATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCAc                      >  1:5054/1‑149 (MQ=255)
                                                                                                                            aTTGTCTTTAAATTGCGTAAAGTCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTTCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACt                     <  5:311595/148‑1 (MQ=255)
                                                                                                                            aTTGTCTTTAAATTGCGTAAAGTCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACt                     <  5:306382/148‑1 (MQ=255)
                                                                                                                            aTTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACt                     <  8:14105/148‑1 (MQ=255)
                                                                                                                             ttGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACttt                   >  7:198021/1‑149 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAATCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTGCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCAc                <  3:237927/149‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTa                                                         <  7:258608/108‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTa                                                         >  8:258608/1‑108 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAg                                                        >  7:55819/1‑109 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAg                                                        <  8:55819/109‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCAc                <  8:186731/149‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCAc                <  2:166679/149‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGACATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCa                 <  2:5054/148‑1 (MQ=255)
                                                                                                                                tCTTTAAATTGCGTAAAGCCTGCGGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCCACATAACGCTGCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCAc                >  4:158318/1‑149 (MQ=255)
                                                                                                                                              aaaGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCACCGGCGTTCCCGTcc  >  3:37587/1‑149 (MQ=255)
                                                                                                                                                  |                                                                                                                                                
CATTCTGCGCTGACTCTACTGAAGGCGCATTGCTGGCTGCGGGAGTTGCTCCACTGCTCACCGAAACCGGATACCCTGCCCGACGATACAACGCTTTATCGACTAACTTCTGATCTACAGCCTTATTGTCTTTAAATTGCGTAAAGCCTGCTGGCAGTGTGTATGGCATTGTCTGAACGTTCTGCTGTTCTTCTGCCGATAGTGGTCGATGTACTTCAACATAACGCATCCCGTTAGGCTCCACGGAATATTTCACCGGTTCGTTGATCACTTTCACCGGCGTTCCCGTCC  >  NC_000913/1757762‑1758052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑