Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,771,708	IS3 (–) +4 bp :: +TC	coding (34‑37/363 nt)	yibI ←	DUF3302 family inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	315229 =	NA (NA)	44 (1.550) +TC	33/248	0.1	100%	noncoding (1/1255 nt)	IS3	repeat region
	?	NC_000913	3771708 =	0 (0.000)					coding (37/363 nt)	yibI	DUF3302 family inner membrane protein
*	?	NC_000913	= 2171428	NA (NA)	31 (1.070)	30/252	0.1	100%	noncoding (1258/1258 nt)	IS3	repeat region
	?	NC_000913	= 3771711	0 (0.000)					coding (34/363 nt)	yibI	DUF3302 family inner membrane protein

ACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171297‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT  <  NC_000913/3771711‑3771564                                                                                                                                                                                                                                                                                          ACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATAATACGTGGGTAGGATCAATCTTCGTCTTTCTGGT                                                                                                                                     <  4:85183/149‑1       TTTAATTATATAGAAAGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGACCTCAGTCCGGAACAATTTTCAAACAACAACCTCGCTTAGGCCTGTGTCCATATTACGTTGGTAGGATCAATCTTCGACTATCTGATGATATT                                                                                                                               >  4:21312/1‑149         TAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCT                                                                                                                                                  >  1:122384/1‑128         TAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCT                                                                                                                                                  <  2:122384/128‑1                      TGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGC                                                                                                                <  3:21312/149‑1                      TGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATC                                                                                                                  >  1:85228/1‑147                      TGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATC                                                                                                                  <  2:85228/147‑1                           TTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAGTCATCGCGATA                                                                                                            >  7:13049/1‑148                               AATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATAAATCTTCGTCTTTCTGTTGATTTTTTATGGAATCATCGCGATAAATG                                                                                                        >  8:87102/1‑148                                         GGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGT                                                                                                                                     >  8:85901/1‑109                                         GGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGT                                                                                                                                     <  7:85901/109‑1                                                  GTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGACTCATCGCGATACATGACATCCCTTATCTGATTGCC                                                                                    <  8:13049/149‑1                                                                        GGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTC                                                                                                                                             >  6:61888/1‑70                                                                        GGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTC                                                                                                                                             <  5:61888/70‑1                                                                         GAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGC                                                             >  2:108983/1‑149                                                                               TTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGC                                                       >  3:58041/1‑149                                                                                    AAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTC                                                  <  1:108983/149‑1                                                                                       CAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGAACAATCTTCGTATTTCTGGTGATTTTTTATGGAATCATCGAGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACAATCCCCATGCCGACGCTATTCATA                                               <  7:87102/149‑1                                                                                           AACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCC                                                                                                  <  1:21435/94‑1                                                                                           AACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCC                                                                                                  >  2:21435/1‑94                                                                                                  CTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCG                                                                                                                 >  7:50502/1‑72                                                                                                  CTTAGGCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCG                                                                                                                 <  8:50502/72‑1                                                                                                       GCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGC                                                             >  4:118691/1‑119                                                                                                       GCCTGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGC                                                             <  3:118691/119‑1                                                                                                          TGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC                                           >  7:43061/1‑134                                                                                                          TGTGTCCATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGC                                           <  8:43061/134‑1                                                                                                                CATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGC                                       <  4:31742/132‑1                                                                                                                CATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGC                                       >  3:31742/1‑132                                                                                                                 ATATTACGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACT                     >  1:44148/1‑149                                                                                                                       CGTGGGTAGGATCAATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCA                <  2:44148/148‑1                                                                                                                                    AATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT  <  4:58041/149‑1                                                                                                                                                                                                                                                                                          ACGGTGTTTAATTATATCGAATGTGATTACAATCGGTGGCGGCGGCACAGTTGGTGTGGCGGCCTCAGTCCGGAACAATTTGAAAACAAGAACCTCGCTTAGGCCTGTGTCCATATTACGTGGGTAGGATCA‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2171297‑2171428 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ATCTTCGTCTTTCTGGTGATTTTTTATGGAATCATCGCGATACATGACATCCCTTATCTGATTGCCAAAAAGCGCAACCATCCCCATGCCGACGCTATTCATACGGCGGGCTGGGTGAGCCTGTTTACTCTGCATGTTATCTGGCCGT  <  NC_000913/3771711‑3771564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.