Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,311,510 C→T 5.7% A141V (GCA→GTA)  tonB → membrane spanning protein in TonB‑ExbB‑ExbD transport complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,311,5100CT 5.7% 223.5 / 2.1 70A141V (GCA→GTA) tonBmembrane spanning protein in TonB‑ExbB‑ExbD transport complex
Reads supporting (aligned to +/- strand):  ref base C (31/35);  new base T (2/2);  total (33/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAC  >  NC_000913/1311362‑1311658
                                                                                                                                                    |                                                                                                                                                    
ccgaagccgaaACCTAAGCCACAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGc                                                                                                                                                      <  5:404814/149‑1 (MQ=255)
  gaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCCCCGTTTGAAAATAAGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGTaa                                                                                                                                                    >  1:81088/1‑149 (MQ=255)
  gaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGTaa                                                                                                                                                    >  1:86683/1‑149 (MQ=255)
  gaagccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCaa                                                                                                                                                    <  4:145184/149‑1 (MQ=255)
     gccgaaACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCa                                                                                                                                                 <  3:268215/149‑1 (MQ=255)
          aaCCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAg                                                                                                                                            <  6:289804/149‑1 (MQ=255)
             cTAAGCCAAAACCGCAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCCCCGGTTGCAAATACCGCACCGGCACCCCAGCCATCAAGTACAGCAACGGCTGCAACCAGCACGCCg                                                                                                                                         >  7:336876/1‑149 (MQ=255)
             cTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGTCTGCAACCAGCAAGCCg                                                                                                                                         <  4:16142/149‑1 (MQ=255)
                   cAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGGCCGGCATCACCGTTTGAAAATACGGCACCGGCCCGCCGGACATCAAGTACAGCAACCGCTGCAACCCGCAACCCGGTGAc                                                                                                                                    >  5:315647/1‑148 (MQ=255)
                    aaaaCCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCCCGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTAc                                                                                                                                    >  7:312022/1‑147 (MQ=255)
                    aaaaCCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTAc                                                                                                                                    >  7:164552/1‑147 (MQ=255)
                           aaGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGtt                                                                                                                                      >  8:385109/1‑138 (MQ=255)
                           aaGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGtt                                                                                                                                      <  7:385109/138‑1 (MQ=255)
                              ccGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTc                                                                                                                        <  3:275980/149‑1 (MQ=255)
                                ggTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAg                                                                                                                      <  1:398975/149‑1 (MQ=255)
                                ggTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAg                                                                                                                      <  7:432897/149‑1 (MQ=255)
                                      aaaGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGTCGGTTACCAGTGTGGCTTCAgg                                                                                                                     >  4:153377/1‑144 (MQ=255)
                                      aaaGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGTCGGTTACCAGTGTGGCTTCAgg                                                                                                                     <  3:153377/144‑1 (MQ=255)
                                        aGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCAcgc                                                                                                              <  7:428400/149‑1 (MQ=255)
                                           tACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCa                                                                                                           >  3:362968/1‑149 (MQ=255)
                                             cAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCAt                                                                                                          >  5:141601/1‑148 (MQ=255)
                                                       cAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCAcgc                                                                                                              <  8:64074/134‑1 (MQ=255)
                                                       cAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCAcgc                                                                                                              >  7:64074/1‑134 (MQ=255)
                                                         aaaCGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCa                                                                                             <  2:220364/149‑1 (MQ=255)
                                                            cgcgATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGcc                                                                                          <  8:312022/149‑1 (MQ=255)
                                                               gATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCg                                                                                         >  5:417377/1‑147 (MQ=255)
                                                               gATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCg                                                                                         >  1:391032/1‑147 (MQ=255)
                                                                   tCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTAt                                                                                   <  8:104037/149‑1 (MQ=255)
                                                                     aaaCCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGTAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATc                                                                                  <  2:81088/148‑1 (MQ=255)
                                                                     aaaCCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGTAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATc                                                                                  <  2:86683/148‑1 (MQ=255)
                                                                     aaaCCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAAc                                                                                                                                                   >  6:112420/1‑83 (MQ=255)
                                                                     aaaCCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAAc                                                                                                                                                   <  5:112420/83‑1 (MQ=255)
                                                                       aCCCGTAGAGTCGCGTGCCGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATAAAGTACCGCCACGGCTGCCACCAGCAAGCCGGTTACCAGTGTGGCTTAAGGACCACGCGCATAAAGCCGTAATCAGCCCCAGTATCCgg                                                                               >  2:287495/1‑149 (MQ=255)
                                                                            tAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGa                                                                          <  6:417377/149‑1 (MQ=255)
                                                                                gTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAg                                                                                                                      <  2:324945/101‑1 (MQ=255)
                                                                                gTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAg                                                                                                                      >  1:324945/1‑101 (MQ=255)
                                                                                  cgcgTCCGGCAGCACCGTGTTAAAATACTGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCTACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAg                                                                    <  6:315647/149‑1 (MQ=255)
                                                                                   gcgTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCa                                                                                                                       <  5:608/97‑1 (MQ=255)
                                                                                   gcgTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCa                                                                                                                       >  6:608/1‑97 (MQ=255)
                                                                                      tCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAt                                                                >  7:3598/1‑149 (MQ=255)
                                                                                        cGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCAtt                                                               <  7:75182/148‑1 (MQ=255)
                                                                                             tCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCAt                                                         >  7:174268/1‑149 (MQ=255)
                                                                                             tCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCAt                                                         >  7:128468/1‑149 (MQ=255)
                                                                                               aCCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGcac                                                                      >  4:123039/1‑134 (MQ=255)
                                                                                               aCCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGcac                                                                      <  3:123039/134‑1 (MQ=255)
                                                                                               aCCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCAtt                                                        >  5:372832/1‑148 (MQ=255)
                                                                                                  gTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAg                                                    >  2:105628/1‑149 (MQ=255)
                                                                                                   tttGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAgg                                                   <  8:164552/149‑1 (MQ=255)
                                                                                                      gAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGTAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAggg                                                  >  8:165194/1‑147 (MQ=255)
                                                                                                       aaaaTACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATc                                                                                  >  8:299478/1‑114 (MQ=255)
                                                                                                       aaaaTACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATc                                                                                  <  7:299478/114‑1 (MQ=255)
                                                                                                       aaaaTACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAg                                               <  5:235759/149‑1 (MQ=255)
                                                                                                         aaTACGGCCCCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTCCCAGTGTGGCTTCAGGACCACGCGCATTAAGACGTAATCAGCCGCAGTATCAGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGgt                                             >  7:394572/1‑149 (MQ=255)
                                                                                                         aaTACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCa                                                <  1:300802/146‑1 (MQ=255)
                                                                                                         aaTACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCa                                                >  2:300802/1‑146 (MQ=255)
                                                                                                             cggcaccggcacGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGgttaaa                                         <  1:105628/149‑1 (MQ=255)
                                                                                                             cggcaccggcacGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGgttaaa                                         <  1:406359/149‑1 (MQ=255)
                                                                                                              ggcaccggcacGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAAt                                                                                               >  1:70483/1‑94 (MQ=255)
                                                                                                              ggcaccggcacGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAAt                                                                                               <  2:70483/94‑1 (MQ=255)
                                                                                                                    gtcaagCCTTACATAAAGTACAGCAACGGCTGCAACCAGATAGCCGGTTACGAGTGTGGCTTCAGGACCACGCGCATTATGCCGTAATCAGCCGCATTATCCGGCAAGAGCACAGGCTTTGAGCATTGAAGGGCAGGTTAAAGTTAAAt                                  <  8:394572/144‑1 (MQ=255)
                                                                                                                     gcacGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAAtt                                 >  2:324392/1‑149 (MQ=255)
                                                                                                                         gCCTGACATCAAGTACAGCAACGGCTGCAACCAGTAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGAc                             <  7:165194/149‑1 (MQ=255)
                                                                                                                         gCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGAc                             <  6:141601/149‑1 (MQ=255)
                                                                                                                         gCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGAc                             <  2:391032/149‑1 (MQ=255)
                                                                                                                          ccTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACg                            <  8:3598/149‑1 (MQ=255)
                                                                                                                           cTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGt                           >  7:399027/1‑149 (MQ=255)
                                                                                                                           cTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCACTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCAGTGAAGGGCAGGTTAAAGTTAAATTTGACGt                           >  1:75392/1‑149 (MQ=255)
                                                                                                                                aTCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGc                      >  1:1598/1‑149 (MQ=255)
                                                                                                                                      tACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATg                <  8:174268/149‑1 (MQ=255)
                                                                                                                                       aCAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGGAATCAGCCGCAGTATCCGGCACGCGCCCAGGCATTGCGCAGTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATgg               >  1:126391/1‑149 (MQ=255)
                                                                                                                                             aCGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGt         <  8:128468/149‑1 (MQ=255)
                                                                                                                                                    cAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAc  <  3:138412/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
CCGAAGCCGAAACCTAAGCCAAAACCGAAGCCGGTGAAAAAGGTACAGGAGCAGCCAAAACGCGATGTCAAACCCGTAGAGTCGCGTCCGGCATCACCGTTTGAAAATACGGCACCGGCACGCCTGACATCAAGTACAGCAACGGCTGCAACCAGCAAGCCGGTTACCAGTGTGGCTTCAGGACCACGCGCATTAAGCCGTAATCAGCCGCAGTATCCGGCACGAGCACAGGCATTGCGCATTGAAGGGCAGGTTAAAGTTAAATTTGACGTTACGCCGGATGGTCGCGTGGATAAC  >  NC_000913/1311362‑1311658

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑