Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,291,110 A→T 6.7% K766I (AAA→ATA) ‡ yraJ → putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,291,1100AT 6.7% 240.1 / 6.0 80K766I (AAA→ATA) ‡yraJputative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (35/38);  new base T (3/2);  total (39/41)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.43e-01

GGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAT  >  NC_000913/3290964‑3291249
                                                                                                                                                  |                                                                                                                                           
ggTAGATAGTAGGCAGCTGCCCTATACCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGGGTACTTTTTACAGTCAGCGATCGCAACGGTaaa                                                                                                                                            <  5:21748/148‑1 (MQ=255)
ggTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAAc                                                                                                                                           <  7:288278/149‑1 (MQ=255)
     aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                             <  8:212008/142‑1 (MQ=255)
     aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTaa                                                                                                                                             >  7:212008/1‑142 (MQ=255)
     aTACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTg                                                                                                                                      <  2:239197/149‑1 (MQ=255)
       actacGCAGCTGCCCGATAACGTCGATCTTCAACAAACAACACAGTTAGTGGTGCCCAACAGAGGTACAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGcc                                                                                                                                    >  3:339774/1‑149 (MQ=255)
         tacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCt                                                                                                                                  >  8:190457/1‑149 (MQ=255)
         tacGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCACAACGGTTTACCGTTGcgat                                                                                                                                  >  5:268669/1‑146 (MQ=255)
           cGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCtt                                                                                                                                 <  8:128259/148‑1 (MQ=255)
            gCAGCTGCCCGATAACGTCGATCTTGAACAACCAACACAGTTTGTTGTGCCCAAAAGAGGTGCAATGGTAGCGGCGCGTTTCAACGGCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                               <  5:54645/149‑1 (MQ=255)
            gCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTAAAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTg                                                                                                                               <  6:53549/149‑1 (MQ=255)
             cAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCACAACGGTTTACCGTTGcgatcgct                                                                                                                              <  6:268669/149‑8 (MQ=255)
                    ccGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCtt                                                                                                                       >  1:209255/1‑149 (MQ=255)
                        tAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                   <  5:240666/149‑1 (MQ=255)
                         aaCGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCGAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCa                                                                                                                   <  2:89018/148‑1 (MQ=255)
                            gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                               >  3:284362/1‑149 (MQ=255)
                            gTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCaa                                                                                                               <  4:329620/149‑1 (MQ=255)
                                 tCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                           >  5:14423/1‑148 (MQ=255)
                                     gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCTACGGTAAACGGTTGCCCTTTGGCGCTCTTGCCAGCAACgatgat                                                                                                        <  4:339774/147‑1 (MQ=255)
                                     gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgatga                                                                                                         <  8:92005/146‑1 (MQ=255)
                                     gAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGGTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACgat                                                                                                           <  2:302619/144‑1 (MQ=255)
                                         aaacaaCACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACTGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgggg                                                                                                  >  7:166077/1‑149 (MQ=255)
                                           acaacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                                                                    >  2:371567/1‑145 (MQ=255)
                                           acaacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACgg                                                                                                    <  1:371567/145‑1 (MQ=255)
                                           acaacaCAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGc                                                                                                 >  4:318466/1‑148 (MQ=255)
                                                acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACTGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                           <  7:190457/149‑1 (MQ=255)
                                                acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGcaaca                                                                                             >  4:132764/1‑147 (MQ=255)
                                                acaGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                           <  8:54369/149‑1 (MQ=255)
                                                         ggtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTAtcgtc                                                                                   >  3:115839/1‑148 (MQ=255)
                                                         ggtgCCCAACAGAGGGGCAATGGTAGCGGCGCGTTTCACGGCCAATATCGGTTATCGCGTACTTGGTCCAGGCAGCGCTCGCAACGGTAGCCCGTTGGGGTTTGGCGGTGTTGGGAGCACCGATGCTGCGGGGCAAGAAAGTAgcgtgg                                                                                  >  2:273288/1‑147 (MQ=255)
                                                          gtgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGa                                                                                 <  4:363733/149‑1 (MQ=255)
                                                           tgCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAt                                                                                <  7:72953/149‑1 (MQ=255)
                                                             cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCGCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                              <  1:314080/149‑1 (MQ=255)
                                                             cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                              <  3:132764/149‑1 (MQ=255)
                                                             cccAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGa                                                                              <  4:284362/149‑1 (MQ=255)
                                                               cAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGGAACGATGAGACGGGGCAACAAAGTATCGTCGaggcgg                                                                            >  8:118657/1‑144 (MQ=255)
                                                                aaCAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAggg                                                                           >  8:409239/1‑149 (MQ=255)
                                                                       gggCAATGGTAGCGGCGCGTTTCACCGCCATTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATa                                                                    >  1:377315/3‑149 (MQ=255)
                                                                           aaTGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACtat                                                                <  7:409239/149‑1 (MQ=255)
                                                                                     gcgcGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTACACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTgg                                                      <  2:42307/149‑1 (MQ=255)
                                                                                      cgcgTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTggg                                                     <  6:14423/149‑1 (MQ=255)
                                                                                         gTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCCGCAACGATGATACGGGGCAACAATGTATCGGCGATGAGGGCGGCATACTATATCTCTCGGGGata                                                  >  8:211333/1‑149 (MQ=255)
                                                                                               aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                                                               <  4:82677/114‑1 (MQ=255)
                                                                                               aCGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATg                                                                               >  3:82677/1‑114 (MQ=255)
                                                                                               aCGCCAATATCGGCCAGCGCGTACTTGTTACAGTCACCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGt                                            <  7:119730/149‑1 (MQ=255)
                                                                                                 gCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTa                                           >  2:158241/1‑148 (MQ=255)
                                                                                                   cAATATCGGTTATCGCGTACTTGTTACAGTCAGCGCTCGCAACGGTAAACCGTTGCCGTTTGGCGCTCTTGCCCGCAACGATGATACGGGGCAACAAAGTATCGGCGAGGGGGGCGGCATTGTATTTCTCTCTgggtga                                                  >  6:86904/1‑136 (MQ=255)
                                                                                                   cAATAACGGTTATCGCGTACTTGTTACAGGCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGata                                                  <  5:86904/139‑1 (MQ=255)
                                                                                                    aaTATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGAAACGATGAAACGGGGCAACAAAGTATCGTCGATGAGGGCGGAATACTATATCTCTCTGGGATATCGAGTAAAt                                        >  1:257143/1‑148 (MQ=255)
                                                                                                      tatCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCACCGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCAAACTATATCTATCTGGGATATCTAGTAAATca                                      >  5:5860/1‑148 (MQ=255)
                                                                                                            ttATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTGTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAggt                               >  2:99788/1‑147 (MQ=255)
                                                                                                             tATCGCGTACTTGATACAGTCCCCGATCGCAACGCTAAACCGTTGCCCTTTCGCGCTCTTGCCAGCAACGATGACACCGGGCAACAATGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTg                              <  1:356087/149‑1 (MQ=255)
                                                                                                              aTCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTg                              >  1:422796/1‑148 (MQ=255)
                                                                                                               tCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGa                            <  4:115839/149‑1 (MQ=255)
                                                                                                                cgcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                           >  2:121955/1‑85 (MQ=255)
                                                                                                                cgcgTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACaaa                                                                                           <  1:121955/85‑1 (MQ=255)
                                                                                                                 gcgTACTTGGTACCGTCAGCGATCGCAACGGTAACCCGGTTCCCTTGGCCGCTCTAGCCAGCAACGAGGCTACGTGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACt                          <  6:213769/149‑1 (MQ=255)
                                                                                                                   gTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTg                         >  2:139925/1‑148 (MQ=255)
                                                                                                                    tACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa                       <  1:139925/149‑1 (MQ=255)
                                                                                                                    tACTTGTTACAGCCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAATTATCGTCGATGATGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTa                       <  7:118657/149‑1 (MQ=255)
                                                                                                                       ttGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGc                    >  7:204481/1‑149 (MQ=255)
                                                                                                                        tGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCt                   >  7:391550/1‑149 (MQ=255)
                                                                                                                         gTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTg                  >  5:87924/1‑149 (MQ=255)
                                                                                                                          ttACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTgg                             <  2:115439/137‑1 (MQ=255)
                                                                                                                          ttACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTgg                             >  1:115439/1‑137 (MQ=255)
                                                                                                                           tACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTggg                >  3:174525/1‑149 (MQ=255)
                                                                                                                             cAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGAGGAGGGCGGCATACTATATCGCTCTGGGATATCGAGTAAAGAACAAAGCTGGACTGTACGCTgggg               >  3:54568/1‑148 (MQ=255)
                                                                                                                              aGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                 >  7:196992/1‑97 (MQ=255)
                                                                                                                              aGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACta                                                                 <  8:196992/97‑1 (MQ=255)
                                                                                                                                tCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGTTGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCAGACGATATCTCTCTGGGAGATCGAGTAAATCACAAAGCTGGCCTGTCCGCTGGGGAAAt           >  6:303873/1‑149 (MQ=255)
                                                                                                                                   gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCt                   >  7:180539/1‑138 (MQ=255)
                                                                                                                                   gCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCt                   <  8:180539/138‑1 (MQ=255)
                                                                                                                                    cgctCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg       >  1:28288/4‑149 (MQ=255)
                                                                                                                                    cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAg                                 <  3:206168/123‑1 (MQ=255)
                                                                                                                                    cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAg                                 >  4:206168/1‑123 (MQ=255)
                                                                                                                                    cGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg       <  3:318466/149‑1 (MQ=255)
                                                                                                                                    aaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGt                                            >  5:112735/6‑112 (MQ=255)
                                                                                                                                    aaagggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGt                                            <  6:112735/107‑1 (MQ=255)
                                                                                                                                     gATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGACATACTATATCTCTCTGGGATATc                                                >  7:49932/1‑107 (MQ=255)
                                                                                                                                     gATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGACATACTATATCTCTCTGGGATATc                                                <  8:49932/107‑1 (MQ=255)
                                                                                                                                     gATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGAAACGGGCCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGc      <  3:14571/149‑1 (MQ=255)
                                                                                                                                        ggCAACGGTTTACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAgg       >  1:68374/2‑145 (MQ=255)
                                                                                                                                         gCAACGGTAAACCGTTGCCCATGGCCGCTCATGCCAGCAACGAGGCTACGGGGCAACACAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAt  <  3:248022/149‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                           
GGTAGATACTACGCAGCTGCCCGATAACGTCGATCTTGAACAAACAACACAGTTTGTGGTGCCCAACAGAGGTGCAATGGTAGCGGCGCGTTTCAACGCCAATATCGGTTATCGCGTACTTGTTACAGTCAGCGATCGCAACGGTAAACCGTTGCCCTTTGGCGCTCTTGCCAGCAACGATGATACGGGGCAACAAAGTATCGTCGATGAGGGCGGCATACTATATCTCTCTGGGATATCGAGTAAATCACAAAGCTGGACTGTACGCTGGGGAAATCAGGCAGAT  >  NC_000913/3290964‑3291249

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: