Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,935,902 G→T 5.2% A138D (GCT→GAT)  zwf ← glucose‑6‑phosphate 1‑dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,935,9020GT 5.2% 268.7 / 3.2 77A138D (GCT→GAT) zwfglucose‑6‑phosphate 1‑dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (38/35);  new base T (2/2);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.57e-01

AGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATC  >  NC_000913/1935755‑1936041
                                                                                                                                                   |                                                                                                                                           
aGGTGCAGCACCGTTTCTTTACCATGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCATCCTGATCATTGATTTCCTGCGAGGTCGCCAGCGAAGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGc                                                                                                                                            <  4:21259/149‑1 (MQ=255)
  gTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCAt                                                                                                                                          >  6:114374/1‑149 (MQ=255)
   ttCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCAtt                                                                                                                                         >  1:51395/1‑149 (MQ=255)
     cAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCAtt                                                                                                                                         >  3:277285/1‑147 (MQ=255)
         aCCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGttt                                                                                                                                   >  8:367432/1‑149 (MQ=255)
          ccGTTTCTTTACCAAGATAGTGGTCGTTACTGTAAACCTGGCACTCCTGGAAGTATTCGCCAAGCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGtttt                                                                                                                                  <  1:239875/149‑1 (MQ=255)
          ccGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGtttt                                                                                                                                  <  4:188387/149‑1 (MQ=255)
          ccGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGtttt                                                                                                                                  <  2:51395/149‑1 (MQ=255)
               tCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTc                                                                                                                             >  6:214356/1‑149 (MQ=255)
                     ccAAGATAGTGGGCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAg                                                                                                                       <  8:179386/149‑1 (MQ=255)
                      cAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGc                                                                                                                      >  1:121481/1‑149 (MQ=255)
                      cAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGc                                                                                                                      >  3:274199/1‑149 (MQ=255)
                           tAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCttt                                                                                                                 <  4:274199/149‑1 (MQ=255)
                                gTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCaaa                                                                                                            <  6:196512/149‑1 (MQ=255)
                                  cGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAAtt                                                                                                          >  6:331336/1‑149 (MQ=255)
                                  cGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAAtt                                                                                                          >  6:336782/1‑149 (MQ=255)
                                   gATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAAtt                                                                                                          <  2:48658/148‑1 (MQ=255)
                                   gATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTg                                                                                                         >  3:194031/1‑149 (MQ=255)
                                     tACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTgcg                                                                                                       <  4:194031/149‑1 (MQ=255)
                                          tAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCaa                                                                                                   >  8:296258/1‑148 (MQ=255)
                                             aCCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGt                                                                                               <  2:179578/149‑1 (MQ=255)
                                              ccTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTg                                                                                              <  4:277285/149‑1 (MQ=255)
                                                 ggCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTg                                                                                           >  4:268912/1‑149 (MQ=255)
                                                  gCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTgg                                                                                          <  5:331336/149‑1 (MQ=255)
                                                  gCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTgg                                                                                          <  5:336782/149‑1 (MQ=255)
                                                    aCTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCGCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTTCAAATTGCGCCAAAAGTGCTGggc                                                                                        <  4:46646/149‑1 (MQ=255)
                                                      tcctcGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGggcgg                                                                                      >  8:205036/1‑149 (MQ=255)
                                                      tcctcGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGggcgg                                                                                      >  7:35815/1‑149 (MQ=255)
                                                        ctcGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCaaaa                                                                                                 <  7:265021/136‑1 (MQ=255)
                                                        ctcGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCaaaa                                                                                                 >  8:265021/1‑136 (MQ=255)
                                                         tcGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCAt                                                                                   >  5:164294/1‑149 (MQ=255)
                                                           gAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATgg                                                                                 >  1:62473/1‑149 (MQ=255)
                                                           gAAGTAATAGCCAACCTGATCATTGATTTCCTGCGAGGTCGCAAGAGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGACGGTTTAGCATTCATTTTTGCATCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGCCATgc                                                                                 >  5:364697/1‑148 (MQ=255)
                                                            aaGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCttt                                                                                                                 >  6:74929/1‑116 (MQ=255)
                                                            aaGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCttt                                                                                                                 <  5:74929/116‑1 (MQ=255)
                                                             aGTATTCGCCACCCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGc                                                                                >  5:134837/1‑148 (MQ=255)
                                                               tATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCaaa                                                                             <  2:34518/149‑1 (MQ=255)
                                                                aTTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAag                                                                            >  1:243576/1‑149 (MQ=255)
                                                                   cGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCAGTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAagta                                                                          <  4:184897/148‑1 (MQ=255)
                                                                    gCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGGTGCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCGCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAagtagt                                                                        <  1:305114/149‑1 (MQ=255)
                                                                      cAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTa                                                                      <  7:296258/149‑1 (MQ=255)
                                                                        aCCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAAt                                                                    <  5:70040/149‑1 (MQ=255)
                                                                         ccTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATg                                                                   >  1:356350/1‑149 (MQ=255)
                                                                         ccTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATg                                                                   <  6:164294/149‑1 (MQ=255)
                                                                          cTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATgg                                                                  <  5:214356/149‑1 (MQ=255)
                                                                              tCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGa                                                               >  4:139129/1‑148 (MQ=255)
                                                                                aTTGATTTCCTGCGAGGTCGCCAGCGAAGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCTTTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATAc                                                            >  2:300433/1‑149 (MQ=255)
                                                                                    aTTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGACAAagtagt                                                                        <  7:41061/133‑1 (MQ=255)
                                                                                    aTTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGACAAagtagt                                                                        >  8:41061/1‑133 (MQ=255)
                                                                                      ttCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGAtttt                                                      >  8:84173/1‑149 (MQ=255)
                                                                                        ccTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGAttttt                                                     <  7:205036/148‑1 (MQ=255)
                                                                                         cTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGAttttttt                                                   >  5:130904/1‑149 (MQ=255)
                                                                                         cTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGAtttttt                                                    <  7:367432/148‑1 (MQ=255)
                                                                                                gTCGNCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTATCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAg                                            >  5:46785/1‑149 (MQ=255)
                                                                                                gTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTATCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAg                                            >  5:41814/1‑149 (MQ=255)
                                                                                                     cAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAagtagt                                                                        <  7:268276/116‑1 (MQ=255)
                                                                                                     cAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAagtagt                                                                        >  8:268276/1‑116 (MQ=255)
                                                                                                      aGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATcgc                                      >  5:278948/1‑149 (MQ=255)
                                                                                                               cccAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACg                             <  5:114374/149‑1 (MQ=255)
                                                                                                               cccAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCAGTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACg                             >  8:269179/1‑149 (MQ=255)
                                                                                                                  aGCGGTTTCTCCATGACTACGCGTGCCGGTTTATCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCt                          <  6:41814/149‑1 (MQ=255)
                                                                                                                  aGCGGTTTCTCCATGACTACGCGTGCCGGTTTATCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCt                          <  6:46785/149‑1 (MQ=255)
                                                                                                                     ggTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGaa                       <  6:278948/149‑1 (MQ=255)
                                                                                                                     ggTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGaa                       <  3:139129/149‑1 (MQ=255)
                                                                                                                     ggTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGaa                       <  3:268912/149‑1 (MQ=255)
                                                                                                                       tttCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATg                     >  1:160193/1‑149 (MQ=255)
                                                                                                                            ccATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcag                  >  6:320074/1‑147 (MQ=255)
                                                                                                                             cATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcag                  <  8:35815/146‑1 (MQ=255)
                                                                                                                             cATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGcagcag               >  5:125542/1‑149 (MQ=255)
                                                                                                                             cATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGcagcag               >  7:169478/1‑149 (MQ=255)
                                                                                                                              aTGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAgt              <  7:84173/149‑1 (MQ=255)
                                                                                                                                   tACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCAt         >  3:232235/1‑149 (MQ=255)
                                                                                                                                   tACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAAAGGTGATACGAGTTTTTTGAACCAGCATCGCGCCGAGCCGGCTGAATGCAGCAGTGTCAt         >  1:158467/1‑149 (MQ=255)
                                                                                                                                     cgcgTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCAtt        <  4:222239/148‑1 (MQ=255)
                                                                                                                                        gTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATgcag                  <  8:314114/135‑1 (MQ=255)
                                                                                                                                          gCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATc  >  8:65104/1‑149 (MQ=255)
                                                                                                                                             ggTTTAGCATTCAGTTTTGCCTCGCCACGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGAGTCATTGACATc  >  8:60523/1‑146 (MQ=255)
                                                                                                                                                   |                                                                                                                                           
AGGTTCAGCACCGTTTCTTTACCAAGATAGTGGTCGATACGGTAAACCTGGCACTCCTCGAAGTATTCGCCAACCTGATCATTGATTTCCTGCGAGGTCGCCAGCGACGTCCCCAGCGGTTTCTCCATGACTACGCGTGCCGGTTTAGCATTCAGTTTTGCCTCGCCAAGCCCTTTGCAAATTGCGCCAAAAGTGCTGGGCGGCATGGCAAAGTAGTTAATGGTGATACGATTTTTTTGATCCAGCATCGCGCCGAGACGGCTGAATGCAGCAGTGTCATTGACATC  >  NC_000913/1935755‑1936041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑