Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,305,0860CG25.0% 50.1 / 18.0 28intergenic (+693/+22)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base C (10/11);  new base G (3/4);  total (13/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.07e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTG  >  NC_000913/2304939‑2305222
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gcgcGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATAc                                                                                                                                          >  5:131711/1‑148 (MQ=255)
         aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGa                                                                                                                                 <  1:90076/148‑1 (MQ=255)
         aTCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                                                                                                                >  8:107533/1‑149 (MQ=255)
             ggCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTGGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTgg                                                                                                                            <  4:29652/149‑1 (MQ=255)
              gCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                           <  5:29898/149‑1 (MQ=255)
                                tGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCaa                                                                                                          >  3:64437/1‑148 (MQ=255)
                                   tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                           <  1:106970/128‑1 (MQ=255)
                                   tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                           >  2:106970/1‑128 (MQ=255)
                                      aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc                                                                                                   <  6:131711/149‑1 (MQ=255)
                                        cccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgt                                                                                                                              >  4:35417/1‑120 (MQ=255)
                                        cccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgt                                                                                                                              <  3:35417/120‑1 (MQ=255)
                                           gTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                           <  5:105309/120‑1 (MQ=255)
                                           gTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGc                                                                                                                           >  6:105309/1‑120 (MQ=255)
                                                ttGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTa                                                                                                                        <  5:115586/118‑1 (MQ=255)
                                                ttGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGATGTGGCGTa                                                                                                                        >  6:115586/1‑118 (MQ=255)
                                                  ggATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCttttt                                                                                        <  7:107533/148‑1 (MQ=255)
                                                                         cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt                                                                                         >  3:8806/1‑124 (MQ=255)
                                                                         cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt                                                                                         <  4:8806/124‑1 (MQ=255)
                                                                         cAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCg                                                                <  4:64437/149‑1 (MQ=255)
                                                                              tCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTc                                                           >  4:113728/1‑149 (MQ=255)
                                                                                                                        ttATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAAccc                  >  5:61766/1‑148 (MQ=255)
                                                                                                                                    gCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTg     >  7:37924/1‑149 (MQ=255)
                                                                                                                                      cacaTCCTTCATACCATGCCGGATGTGTCGTATCATTACAACGCAATATCCGCCAGTTCTTTTTGAAGGGTTTGCGGTTTCAACTGCGGTTTCGGCGTACTATct                                               <  5:33180/105‑1 (MQ=255)
                                                                                                                                      cacaTCCGGCATACCATGCCAGATGTGGCGTAACAATACAACGCAAAATCCCCCACGACTTTTTGAACGCTTACCGGTTTCAACAGCGCTATCGGCGTACTATct                                               >  6:33180/1‑105 (MQ=255)
                                                                                                                                       acaTCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGgtg  <  8:37924/149‑1 (MQ=255)
                                                                                                                                          tCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAg                          <  6:112784/122‑1 (MQ=255)
                                                                                                                                          tCCGGCATGGTATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAg                          >  5:112784/1‑122 (MQ=255)
                                                                                                                                              gCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAg               <  6:117828/129‑1 (MQ=255)
                                                                                                                                              gCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAg               >  5:117828/1‑129 (MQ=255)
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GCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTGAACGGGTTGCGGTTTCAACTGCGGTTTCGGCGTACTATCTGCTGCCTGCGGCTTGTCGTAGTTCAACCCCAGCACTTCGCTGGTG  >  NC_000913/2304939‑2305222

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑