Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,067,130 T→C 5.2% A193A (GCA→GCG wrbA ← NAD(P)H:quinone oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,067,1300TC 5.2% 240.8 / 2.8 77A193A (GCA→GCGwrbANAD(P)H:quinone oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (35/38);  new base C (2/2);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

TTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTG  >  NC_000913/1066983‑1067278
                                                                                                                                                   |                                                                                                                                                    
tttCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTg                                                                                                                                                     >  7:339196/1‑149 (MQ=255)
       gtACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGAc                                                                                                                                               <  1:196210/148‑1 (MQ=255)
            cGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGCTGCCCATTCACCGCCGTGATCAGCTTTCGCTTCTTGAGTTGGCATGCGGATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGa                                                                                                                                         <  4:14420/149‑1 (MQ=255)
                  cTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTAAACTGCCAGACCTGCGACATAtt                                                                                                                                   <  8:37822/149‑1 (MQ=255)
                   ttCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTc                                                                                                                                  >  4:158259/1‑149 (MQ=255)
                     cGAAAATTGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCACATTCACGTACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCTACATATTcc                                                                                                                                 <  8:345386/148‑1 (MQ=255)
                      gAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTTAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTcccc                                                                                                                               <  7:1784/149‑1 (MQ=255)
                       aaaaTGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCt                                                                                                                              <  5:356855/149‑1 (MQ=255)
                               cTCGGATATTTCCGGCGACGTATTGCTCAGAGATGCCAATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAAc                                                                                                                       <  2:137603/148‑1 (MQ=255)
                                 cGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAg                                                                                                                    <  6:153583/149‑1 (MQ=255)
                                 cGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAg                                                                                                                    >  7:230931/1‑149 (MQ=255)
                                  ggCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGa                                                                                                                     <  4:169180/147‑1 (MQ=255)
                                  ggCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGa                                                                                                                     <  7:340642/147‑1 (MQ=255)
                                  ggCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAg                                                                                                                    <  8:230931/148‑1 (MQ=255)
                                           cGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACa                                                                                                           <  2:40891/148‑1 (MQ=255)
                                           cGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAg                                                                                                          >  2:8347/1‑149 (MQ=255)
                                             gCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGtt                                                                                                        <  6:287345/149‑1 (MQ=255)
                                                 cGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGttcttc                                                                                                    <  3:140466/149‑1 (MQ=255)
                                                   tATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCct                                                                                                  >  1:252193/1‑149 (MQ=255)
                                                     ttGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCctg                                                                                                 <  5:243236/148‑1 (MQ=255)
                                                        cgcAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCctggct                                                                                              <  4:147218/148‑1 (MQ=255)
                                                               aTGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAAt                                                                                                                >  7:107218/1‑123 (MQ=255)
                                                               aTGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAAt                                                                                                                <  8:107218/123‑1 (MQ=255)
                                                                tGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGc                                                                                      <  8:339196/148‑1 (MQ=255)
                                                                 gCCCATTCACCGACGTTATGAGCTTTAGCTTCTTGAGTTGGCATGCGGATCCTCCTGTTGAAGATTAGCCGTTAAGGTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGt                                                                                    <  5:73048/149‑1 (MQ=255)
                                                                  cccATTCACCGACGTGATTAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGt                                                                                    <  1:137132/148‑1 (MQ=255)
                                                                  cccATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGt                                                                                    <  1:8347/148‑1 (MQ=255)
                                                                   ccATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTg                                                                                   >  8:211877/1‑148 (MQ=255)
                                                                     aTTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCGGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                >  6:68432/1‑149 (MQ=255)
                                                                     aTTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                <  3:158259/149‑1 (MQ=255)
                                                                      ttCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAcc                                                                               >  8:195723/1‑149 (MQ=255)
                                                                      ttCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACCGCCAGACCTGCGACATATTCCCCTTGATAACGAGCaa                                                                                                                 >  4:115893/1‑115 (MQ=255)
                                                                      ttCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACCGCCAGACCTGCGACATATTCCCCTTGATAACGAGCaa                                                                                                                 >  4:120892/1‑115 (MQ=255)
                                                                      ttCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACCGCCAGACCTGCGACATATTCCCCTTGATAACGAGCaa                                                                                                                 <  3:115893/115‑1 (MQ=255)
                                                                      ttCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACCGCCAGACCTGCGACATATTCCCCTTGATAACGAGCaa                                                                                                                 <  3:120892/115‑1 (MQ=255)
                                                                         aCCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGt                                                                             >  7:146283/1‑148 (MQ=255)
                                                                                tgatgaGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCt                                                                     >  2:196136/1‑149 (MQ=255)
                                                                                    gaGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGa                                                                 >  8:347732/1‑149 (MQ=255)
                                                                                     aGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGAt                                                                <  1:263632/149‑1 (MQ=255)
                                                                                       cTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGAtgg                                                              >  4:328918/1‑149 (MQ=255)
                                                                                            gCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGCCAGTTCTTCCTGGCTGGGCTGGGGTGAACCGTCACCGCCTGCGATGGTGGtt                                                         <  7:85634/149‑1 (MQ=255)
                                                                                             cttcttGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTg                                                        >  3:208044/1‑149 (MQ=255)
                                                                                              ttcttGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                >  1:220452/1‑124 (MQ=255)
                                                                                              ttcttGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                <  2:220452/124‑1 (MQ=255)
                                                                                              ttcttGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTgc                                                       >  7:352736/1‑149 (MQ=255)
                                                                                                         gCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCg                                            >  8:133355/1‑149 (MQ=255)
                                                                                                           aTGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTa                                          >  4:82756/1‑149 (MQ=255)
                                                                                                              cGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTAccg                                       >  6:307244/1‑149 (MQ=255)
                                                                                                                  tcctccTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACcgccgc                                   <  1:95202/149‑1 (MQ=255)
                                                                                                                     tcctGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTgcg                                                      <  7:270101/127‑1 (MQ=255)
                                                                                                                     tcctGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTgcg                                                      >  8:270101/1‑127 (MQ=255)
                                                                                                                     tcctGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGaa                                >  6:23363/1‑149 (MQ=255)
                                                                                                                      cctGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAAc                               >  8:354060/1‑149 (MQ=255)
                                                                                                                         gTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCtg                            >  7:183893/1‑149 (MQ=255)
                                                                                                                          ttGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCtgt                           >  8:224780/1‑149 (MQ=255)
                                                                                                                          ttGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCtg                            <  5:23363/148‑1 (MQ=255)
                                                                                                                            gAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCtgtg                          >  5:141279/1‑148 (MQ=255)
                                                                                                                            gAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTACCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCtgtg                          >  3:233293/1‑148 (MQ=255)
                                                                                                                              aGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGaaa                       >  3:243076/1‑149 (MQ=255)
                                                                                                                               gATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAAc                      <  4:243076/149‑1 (MQ=255)
                                                                                                                                   aGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTc                   <  8:146283/148‑1 (MQ=255)
                                                                                                                                   aGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTc                   <  3:82756/148‑1 (MQ=255)
                                                                                                                                    gcggTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCa                  <  5:68432/145‑1 (MQ=255)
                                                                                                                                     ccGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCaaa                >  6:337864/1‑149 (MQ=255)
                                                                                                                                       gTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAaat               >  2:7672/1‑148 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGaa                                <  3:45219/126‑1 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGaa                                >  4:45219/1‑126 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTcc         <  6:141279/149‑1 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTcc         <  3:80523/149‑1 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTcc         >  8:118474/1‑149 (MQ=255)
                                                                                                                                            gTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTc          >  4:80523/1‑148 (MQ=255)
                                                                                                                                             tttAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTGCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCct        <  7:211877/149‑1 (MQ=255)
                                                                                                                                              ttAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctg       >  5:73591/1‑149 (MQ=255)
                                                                                                                                                aaCTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctgcg     >  5:112642/1‑149 (MQ=255)
                                                                                                                                                aaCTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctgcg     >  5:54322/1‑149 (MQ=255)
                                                                                                                                                 aCTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctgcg     <  3:328918/148‑1 (MQ=255)
                                                                                                                                                  cTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctgcgct   <  1:7672/149‑1 (MQ=255)
                                                                                                                                                   tGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                <  2:150522/71‑1 (MQ=255)
                                                                                                                                                   tGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAAc                                                                                >  1:150522/1‑71 (MQ=255)
                                                                                                                                                   tGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCctgcgctg  >  3:303519/1‑149 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TTTCGTCGTACCCGGCGACTTCGAAAATGGCCTCGGCTATTTCCGGCGACGTATTGCGCAGAGATGCCCATTCACCGACGTGATGAGCTTTCGCTTCTTGAGTTGGCATGCGTATCCTCCTGTTGAAGATTAGCCGTTAAGTTTAACTGCCAGACCTGCGACATATTCCCCTTGATAACGAGCAATAGACAGTTCTTCCTGGCTGGGCTGGCGTGAACCGTCACCGCCTGCGATGGTGGTTGCGCCGTACGGCGTACCGCCGCGAACCTGTGAAACGTCAAATAATTCCTGCGCTG  >  NC_000913/1066983‑1067278

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: