Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 933,515 T→C 7.0% W98R (TGG→CGG)  ftsK → DNA translocase at septal ring sorting daughter chromsomes

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913933,5150TC 7.0% 217.0 / 2.4 74W98R (TGG→CGG) ftsKDNA translocase at septal ring sorting daughter chromsomes
Reads supporting (aligned to +/- strand):  ref base T (34/33);  new base C (3/2);  total (38/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGG  >  NC_000913/933372‑933663
                                                                                                                                               |                                                                                                                                                    
gCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGt                                                                                                                                                 >  1:201989/1‑149 (MQ=255)
    gTCGCAATCGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGGTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGtcat                                                                                                                                              >  2:79426/1‑148 (MQ=255)
    gTCGCAAACGGCCTGGCATGAACGTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTGCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGtcat                                                                                                                                              <  3:28221/148‑1 (MQ=255)
    gTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGtcat                                                                                                                                              <  1:140863/148‑1 (MQ=255)
    gTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGtca                                                                                                                                               <  1:64693/147‑1 (MQ=255)
     tCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGtcatca                                                                                                                                            <  2:57373/149‑1 (MQ=255)
       gCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGt                                                                                                                                          >  6:232558/1‑149 (MQ=255)
       gCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTACCGTCATTATTGTCGGCGGTTGGTGGTTTGCCGGGCGTCCTCAGt                                                                                                                                          >  4:89413/1‑149 (MQ=255)
         aaaCGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTc                                                                                                                                         >  7:220950/1‑148 (MQ=255)
         aaaCGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTATATTTTTGGCGTGATGGCTTACACCATTCCCGTCAGTATTGTCGGCGGTTGTTGGTTTGCCCGGCGTCATCAGTcc                                                                                                                                        >  8:332796/1‑149 (MQ=255)
         aaaCGGCCTGGCATGAACCTATCAATAATTTAGGTGGGAGGCCCGGTGCGTGGTTGGAAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCTGCGGTTGTTGGTTTGCCCGGCGTCATCAGTcc                                                                                                                                        >  8:337769/1‑149 (MQ=255)
               ccTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGcgac                                                                                                                                  <  2:373136/149‑1 (MQ=255)
               ccTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGcgac                                                                                                                                  <  5:232558/149‑1 (MQ=255)
                       gAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACAt                                                                                                                          >  3:194200/1‑149 (MQ=255)
                        aaCCTATCCATAATTTAGGTGGGATGCCCTGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACAt                                                                                                                          <  8:220950/148‑1 (MQ=255)
                        aaCCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACAt                                                                                                                          <  6:119523/148‑1 (MQ=255)
                        aaCCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTAGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACAtt                                                                                                                         <  5:321455/149‑1 (MQ=255)
                              tCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCTGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGattat                                                                                                                   >  3:128727/1‑149 (MQ=255)
                                cATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTAttt                                                                                                                 >  6:229133/1‑149 (MQ=255)
                                 aTAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTAtttt                                                                                                                <  6:272672/149‑1 (MQ=255)
                                 aTAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTAtttt                                                                                                                >  4:125161/1‑149 (MQ=255)
                                  tAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTg                                                                                                               >  2:120318/1‑149 (MQ=255)
                                    aTTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGAGGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGc                                                                                                              <  2:3438/148‑1 (MQ=255)
                                    aTTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTGATTTATTTTTGGCGTGAGGGCTTACCCCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATATTGcc                                                                                                             <  5:236676/149‑1 (MQ=255)
                                       tAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGt                                                                                                           <  1:79426/148‑1 (MQ=255)
                                        aGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGttt                                                                                                         >  6:26704/1‑149 (MQ=255)
                                         ggggggATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTc                                                                                                        <  3:245179/146‑1 (MQ=255)
                                         ggTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTc                                                                                                        >  6:228589/1‑149 (MQ=255)
                                          gTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCg                                                                                                       >  4:7553/1‑149 (MQ=255)
                                              gATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTAc                                                                                                   <  3:125161/149‑1 (MQ=255)
                                                   ccGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGcat                                                                                               >  3:31282/1‑148 (MQ=255)
                                                   ccGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCCGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGcc                                                                                                             >  7:334167/1‑134 (MQ=255)
                                                   ccGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCAGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGcc                                                                                                             <  8:334167/134‑1 (MQ=255)
                                                      gTGCGTGGTTGGCAGATACGATGTTCTTTATTTTTGTCGTGAGGGCGTACACCATTCCCGTCATTATTGTAGGCGGTTGGTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCGGTTTCGCTACGCATCAtt                                                                                           <  3:89413/149‑1 (MQ=255)
                                                       tGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTg                                                                                          <  3:277028/149‑1 (MQ=255)
                                                         cGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGGTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGc                                                                                        <  4:194200/149‑1 (MQ=255)
                                                           tGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTAtttt                                                                                                                >  5:239237/1‑123 (MQ=255)
                                                           tGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTAtttt                                                                                                                <  6:239237/123‑1 (MQ=255)
                                                           gtgTTGGCAGATACGCTGTTCTTTATTTTTGGAGTGAGGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGt                                                                                      <  4:28179/147‑1 (MQ=255)
                                                                ggCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTgg                                                                                 >  4:26937/1‑149 (MQ=255)
                                                                        cGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATc                                                                         >  7:265295/1‑149 (MQ=255)
                                                                            gTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTa                                                                     >  8:349527/1‑149 (MQ=255)
                                                                                tttATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTAcctc                                                                 >  3:195690/1‑149 (MQ=255)
                                                                                 ttATTTTTGGCTTGATGGATTACAACATTCACTTCATGATTGTCGGCGTTGGTTGGTTTGCCTGTCGTCATCCGTCCCGCGACGAATACATTGATTATTTTTCCTTTTCGCTCCGCATCATGGGCGTGTTGTCGCTCCTCCTTAcntaa                                                                >  5:34406/1‑147 (MQ=255)
                                                                                 ttATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGACCAGCGACGAATACATTGATTATTTTGCCGTTTCGATACGCATCATTGGCGTTTTGGCGCTCATCCTTAcctcc                                                                >  5:39253/1‑149 (MQ=255)
                                                                                  tATTTTTGGCGTGATGGCTTACACCATTCCTGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGAATCATTGGCGTTTTGGCGCTCATCCTTAcctc                                                                 <  4:128727/147‑1 (MQ=255)
                                                                                      tttGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCtgtg                                                            <  5:26704/148‑1 (MQ=255)
                                                                                            gggATGGCGTACACCATTCCCGTCATTCTTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCATTCCATCGTCGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTATGGCGCTCATCCTTACCTCCTGTGGTCTggc                                                     <  2:102997/147‑1 (MQ=255)
                                                                                             tGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTAc                                                                    >  7:156680/1‑133 (MQ=255)
                                                                                                   cTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGtttt                                                                                   >  8:267114/1‑112 (MQ=255)
                                                                                                   cTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGtttt                                                                                   <  7:267114/112‑1 (MQ=255)
                                                                                                   cTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAAt                                               >  4:286996/1‑148 (MQ=255)
                                                                                                          cATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCt                                        >  4:110427/1‑148 (MQ=255)
                                                                                                          cATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCt                                        >  6:317194/1‑148 (MQ=255)
                                                                                                           aTTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGa                                      <  8:265295/149‑1 (MQ=255)
                                                                                                            ttCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGAc                                     >  3:68458/1‑149 (MQ=255)
                                                                                                             tCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGAc                                     >  2:115002/1‑148 (MQ=255)
                                                                                                                 gTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGatat                                <  1:120318/149‑1 (MQ=255)
                                                                                                                   cATTATTGTCGGCGGTTGTTGTTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGAATCAATGGCGTTTTGGCGATCATCATTACCTCCTGTGGTCTGTCGGAAATCAACGCTGACGatatat                              >  4:56037/1‑147 (MQ=255)
                                                                                                                    attattGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCt                              <  4:31282/148‑1 (MQ=255)
                                                                                                                    attattGTCGGCGGTTGTTGGTTTGCCTGGCGTCATAAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGTCTCTCATCCTTACCTCCAGTGGTCGGGCGGCAATCAACGCTTACGATATATg                             >  8:101256/1‑149 (MQ=255)
                                                                                                                     ttattGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTgg                            <  8:74988/149‑1 (MQ=255)
                                                                                                                      tattGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTAc                                                                    >  8:9368/1‑108 (MQ=255)
                                                                                                                      tattGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTAc                                                                    <  7:9368/108‑1 (MQ=255)
                                                                                                                           tCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTAtttt                      >  5:270767/1‑149 (MQ=255)
                                                                                                                           tCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTAttt                       <  4:68458/148‑1 (MQ=255)
                                                                                                                           tCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTAGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTAtttt                      <  5:229133/149‑1 (MQ=255)
                                                                                                                           tCGGCGGTTGTTGGTTTGCCCGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTAGGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTAtttt                      <  7:332796/149‑1 (MQ=255)
                                                                                                                           tCGGAGTTTGTTGGTTTGCCCGGCGTCATCATTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGTCGTTTTTGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTAtttt                      <  7:337769/149‑1 (MQ=255)
                                                                                                                                  ttgttgGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCg               >  5:341271/1‑149 (MQ=255)
                                                                                                                                     ttgGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGt             <  4:195690/148‑1 (MQ=255)
                                                                                                                                        gTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCg         >  3:312501/1‑149 (MQ=255)
                                                                                                                                             ccTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCAtt    <  5:228589/149‑1 (MQ=255)
                                                                                                                                              cTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTg   >  8:48931/1‑149 (MQ=255)
                                                                                                                                               tGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCTATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGTCGTCATTgg  >  6:132980/1‑149 (MQ=255)
                                                                                                                                               tGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTgg  >  1:209596/1‑149 (MQ=255)
                                                                                                                                               |                                                                                                                                                    
GCTGGTCGCAAACGGCCTGGCATGAACCTATCCATAATTTAGGTGGGATGCCCGGTGCGTGGTTGGCAGATACGCTGTTCTTTATTTTTGGCGTGATGGCTTACACCATTCCCGTCATTATTGTCGGCGGTTGTTGGTTTGCCTGGCGTCATCAGTCCAGCGACGAATACATTGATTATTTTGCCGTTTCGCTACGCATCATTGGCGTTTTGGCGCTCATCCTTACCTCCTGTGGTCTGGCGGCAATCAACGCTGACGATATCTGGTATTTTGCCTCCGGTGGCGTCATTGG  >  NC_000913/933372‑933663

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑