| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,693,326 | C→G | R637R (CGG→CGC) | bcsA ← | cellulose synthase, catalytic subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,693,326 | 0 | C | G | 93.3% | 46.2 / ‑5.6 | 15 | R637R (CGG→CGC) | bcsA | cellulose synthase, catalytic subunit |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base G (6/8); minor base T (1/0); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.67e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACACCATACTGACGACCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGCCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCTTTGTGCGGGTTAATCAGCGCCACCAGCGTCGGTGGTGCGATATACCACGCCAGCACCGTTTCGTAGATTTCA > NC_000913/3693217‑3693467 | acacCATACTGACGACCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGt > 3:33661/1‑149 (MQ=255) cTGACGACCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCtt < 2:4258/148‑1 (MQ=255) acgacCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTg < 4:33661/147‑1 (MQ=255) acgacCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGc > 2:158388/1‑149 (MQ=255) acCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGt < 1:158388/149‑1 (MQ=255) acCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGt < 2:769/149‑1 (MQ=255) gCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTcc < 7:20281/111‑1 (MQ=255) gCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTcc > 8:20281/1‑111 (MQ=255) tACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCttt < 3:42710/117‑1 (MQ=255) tACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCttt > 4:42710/1‑117 (MQ=255) ccaccaGGTTGAGCAGGACAAGGAAGATGTAGGGTCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCAACCTTTGGCGGTGACGTTAAATTTGCCTTTGTGCGGGTTAATCAGCGCCACCAGCGTCGGTGGTGCGATATAc > 5:32938/1‑149 (MQ=255) accaGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTg < 5:25864/99‑1 (MQ=255) accaGGTTGAGCAGGACAAGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTg > 6:25864/1‑99 (MQ=255) aaGGAAGATGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCTTTGTGCGGGTTAATCAGCGCCACCAGCGTCGGTGGTGCGATATACCACGCCAGCACCGTTTCg > 5:169497/1‑148 (MQ=255) tGTAGGGGCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCTTTGTGCGGGTTAATCAGCGCCACCAGCGTCGGTGGTGCGATATACCACGCCAGCACCGTTTCGTAGATTTCa < 6:169497/149‑1 (MQ=255) | ACACCATACTGACGACCACGGTGAGCATCTCGGTTGGCGGGCCATAGAAGTAGCGCCAGATGCCTACCGCAACGCCCACCAGGTTGAGCAGGACAAGGAAGATGTAGGGCCGCGAGATCACCCAGTCGACGTACTCTTCTTCCACCAGTCCACCTTTGGCGGTGACGTTAAATTTGCCTTTGTGCGGGTTAATCAGCGCCACCAGCGTCGGTGGTGCGATATACCACGCCAGCACCGTTTCGTAGATTTCA > NC_000913/3693217‑3693467 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |