Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,034,691 G→A 5.0% pseudogene (641/663 nt) yedS → pseudogene, outer membrane protein homology; putative outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,034,6910GA 5.0% 254.8 / 2.3 79pseudogene (641/663 nt)yedSpseudogene, outer membrane protein homology; putative outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (42/33);  new base A (2/2);  total (44/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATG  >  NC_000913/2034544‑2034839
                                                                                                                                                   |                                                                                                                                                    
gCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGt                                                                                                                                                     >  4:37676/1‑149 (MQ=255)
  ttGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATTGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGCCTTCGGtt                                                                                                                                                    >  5:10687/1‑148 (MQ=255)
    gAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGttt                                                                                                                                                   <  4:111517/147‑1 (MQ=255)
      aCTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGt                                                                                                                                               >  7:262706/1‑149 (MQ=255)
        tttgctttgcAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCt                                                                                                                                             >  8:270130/1‑149 (MQ=255)
             tttgcAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGtg                                                                                                                                        >  7:319792/1‑149 (MQ=255)
                    tatCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTaa                                                                                                                                                 <  4:759/133‑1 (MQ=255)
                    tatCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTaa                                                                                                                                                 >  3:759/1‑133 (MQ=255)
                     atCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGAGTTCGACTTCGGTTTAAGTCTGGGTGCTGCatat                                                                                                                                >  7:315207/1‑149 (MQ=255)
                     atCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGtgc                                                                                                                                       >  5:390188/1‑142 (MQ=255)
                     atCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGtgc                                                                                                                                       <  6:390188/142‑1 (MQ=255)
                         gggTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGATTTAAGTCTGGGtgctgc                                                                                                                                    <  5:380607/141‑1 (MQ=255)
                         gggTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGATTTAAGTCTGGGtgctgc                                                                                                                                    >  6:380607/1‑141 (MQ=255)
                          ggTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCt                                                                                                                                             >  1:399501/1‑131 (MQ=255)
                          ggTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCt                                                                                                                                             <  2:399501/131‑1 (MQ=255)
                               taatGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGa                                                                                                                      >  5:34784/1‑149 (MQ=255)
                                aatGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGAt                                                                                                                     >  1:378571/1‑149 (MQ=255)
                                 atGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGAt                                                                                                                     <  5:178228/148‑1 (MQ=255)
                                 atGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGGTTAAGTCTGGGTGCTGCATATCAAACTCTGATc                                                                                                                    <  7:380404/149‑1 (MQ=255)
                                   gAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGt                                                                                                                  >  6:266289/1‑149 (MQ=255)
                                      aGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCa                                                                                                               <  2:378571/149‑1 (MQ=255)
                                          gtggACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATc                                                                                                           <  5:266289/149‑1 (MQ=255)
                                             gACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGt                                                                                                         >  8:214209/1‑148 (MQ=255)
                                             gACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGt                                                                                                         >  4:94932/1‑148 (MQ=255)
                                                cATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAgg                                                                                                     >  5:57612/1‑149 (MQ=255)
                                                  ttCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGATTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGtt                                                                                                   >  6:92266/1‑149 (MQ=255)
                                                        cAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGtt                                                                                             >  7:144053/1‑149 (MQ=255)
                                                         aGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTTAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTg                                                                                            <  5:130527/149‑1 (MQ=255)
                                                          ggAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTgg                                                                                           >  6:145378/1‑149 (MQ=255)
                                                           gAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGt                                                                                          <  3:37676/149‑1 (MQ=255)
                                                           gAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATAGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGt                                                                                          <  3:346501/149‑1 (MQ=255)
                                                              ggTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGttt                                                                                        >  6:39375/1‑148 (MQ=255)
                                                                   cGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGa                                                                                  <  6:34784/149‑1 (MQ=255)
                                                                   cGGTAGTGGTGATGGCCCTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGCTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCAAATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGa                                                                                  >  4:202032/1‑149 (MQ=255)
                                                                     gTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAACTGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATc                                                                                <  3:116311/149‑1 (MQ=255)
                                                                     gTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATc                                                                                <  8:144053/149‑1 (MQ=255)
                                                                          ggtgATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAAc                                                                           >  8:230515/1‑149 (MQ=255)
                                                                                    gTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCaa                                                                   >  7:343852/1‑147 (MQ=255)
                                                                                    gTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCaa                                                                   >  7:153501/1‑147 (MQ=255)
                                                                                     tAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCaa                                                                   >  8:83914/1‑146 (MQ=255)
                                                                                      aGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCa                                                                                                               <  8:314948/101‑1 (MQ=255)
                                                                                      aGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCa                                                                                                               >  7:314948/1‑101 (MQ=255)
                                                                                         cTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAAt                                                            >  5:34985/1‑149 (MQ=255)
                                                                                          tttCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATg                                                           >  3:145865/1‑149 (MQ=255)
                                                                                            tCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATg                                                           <  7:214209/147‑1 (MQ=255)
                                                                                            tCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGc                                                          <  3:94932/148‑1 (MQ=255)
                                                                                             ccAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCt                                                         <  8:262706/148‑1 (MQ=255)
                                                                                              cAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCtgg                                                       >  6:272130/1‑149 (MQ=255)
                                                                                               aaaGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCtggt                                                      >  2:4369/1‑149 (MQ=255)
                                                                                                 aGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCTGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCtggtgg                                                    <  7:327772/149‑1 (MQ=255)
                                                                                                  gAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTggtggt                                                   >  7:272313/1‑149 (MQ=255)
                                                                                                    aaaTGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGa                                                 <  6:34985/149‑1 (MQ=255)
                                                                                                     aaTTGTGATGGTGTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCGTGTTGCTGCATATCATACTCTGATCGTTCAGATCGTAAGTTTGGAGTTGGGTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGa                                                 <  2:357583/148‑1 (MQ=255)
                                                                                                      aTGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGaaa                                               <  5:145378/149‑1 (MQ=255)
                                                                                                        ggTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAAcc                                             >  7:247257/1‑149 (MQ=255)
                                                                                                        ggTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAAcc                                             >  5:122674/1‑149 (MQ=255)
                                                                                                            aTGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTg                                                                                                  >  7:158314/1‑92 (MQ=255)
                                                                                                            aTGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTg                                                                                                  <  8:158314/92‑1 (MQ=255)
                                                                                                               gTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGaa                                       <  8:153501/148‑1 (MQ=255)
                                                                                                               gTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGaa                                       <  7:75784/148‑1 (MQ=255)
                                                                                                                 ttCGGTATGTAAACCTCCTACGACTTCGACTTCGGTTTAAGTCGGGGTGCTGCATATCAAACTCTGATCGTTCATATCGTCAGGTAGGAGTTGGTTTAAATGATCGTAACCATAGCAAGAGAAATGCTGGTGGTGAAACCGATGAAGCa                                    >  6:158965/1‑149 (MQ=255)
                                                                                                                    ggTATGTCAACATCCTACGACTTCGACTTCGATTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATg                                  <  5:92266/148‑1 (MQ=255)
                                                                                                                     gTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGa                                >  4:360589/1‑149 (MQ=255)
                                                                                                                       aTGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACt                              >  3:394005/1‑149 (MQ=255)
                                                                                                                            aaCATCCTCCGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGGCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTgg                         >  6:115557/1‑149 (MQ=255)
                                                                                                                            aaCATACTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTgg                         >  3:81914/1‑149 (MQ=255)
                                                                                                                             aCATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGt                        <  7:83914/149‑1 (MQ=255)
                                                                                                                             aCATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGt                        <  7:270130/149‑1 (MQ=255)
                                                                                                                                tCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGc                      >  6:117953/1‑148 (MQ=255)
                                                                                                                                 ccTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCa                     >  4:99416/1‑148 (MQ=255)
                                                                                                                                   tACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCaaaa                  >  4:373064/1‑149 (MQ=255)
                                                                                                                                     cgacttcgacttCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAata                <  8:272313/149‑1 (MQ=255)
                                                                                                                                        cttcgacttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATatga             <  4:394005/149‑1 (MQ=255)
                                                                                                                                        cttcgacttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATatga             <  3:99416/149‑1 (MQ=255)
                                                                                                                                        cttcgacttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATatga             <  8:319792/149‑1 (MQ=255)
                                                                                                                                        cttcgacttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATatga             <  8:343852/149‑1 (MQ=255)
                                                                                                                                        cttcgacttcgGTTTAAGTCTGGCTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGGTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCGGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATatga             <  6:10687/149‑1 (MQ=255)
                                                                                                                                            gacttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGc          >  5:345808/1‑148 (MQ=255)
                                                                                                                                               ttcgGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCtaa       >  8:180772/1‑148 (MQ=255)
                                                                                                                                                  ggTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCtaataa    <  7:152397/148‑1 (MQ=255)
                                                                                                                                                   gTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAAtg  >  2:361915/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GCTTGAACTTTGCTTTGCAATATCAGGGTAATAATGAAAGTGGTGGACCATTCGGGCAGGAAGGTTCCGGTAGTGGTGATGGCCGTAGCCTTTCCAAAGAAAATGGTGATGGTTTCGGTATGTCAACATCCTACGACTTCGACTTCGGTTTAAGTCTGGGTGCTGCATATCAAACTCTGATCGTTCAGATCGTCAGGTTGGAGTTGGTTTAAATGATCGTAACCATAGCAACAGAAATGCTGGTGGTGAAACCGCTGAAGCATGGACTGTTGGTGCAAAATATGATGCTAATAATG  >  NC_000913/2034544‑2034839

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑