Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,556,903 | A→G | 7.5% | L132P (CTC→CCC) | eutC ← | ethanolamine ammonia‑lyase, small subunit (light chain) |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,556,903 | 0 | A | G | 7.5% | 155.2 / 2.4 | 53 | L132P (CTC→CCC) | eutC | ethanolamine ammonia‑lyase, small subunit (light chain) |
Reads supporting (aligned to +/- strand): ref base A (26/23); new base G (2/2); total (28/25) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
TCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGC > NC_000913/2556759‑2557051 | tctTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGAtt > 5:136153/1‑149 (MQ=255) ttCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGAtttt > 8:15105/1‑149 (MQ=255) cGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGAttt < 7:320281/146‑1 (MQ=255) gTAGTTCACGGTGATCGCATCAGTTGACAGGCCAGCAGAAATAACGACATCTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTGCTGCACACAGGCGGCGGCCCATATCCGGGTGAGTCAGGTAGAGATTTTTGt < 5:121581/149‑1 (MQ=255) tAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTc < 4:13892/149‑1 (MQ=255) aTCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCaga < 6:165083/149‑1 (MQ=255) tCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCagag > 1:175404/1‑149 (MQ=255) tCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTAc < 6:136153/149‑1 (MQ=255) tCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTAc < 2:291884/149‑1 (MQ=255) aGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACtt > 7:193890/1‑149 (MQ=255) cATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGccc > 5:257252/1‑149 (MQ=255) aTCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGGGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCt > 1:107028/1‑149 (MQ=255) tCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTg > 1:89998/1‑149 (MQ=255) tCAGAAATAACGACCTGTACGTCCGGATTCGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCCGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTg > 3:33959/1‑149 (MQ=255) aTACCGACCTGTACGTCCGGATTGGCAACACACTTCGCTTTCAGCTCTTCAACAGCATCTGCACACAGGCGGCGGCCACTATCCGGGCGAGTCCGGTAGAGATGTGTGTCGCGAATGTCAGAGCGTGCTTCCAGCCAGCCCTGCGCtgt > 2:117584/1‑147 (MQ=255) aCGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCAc < 5:61305/149‑1 (MQ=255) aCGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCAc < 7:15105/149‑1 (MQ=255) aCCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCa > 5:118680/1‑149 (MQ=255) cTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGt > 1:57572/1‑118 (MQ=255) cTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGt < 2:57572/118‑1 (MQ=255) tACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCActc < 4:46576/147‑1 (MQ=255) tACGTCCGGATTGGCAACACACTGCGCGTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCCAGCCCTGCGCTTTCACCCACTCtt > 6:149662/1‑149 (MQ=255) gTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGAGTTTTGTCGCTAAGTTCAGAGCGTACTTCCAGCCAGCCCTGCGCTTTCACCCACTCGTcct > 4:233937/1‑148 (MQ=255) ggATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCAc < 2:89998/149‑1 (MQ=255) aTTGGCAACACACTGCGCTTTCAGCGCTTCAACTGCTGCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCActt < 4:215511/149‑1 (MQ=255) gCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCttt < 8:42793/149‑1 (MQ=255) cAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCAGCGCTTTCACCCACTCTTCCGGCAtttctttc < 4:271662/149‑1 (MQ=255) cacTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTAc > 6:67588/1‑149 (MQ=255) acTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACg > 2:105537/1‑149 (MQ=255) cTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACgg > 5:308567/1‑149 (MQ=255) cgcTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTAt < 8:193890/149‑1 (MQ=255) tttCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCtt > 7:281894/1‑149 (MQ=255) aGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGGGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCttt > 6:222171/1‑131 (MQ=255) aGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGGGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCttt < 5:222171/131‑1 (MQ=255) gcgcTTCAACAGCTTCTGCACACCGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGaa < 1:371370/149‑1 (MQ=255) gcgcTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGGGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGaa < 3:324221/149‑1 (MQ=255) cTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGTCGAGTCCGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGAAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACga < 5:101238/149‑1 (MQ=255) cAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACgaga < 6:118680/148‑1 (MQ=255) cAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACgaga < 6:308567/148‑1 (MQ=255) cAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACgaga < 1:105537/148‑1 (MQ=255) aaCAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGCACGAGAGt > 5:312213/1‑149 (MQ=255) aaCAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGagag < 4:324940/148‑1 (MQ=255) cAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTGTCAGTACGGTATCTTTCGAACGAGAGTGa > 8:267307/1‑149 (MQ=255) gCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCGGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTAGGGTATCTTTCGAACGAGAGTGATCGGCCAg > 4:262981/1‑149 (MQ=255) cacacaGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAg > 8:121135/1‑148 (MQ=255) acacaGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAgg > 2:214396/1‑148 (MQ=255) acacaGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAgg > 5:329824/1‑148 (MQ=255) aGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACg > 4:168956/1‑149 (MQ=255) ggcggcCCATATCCGGGCGAGTCAGGTAGGGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACgcagc < 2:107028/149‑1 (MQ=255) tCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGAcgc < 3:168956/149‑1 (MQ=255) tgttgagaTTTTTTGCGCAAATTTCAGACCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCa < 2:233661/110‑1 (MQ=255) ggTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCa > 1:233661/1‑114 (MQ=255) ggTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGa > 6:214792/1‑149 (MQ=255) gTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGAc > 3:81444/1‑149 (MQ=255) gagaTTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCgg > 5:141153/1‑149 (MQ=255) agaTTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCgg < 6:257252/148‑1 (MQ=255) agaTTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCgg > 1:352563/1‑148 (MQ=255) agaTTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGc < 6:329824/149‑1 (MQ=255) | TCTTCGTAGTTCACGGTGATCGCATCAGTTGACAGGCCATCAGAAATAACGACCTGTACGTCCGGATTGGCAACACACTGCGCTTTCAGCGCTTCAACAGCTTCTGCACACAGGCGGCGGCCCATATCCGGGCGAGTCAGGTAGAGATTTTTGTCGCTAATTTCAGAGCGTACTTCCAGCAAGCCCTGCGCTTTCACCCACTCTTCCGGCACTTCTTTCAGTACGGTATCTTTCGAACGAGAGTGATCGGCCAGGAAACGCAGCAGCGCCTGCGTACGTGGACGCGGACCGGC > NC_000913/2556759‑2557051 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |