| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_000913 | 1,708,854 | C→G | 25.8% | Q646E (CAA→GAA) | rsxC → | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,708,854 | 0 | C | G | 25.8% | 18.9 / 6.7 | 19 | Q646E (CAA→GAA) | rsxC | SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex |
| Reads supporting (aligned to +/- strand): ref base C (11/3); new base G (3/2); total (14/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.48e-01 | |||||||||||
GAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCG > NC_000913/1708755‑1708998 | gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc < 8:44347/137‑1 (MQ=255)gaagaaCAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGc > 7:44347/1‑137 (MQ=255) ccgccgTCGAAGCCGCTATTGCCCGAGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAAGAACAGGTGGATCCGCGCAAAGCCGCCGTAGCAGCGGCTATTGCCCGCGCGCAAGCCCG‑AAACGACGAACAGCag > 4:10523/1‑148 (MQ=11) gCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCGGCCGTTGCAGCCGCTATTGCCCGCGCACAGGCCCGCAAACG‑CGCCCAGCAGCAGGCTGAAGCGGAGcact > 5:70482/1‑145 (MQ=14) gTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCGAACG‑GGAACAGCACCAGGCTAGTGCGGAGCAggaaga > 8:12762/1‑148 (MQ=14) gCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATACGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAggg > 3:319665/1‑137 (MQ=255) cctccaaCAGCAACTGTATAATTCATAGGCAGATCAACAGTGCGATCCGCGCAAAGAAGCATTCGAAGCCGCTATTGCCGTAGCCAATGCGAGCAATCT‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGt < 4:319665/133‑1 (MQ=255) aaCAGGCTAATGCCGAGCCAGAACAACAGGTAGATCCGCGCAAAGCGGCCGTAGAAGCGGCTATTGCCCGCGCAAAAGCAACCAAACG‑GGCACAGCTACCGGCTAATGCTTAACCATAAGGAAAGTTTGCTCCGCGGACAGCTGCAGtg > 6:209738/1‑148 (MQ=255) aGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCAGTAGAAGCGGCTATTGCCCGCGCACAAGCCAGCAAACG‑CGACCAGCAGAAGGATAATAAGGATCGAGAAGACCAGGTGTATCGGCACAAAGCTgaagtta > 4:272198/1‑141 (MQ=2) gCTAATGCGGAACCAGAAGAACTGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCg < 4:14860/96‑1 (MQ=11) gCTAATGCGGAACCAGAAGAACTGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCg > 3:14860/1‑96 (MQ=11) tGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCACCGCTGACGTCGAGGCGGCTa > 3:146195/1‑149 (MQ=255) aaccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACT‑GGAACa > 2:48367/3‑79 (MQ=11) aaccAGAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACa < 1:48367/77‑1 (MQ=255) accAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGaa > 8:112134/2‑76 (MQ=255) ccAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCGGCTATTGCCCGCGCAAAAGAGCTCACAAG‑AGCACAGCATCCGGCTAATGAGGAAAGATTAGAGCAGGTGGATCCGCGCAAAGCGGCAGTAGAAGCGGCTATGGCCcg > 6:108134/1‑149 (MQ=2) gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTaa < 5:149497/86‑1 (MQ=255) gAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTaa > 6:149497/1‑86 (MQ=255) gAACAACAGGTCGATCCGCGCAAAGCCGCCGTAGCAGCGGCTATTGCCCGCGCACAGGCCAGCAAACC‑CGACCAGCACCGGGCTACTGCGTGTACAGAAGAACAGGTTGCTCATCTCAAAGAGGCCGTGGACGCGGCTTTAGCAcgcg > 2:291436/1‑148 (MQ=9) | GAAGAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGTGCCAAAGCACGCAAGCTGGAACAGCAACAGGCTAATGCCGAGCCAGAACAACAGGTCGATCCGCGCAAAGCCGCCGTCGAAGCCGCTATTGCCCGAGCCAAAGCGCGCAAACG‑GGAACAGCAACCGGCTAATGCGGAGCCAGAAGAACAGGTTGATCCGCGCAAAGCTGCCGTCGAAGCGGCTATTGCACGCG > NC_000913/1708755‑1708998 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |