Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,017,871 G→A 5.5% A440T (GCG→ACG)  fliI → flagellum‑specific ATP synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,017,8710GA 5.5% 231.1 / 2.6 72A440T (GCG→ACG) fliIflagellum‑specific ATP synthase
Reads supporting (aligned to +/- strand):  ref base G (30/38);  new base A (2/2);  total (32/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.21e-01

TTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGC  >  NC_000913/2017724‑2018010
                                                                                                                                                   |                                                                                                                                           
ttCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAACGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAcgcgc                                                                                                                                            >  2:68587/1‑149 (MQ=255)
  cAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAcgcgcg                                                                                                                                           <  8:290410/148‑1 (MQ=255)
  cAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAAcgcgc                                                                                                                                            <  2:252686/147‑1 (MQ=255)
      cAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTg                                                                                                                                      <  7:10345/149‑1 (MQ=255)
             tgtCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCg                                                                                                                               >  6:307613/1‑149 (MQ=255)
              gtCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGt                                                                                                                              >  6:234025/1‑149 (MQ=255)
              gtCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGt                                                                                                                              >  6:141707/1‑149 (MQ=255)
               tCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGt                                                                                                                              >  4:50227/1‑148 (MQ=255)
               tCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGt                                                                                                                              <  5:141707/148‑1 (MQ=255)
                 gAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTggg                                                                                                                                    <  3:238250/140‑1 (MQ=255)
                 gAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTggg                                                                                                                                    >  4:238250/1‑140 (MQ=255)
                        cAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAgg                                                                                                                     <  5:226084/148‑1 (MQ=255)
                        cAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAgg                                                                                                                     <  8:14466/148‑1 (MQ=255)
                         aGCGTAACCGCGATCTGTTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGGGGCCGCAGCGGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAggg                                                                                                                    <  8:269099/148‑1 (MQ=255)
                         aGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGTCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTTCAgggg                                                                                                                   <  5:236353/149‑1 (MQ=255)
                          gCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGc                                                                                                                  >  1:36290/1‑149 (MQ=255)
                            gTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTg                                                                                                                <  3:50227/149‑1 (MQ=255)
                             tAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGATg                                                                                                                >  2:43806/1‑148 (MQ=255)
                             tAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTCTGTCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTg                                                                                                                >  8:273230/1‑148 (MQ=255)
                               agcgcgTTCTGGTTAGCGTCGGCGCGTATGCCATAGGCAGCGATCCGATGCTCTTTAATGCCTTCGCCCTTTGGCCTCAGCTGGAGGGCTATTTGCAACAAGTCATTTTTTAACGCGCGGACTGGTAAGCGTCTCTCCAggggattta                                                                                                              <  8:299414/146‑6 (MQ=255)
                               aCCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAgg                                                                                                                     <  2:320817/141‑1 (MQ=255)
                               aCCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAgg                                                                                                                     >  1:320817/1‑141 (MQ=255)
                                ccGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGc                                                                                                            <  3:251816/149‑1 (MQ=255)
                                ccGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGc                                                                                                            >  3:291699/1‑149 (MQ=255)
                                ccGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGc                                                                                                            <  2:348533/149‑1 (MQ=255)
                                ccGCGATCTGGTTAGCGTCGGCGCGTATACCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGc                                                                                                            >  4:71452/1‑149 (MQ=255)
                                 cgcgATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCg                                                                                                           >  3:88512/1‑149 (MQ=255)
                                    gATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTAt                                                                                                        <  2:36290/149‑1 (MQ=255)
                                                 tCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAgtg                                                                                           <  1:187391/149‑1 (MQ=255)
                                                    gcgcGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGa                                                                                                              >  4:120399/1‑127 (MQ=255)
                                                    gcgcGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGa                                                                                                              <  3:120399/127‑1 (MQ=255)
                                                        gTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAAc                                                                                    <  3:80948/149‑1 (MQ=255)
                                                          aTGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAATCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAAcc                                                                                   >  6:333599/1‑148 (MQ=255)
                                                             ccAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGCACGCGCGGCCTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCTTAACCCAg                                                                                >  8:320433/1‑148 (MQ=255)
                                                                   gCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCGGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATaa                                                                         <  1:95546/149‑1 (MQ=255)
                                                                     aGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAg                                                                                >  6:245913/1‑140 (MQ=255)
                                                                     aGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAg                                                                                <  5:245913/140‑1 (MQ=255)
                                                                            cGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGAt                                                                 <  4:291699/148‑1 (MQ=255)
                                                                                      tAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATg                                                      <  5:234025/149‑1 (MQ=255)
                                                                                            cATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCTAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTgcgc                                                <  1:130306/149‑1 (MQ=255)
                                                                                             aTCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCt                                               >  5:57446/1‑149 (MQ=255)
                                                                                                  ccTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCg                                                                                                 >  6:634/1‑94 (MQ=255)
                                                                                                  ccTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCg                                                                                                 <  5:634/94‑1 (MQ=255)
                                                                                                  ccTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACg                                                                       >  4:27459/1‑120 (MQ=255)
                                                                                                  ccTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACg                                                                       <  3:27459/120‑1 (MQ=255)
                                                                                                    tgtgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGAcc                                        >  8:215347/1‑149 (MQ=255)
                                                                                                      tgGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGAcc                                        <  4:88512/147‑1 (MQ=255)
                                                                                                        gCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTg                                     >  8:219652/1‑148 (MQ=255)
                                                                                                        gCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGa                                    <  1:43806/149‑1 (MQ=255)
                                                                                                           gCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGa                                    >  2:154307/1‑146 (MQ=255)
                                                                                                             aGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACgg                                                                      >  2:3534/1‑110 (MQ=255)
                                                                                                             aGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACgg                                                                      <  1:3534/110‑1 (MQ=255)
                                                                                                               cTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCt                             <  4:103339/149‑1 (MQ=255)
                                                                                                                tGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCt                                               >  4:178490/1‑130 (MQ=255)
                                                                                                                   aGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCa                         <  5:307613/149‑1 (MQ=255)
                                                                                                                     ggCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCt                             <  4:54259/143‑1 (MQ=255)
                                                                                                                     ggCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCt                             >  3:54259/1‑143 (MQ=255)
                                                                                                                          tttGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaaa                   <  7:215347/148‑1 (MQ=255)
                                                                                                                            tGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                 <  7:219652/148‑1 (MQ=255)
                                                                                                                            tGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCGGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAaga                 <  1:68587/148‑1 (MQ=255)
                                                                                                                               aacaaGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAgg               >  5:310192/1‑147 (MQ=255)
                                                                                                                               aacaaGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTa             <  5:333599/149‑1 (MQ=255)
                                                                                                                                    ggCATTTTTTAACGCGCGGACTGGGAAGCGTCTCTCCCGGGGCTGGTGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGa        <  7:273230/149‑1 (MQ=255)
                                                                                                                                    ggCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGa        <  6:57446/149‑1 (MQ=255)
                                                                                                                                       aTTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTagag          <  8:48969/144‑1 (MQ=255)
                                                                                                                                       aTTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTagag          >  7:48969/1‑144 (MQ=255)
                                                                                                                                       aTTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGc     >  8:307666/1‑149 (MQ=255)
                                                                                                                                        tttttGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCa                                                             >  6:25030/1‑92 (MQ=255)
                                                                                                                                        tttttGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCa                                                             <  5:25030/92‑1 (MQ=255)
                                                                                                                                        tttttGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAGGAGGTAGAGGATGcc    >  4:272423/1‑149 (MQ=255)
                                                                                                                                          tttGAACGCACGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTA‑TTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaa                    <  7:99942/130‑1 (MQ=255)
                                                                                                                                          tttGAACGCACGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTA‑TTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaa                    <  7:95048/130‑1 (MQ=255)
                                                                                                                                          tttGAACGCACGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTA‑TTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaa                    >  8:95048/1‑130 (MQ=255)
                                                                                                                                          tttGAACGCACGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTA‑TTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGaaaa                    >  8:99942/1‑130 (MQ=255)
                                                                                                                                           ttGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCcgc  <  3:71452/148‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
TTCAAACAGCTGTTGTCGAGTTTTCAGCGTAACCGCGATCTGGTTAGCGTCGGCGCGTATGCCAAAGGCAGCGATCCGATGCTCGATAAAGCCATCGCCCTGTGGCCGCAGCTGGAGGGCTATTTGCAACAAGGCATTTTTGAACGCGCGGACTGGGAAGCGTCTCTCCAGGGGCTGGAGCGTATTTTCCCGACAGTGTCATAACCCAGGAGATAACGGCAGATGGCAGAACATGGTGCGCTGGCGACCCTGAAAGATCTGGCAGAAAAAGAGGTAGAGGATGCCGC  >  NC_000913/2017724‑2018010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑