Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	754,103	G→T	F122L (TTC→TTA)	gltA ←	citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	754,103	0	G	T	100.0%	61.1 / NA	19	F122L (TTC→TTA)	gltA	citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (9/10);  total (9/10)

GCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGA  >  NC_000913/753961‑754248                                                                                                                                               |                                                                                                                                                  gCATTTTCGACAGCAGGCGGAACGCGGCAAGTTCACGGTGACGAGGATTGTTAACATCCAGCGAGGCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGa                                                                                                                                             <  7:53917/149‑1 (MQ=255)               agGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTc                                                                                                                               <  3:115318/149‑1 (MQ=255)                                         aCGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCg                                                                                                     >  3:80743/1‑149 (MQ=255)                                                           cAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                                                              >  8:119782/1‑138 (MQ=255)                                                           cAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                                                              <  7:119782/138‑1 (MQ=255)                                                             gagAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACgg                                                                                                                                          <  8:91877/90‑1 (MQ=255)                                                                   cGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTcc                                                                           <  4:80743/149‑1 (MQ=255)                                                                     tGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCt                                                                          >  8:70678/1‑148 (MQ=255)                                                                                         gcCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTca                                                     <  7:70678/149‑1 (MQ=255)                                                                                                       acaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTc                                                                                        <  1:101208/100‑1 (MQ=255)                                                                                                       acaTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTc                                                                                        >  2:101208/1‑100 (MQ=255)                                                                                                         aTGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAAc                                     >  3:50405/1‑149 (MQ=255)                                                                                                                ccATTGGATGCGAGTCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCa                                <  4:50405/147‑1 (MQ=255)                                                                                                                              tCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCg                  >  1:621/1‑147 (MQ=255)                                                                                                                              tCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCg                  <  2:621/147‑1 (MQ=255)                                                                                                                              tCGCGACGGAAAGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGt                >  3:4793/1‑149 (MQ=255)                                                                                                                                         aGCATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgat     <  4:4793/149‑1 (MQ=255)                                                                                                                                           cATGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatc    >  4:19331/1‑148 (MQ=255)                                                                                                                                            aTGTAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga  >  4:58027/1‑149 (MQ=255)                                                                                                                                               |                                                                                                                                                  GCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGGTGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGCGAGTCGCGACGGAAAGCATGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGA  >  NC_000913/753961‑754248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑