Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,159,109	A→G	N414S (AAC→AGC)	ptsG →	fused glucose‑specific PTS enzymes: IIB component/IIC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,159,109	0	A	G	100.0%	111.2 / NA	32	N414S (AAC→AGC)	ptsG	fused glucose‑specific PTS enzymes: IIB component/IIC component
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/17);  total (15/17)

ATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAA  >  NC_000913/1158964‑1159254                                                                                                                                                  |                                                                                                                                                  aTTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCAt                                                                                                                                                <  1:49233/149‑1 (MQ=255)           acacCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTc                                                                                                                                      >  1:65974/1‑149 (MQ=255)                         gTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAc                                                                                                                        <  2:1933/149‑1 (MQ=255)                           gCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTAccc                                                                                                                      >  3:85216/1‑149 (MQ=255)                               aTTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGt                                                                                                                    <  4:85216/147‑1 (MQ=255)                                       aCTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCa                                                                                                          <  1:15139/149‑1 (MQ=255)                                               tGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                                  >  7:15370/1‑149 (MQ=255)                                                 aaaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCt                                                                                                  <  2:65974/147‑1 (MQ=255)                                                  aaaCGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               <  3:87423/149‑1 (MQ=255)                                                                        gACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCAGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAggccg                                                                         >  4:100042/1‑149 (MQ=255)                                                                             aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               >  4:56829/1‑122 (MQ=255)                                                                             aaGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAt                                                                                               <  3:56829/122‑1 (MQ=255)                                                                                  gCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                             >  4:13394/1‑119 (MQ=255)                                                                                  gCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGAtgt                                                                                             <  3:13394/119‑1 (MQ=255)                                                                                              ggTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGc                                                   >  3:72795/1‑149 (MQ=255)                                                                                              ggTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGc                                                   >  3:85251/1‑149 (MQ=255)                                                                                               gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                   <  6:7435/68‑1 (MQ=255)                                                                                               gTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGAc                                                                                                                                   >  5:7435/1‑68 (MQ=255)                                                                                                              cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTgg                                                          >  5:125164/1‑126 (MQ=255)                                                                                                              cACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTgg                                                          <  6:125164/126‑1 (MQ=255)                                                                                                               aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                         <  8:69221/142‑1 (MQ=255)                                                                                                               aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTg                                         >  7:69221/1‑142 (MQ=255)                                                                                                               aCCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  <  8:15370/149‑1 (MQ=255)                                                                                                                          ttGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  <  1:113371/138‑1 (MQ=255)                                                                                                                          ttGCTGCATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTg                                  >  2:113371/1‑138 (MQ=255)                                                                                                                              tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGaaa                                                              >  5:32138/1‑106 (MQ=255)                                                                                                                              tgcATTTGGTGGTAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGaaa                                                              <  6:32138/106‑1 (MQ=255)                                                                                                                                       tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCgg                                                 <  4:79967/110‑1 (MQ=255)                                                                                                                                       tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg          <  4:85251/149‑1 (MQ=255)                                                                                                                                       tggtAAAGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCg          <  4:72795/149‑1 (MQ=255)                                                                                                                                            aaGAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTAc      >  8:92849/1‑148 (MQ=255)                                                                                                                                              gAAAGCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTa    >  2:117224/1‑148 (MQ=255)                                                                                                                                               aaagCATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTaaa  >  2:41741/1‑149 (MQ=255)                                                                                                                                                  |                                                                                                                                                  ATTGTTTACTACACCATCTTCCGCGTGCTGATTAAAGCACTGGATCTGAAAACGCCGGGTCGTGAAGACGCGACTGAAGATGCAAAAGCGACAGGTACCAGCGAAATGGCACCGGCTCTGGTTGCTGCATTTGGTGGTAAAGAAAACATTACTAACCTCGACGCATGTATTACCCGTCTGCGCGTCAGCGTTGCTGATGTGTCTAAAGTGGATCAGGCCGGCCTGAAGAAACTGGGCGCAGCGGGCGTAGTGGTTGCTGGTTCTGGTGTTCAGGCGATTTTCGGTACTAAA  >  NC_000913/1158964‑1159254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑