Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 908,491 C→T 5.8% A268A (GCG→GCA)  ltaE ← L‑allo‑threonine aldolase, PLP‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913908,4910CT 5.8% 221.5 / 2.4 70A268A (GCG→GCA) ltaEL‑allo‑threonine aldolase, PLP‑dependent
Reads supporting (aligned to +/- strand):  ref base C (34/31);  new base T (2/2);  total (37/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.72e-01

GTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCATTTTCCGCCAGC  >  NC_000913/908353‑908639
                                                                                                                                          |                                                                                                                                                    
gTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCtt                                                                                                                                            <  6:125168/149‑1 (MQ=255)
gTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCtt                                                                                                                                            >  2:113053/1‑149 (MQ=255)
    aGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACg                                                                                                                                        >  4:262313/1‑149 (MQ=255)
    aGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACg                                                                                                                                        <  8:252092/149‑1 (MQ=255)
     gATGCGTCACCAGGCTGACAATCGGAGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGc                                                                                                                                       <  5:133/149‑1 (MQ=255)
      aTGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCa                                                                                                                                      <  3:190171/149‑1 (MQ=255)
         cGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAgc                                                                                                                                    <  3:82191/148‑1 (MQ=255)
             aCCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTc                                                                                                                               >  8:197308/1‑149 (MQ=255)
               cAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCg                                                                                                                             <  7:313405/149‑1 (MQ=255)
                     gACACTGGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTATCGCGGCAGCATTTTCTTGCCCTACGCTAACAAACAGCATATTCGTGTCCTGAAGCATCACATCCGCTCCTGCTTCACGCAGCTGCTCCGCCATcc                                                                                                                       <  8:273456/149‑1 (MQ=255)
                     gACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATcc                                                                                                                       <  2:32208/149‑1 (MQ=255)
                      aCAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATc                                                                                                                        >  1:190636/1‑147 (MQ=255)
                         aTCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCGGACGCGAACAAACAGCATATTGGTGTCCTGCCGCATCACATCACCGCCAGCTTCACGCAGCAGCTCCGCCATCCAgg                                                                                                                    >  4:76840/1‑148 (MQ=255)
                          tCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCa                                                                                                                          >  5:61504/1‑141 (MQ=255)
                          tCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCa                                                                                                                          <  6:61504/141‑1 (MQ=255)
                               gAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGtt                                                                                                             <  2:67674/149‑1 (MQ=255)
                               gAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGtt                                                                                                             >  4:153616/1‑149 (MQ=255)
                                  gCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTc                                                                                                          >  2:107341/1‑149 (MQ=255)
                                    gTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGCCGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGt                                                                                                        >  7:327770/1‑149 (MQ=255)
                                    gTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCTGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGt                                                                                                        >  5:174093/1‑149 (MQ=255)
                                    gTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCTGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGt                                                                                                        >  5:169102/1‑149 (MQ=255)
                                    gTTAATCAGCACGTTTCTCGCTTTCAAGTATTCGCCTAACGCCGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCAGACGCATCACATCCGCGCCTGCTTCACGCACCTGCTCCCCCAACCAGGCAGCGTTGTCGt                                                                                                        >  7:323152/1‑149 (MQ=255)
                                     ttAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTg                                                                                                       <  1:97271/149‑1 (MQ=255)
                                     ttAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTg                                                                                                       >  3:256594/1‑149 (MQ=255)
                                       aaTCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTgg                                                                                                      <  1:298147/148‑1 (MQ=255)
                                       aaTCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGt                                                                                                     >  1:180688/1‑149 (MQ=255)
                                           aGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTc                                                                                                 <  5:147969/149‑1 (MQ=255)
                                           aGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTc                                                                                                 <  1:113053/149‑1 (MQ=255)
                                             cACTTTTCTCGCTTTCAGGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAACTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCt                                                                                               <  4:321525/149‑1 (MQ=255)
                                             cACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCt                                                                                               <  3:262313/149‑1 (MQ=255)
                                               cGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTg                                                                                              >  4:196038/1‑148 (MQ=255)
                                                     tcGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAgcgc                                                                                       >  1:160501/1‑149 (MQ=255)
                                                            cATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGt                                                                                >  7:171881/1‑149 (MQ=255)
                                                             aTGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGt                                                                                <  2:160501/148‑1 (MQ=255)
                                                              tGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTa                                                                              >  3:333063/1‑149 (MQ=255)
                                                              tGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTa                                                                              >  1:350970/1‑149 (MQ=255)
                                                                tATGCGCCTAACGCGGCAGCATTTTCTTCCCCGCCGCGAACAACCAGCATCTTGGCGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCGCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTAt                                                                             <  5:176335/148‑1 (MQ=255)
                                                                 aTTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGAGTTGTCGTGGTATTCCTGCAAGCGCGCAACGTTAttt                                                                           >  4:190812/1‑149 (MQ=255)
                                                                     gCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTAttttt                                                                         >  3:173669/1‑147 (MQ=255)
                                                                     gCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCa                                                                       <  4:277021/149‑1 (MQ=255)
                                                                     gCCTAACGCGGCAGCATTTTCTT‑CCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCa                                                                       <  7:350371/148‑1 (MQ=255)
                                                                      ccTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAg                                                                      <  7:197308/149‑1 (MQ=255)
                                                                        tAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAggg                                                                    >  1:61665/1‑149 (MQ=255)
                                                                          aCGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCa                                                                  >  6:98465/1‑149 (MQ=255)
                                                                          aCGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGACAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCa                                                                  >  6:173328/1‑149 (MQ=255)
                                                                                    aTTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACAACCGCTCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTAGTTTTCAGGGCATATATCCcgg                                                        >  2:327741/1‑149 (MQ=255)
                                                                                     ttttCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAg                                                                                          >  3:276454/1‑114 (MQ=255)
                                                                                     ttttCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAg                                                                                          <  4:276454/114‑1 (MQ=255)
                                                                                        tcttcCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGGTGTCGTGGGCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCcggcgg                                                     <  7:304449/148‑1 (MQ=255)
                                                                                             cccGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGc                                                 >  1:77257/1‑147 (MQ=255)
                                                                                             cccGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGc                                                 >  8:97048/1‑147 (MQ=255)
                                                                                             cccGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCa                                               <  4:333063/149‑1 (MQ=255)
                                                                                              ccGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCatc                                                 >  4:8426/1‑144 (MQ=255)
                                                                                               cGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTCGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAg                                              >  2:292740/1‑148 (MQ=255)
                                                                                                gACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGaa                                            <  7:97048/149‑1 (MQ=255)
                                                                                                gACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCACATATCCCGGCGGCAGCCAGaa                                            <  4:256594/149‑1 (MQ=255)
                                                                                                 aCGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAAt                                           <  3:196038/149‑1 (MQ=255)
                                                                                                   gcgAACAAACAGCATATTGGTGTCCTGACGCATCACATCTGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGc                                         <  6:174093/149‑1 (MQ=255)
                                                                                                   gcgAACAAACAGCATATTGGTGTCCTGACGCATCACATCTGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGc                                         <  6:169102/149‑1 (MQ=255)
                                                                                                      aacaaacaGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCg                                       >  8:182110/1‑148 (MQ=255)
                                                                                                        caaacaGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGAc                                    <  1:107341/149‑1 (MQ=255)
                                                                                                            caGCATATTGGTGTCCTGACTCCTCACATCCGCGCCTGCTTCACGCAGCTGCGCCGCGATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCTCAACGTTATTTTTCCGGGCATATATCCCGGCGGCAGCCAGACTGCCGGACTGgc                                <  8:166817/149‑1 (MQ=255)
                                                                                                            caGCATATTGGTGTCCTGACGCATCAAATCCGCGCCTGCTTCACGCAGCTGCTCCGCCTTCCAGGAAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCACAATGCCGGACTGgc                                >  2:191843/1‑149 (MQ=255)
                                                                                                            caGCATATTCGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGGTATGTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGCACTGgc                                >  8:13669/1‑149 (MQ=255)
                                                                                                                 tatTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATc                           <  8:74342/149‑1 (MQ=255)
                                                                                                                              aCGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCa              >  5:225556/1‑149 (MQ=255)
                                                                                                                              aCGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGACAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCa              <  5:173328/149‑1 (MQ=255)
                                                                                                                                gCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCAtt            >  4:108148/1‑149 (MQ=255)
                                                                                                                                  aTCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCCAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCAtttt          >  5:19393/1‑149 (MQ=255)
                                                                                                                                  aTCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCAtttt          >  1:335578/1‑149 (MQ=255)
                                                                                                                                          cgcgCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCATTTTCCGCCAg   <  2:61665/148‑1 (MQ=255)
                                                                                                                                          cgcgCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCATTTTCCGCCAGc  <  5:98465/149‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                                    
GTCAAGATGCGTCACCAGGCGGACAATCGGCGAGGCGTTAATCAGCACGTTTCTCGCTTTCATGTATTCGCCTAACGCGGCAGCATTTTCTTCCCCGACGCGAACAAACAGCATATTGGTGTCCTGACGCATCACATCCGCGCCTGCTTCACGCAGCTGCTCCGCCATCCAGGCAGCGTTGTCGTGGTCTTCCTGCAAGCGCGCAACGTTATTTTTCAGGGCATATATCCCGGCGGCAGCCAGAATGCCGGACTGGCGCATCCCGCCACCTGTCATTTTCCGCCAGC  >  NC_000913/908353‑908639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑