Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,668,470	G→A	S32L (TCG→TTG)	mlc ←	glucosamine anaerobic growth regulon transcriptional repressor; autorepressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,668,470	0	G	A	100.0%	354.0 / NA	97	S32L (TCG→TTG)	mlc	glucosamine anaerobic growth regulon transcriptional repressor; autorepressor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (50/47);  total (50/47)

CCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGG  >  NC_000913/1668327‑1668612                                                                                                                                                |                                                                                                                                               ccaccaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACTGGAAAGATCGATACGCAAGACt                                                                                                                                           >  6:172044/1‑149 (MQ=255) ccaccaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACt                                                                                                                                           <  1:139550/149‑1 (MQ=255)    ccaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGa                                                                                                                                        <  2:460485/149‑1 (MQ=255)    ccaGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGa                                                                                                                                        <  6:142220/149‑1 (MQ=255)          ccACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGGCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGc                                                                                                                                  <  2:355489/149‑1 (MQ=255)          ccACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGc                                                                                                                                  <  1:169632/149‑1 (MQ=255)           cACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGATCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCt                                                                                                                                 >  1:14907/1‑149 (MQ=255)            aCCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTg                                                                                                                                <  1:210137/149‑1 (MQ=255)             ccgccgGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGa                                                                                                                               <  8:11949/149‑1 (MQ=255)               gccgGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGat                                                                                                                              >  6:343039/1‑148 (MQ=255)                ccgGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGatca                                                                                                                            <  1:275365/149‑1 (MQ=255)                        ccACGTTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGc                                                                                                                     <  1:135685/148‑1 (MQ=255)                        ccACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTgg                                                                                                                                         <  8:382846/128‑1 (MQ=255)                        ccACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTgg                                                                                                                                         >  7:382846/1‑128 (MQ=255)                        ccACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGc                                                                                                                     >  2:128999/1‑148 (MQ=255)                        ccACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGc                                                                                                                     >  3:203060/1‑148 (MQ=255)                        ccACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGc                                                                                                                     <  1:128999/148‑1 (MQ=255)                        caaCGGTTCCCCTCTTATTTGAATTCCAGCTCTTGCACCAGGTCTGCTACGAGCATCTCACGGACAAATATAGTTATACTGGCAGGAGCCAGTTGCGCAAGACGGGAAATATCGATACGCAAGACTGGACCAAGCTGatcaatcacac                                                                                                                     >  2:135685/3‑145 (MQ=255)                           cGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGAt                                                                                                                  >  5:276016/1‑148 (MQ=255)                              ttCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAAc                                                                                                              >  1:440554/1‑149 (MQ=255)                                      ttcttTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCcg                                                                                                      >  8:279568/1‑149 (MQ=255)                                      ttcttTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCcg                                                                                                      >  3:71630/1‑149 (MQ=255)                                         tttGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGt                                                                                                   >  2:276485/1‑149 (MQ=255)                                           tGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTg                                                                                                 >  1:28277/1‑149 (MQ=255)                                            gATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTgg                                                                                                >  1:485875/1‑149 (MQ=255)                                             aTTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGt                                                                                               <  3:457948/149‑1 (MQ=255)                                              tttCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTc                                                                                              >  2:90572/1‑149 (MQ=255)                                                 cccgCTCTTGCACCCGGTGTGCGTCGAGCCTCTCACGGACAATTGTAGGGATACTGGCAGGAGCCAGTTGCGCCAGACGGGACCGATCGATACGCAAGACTGTACCAAGCTGATCAATCAGGCGATAAACCGCGccc                                                                                                       <  4:109094/134‑1 (MQ=255)                                                 ccAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGc                                                                                           >  2:261168/1‑149 (MQ=255)                                                  cAGCTCTTGCACCAGGTGTGCTTCGCGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCt                                                                                          >  2:100512/1‑149 (MQ=255)                                                          gCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGa                                                                                  >  3:401065/1‑149 (MQ=255)                                                          gCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGa                                                                                  >  3:368139/1‑149 (MQ=255)                                                           cACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTTGTCTGCTTTATTTGa                                                                                  <  4:203060/148‑1 (MQ=255)                                                            aCCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGGTCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGa                                                                                                                   >  4:48650/1‑114 (MQ=255)                                                            aCCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGGTCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGa                                                                                                                   <  3:48650/114‑1 (MQ=255)                                                            aCCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGatcaat                                                                                                                          >  6:22748/1‑107 (MQ=255)                                                            aCCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGatcaat                                                                                                                          <  5:22748/107‑1 (MQ=255)                                                            aCCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGAt                                                                                 >  8:146900/1‑148 (MQ=255)                                                             ccAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCa                                                                               <  6:155947/149‑1 (MQ=255)                                                                   gtgCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGc                                                                         >  8:110689/1‑149 (MQ=255)                                                                   gtgCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGc                                                                         >  6:190284/1‑149 (MQ=255)                                                                     gCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGcc                                                                        >  8:44362/1‑148 (MQ=255)                                                                      cTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGcc                                                                        >  2:134848/1‑147 (MQ=255)                                                                          gAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGc                                                                   <  7:110689/148‑1 (MQ=255)                                                                             cATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGt                                                               >  4:321/1‑149 (MQ=255)                                                                              aTCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGtt                                                              <  4:99156/149‑1 (MQ=255)                                                                               tctcACGGACAATGTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGTCGATAAACCGCGCCCGCGATGGTCTGCTTTATTTGATCAATGAGCCCAGGCTGCttt                                                             >  6:191436/1‑149 (MQ=255)                                                                                ctcACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGtttt                                                            >  7:38677/1‑149 (MQ=255)                                                                                ctcACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGtttt                                                            <  1:336667/149‑1 (MQ=255)                                                                                 tcACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTAttt                                                                                    <  6:40633/124‑1 (MQ=255)                                                                                 tcACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTAttt                                                                                    >  5:40633/1‑124 (MQ=255)                                                                                  cACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGtt                                                              <  2:57715/145‑1 (MQ=255)                                                                                  cACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGtt                                                              >  1:57715/1‑145 (MQ=255)                                                                                   aCGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGttt                                                             <  5:291024/145‑1 (MQ=255)                                                                                   aCGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTc                                                           <  1:134848/147‑1 (MQ=255)                                                                                   aCGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCa                                                          <  2:327605/148‑1 (MQ=255)                                                                                   aCGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAg                                                         >  2:308580/1‑149 (MQ=255)                                                                                    cGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGc                                                        <  1:308580/149‑1 (MQ=255)                                                                                     ggACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGc                                                        >  4:415615/1‑148 (MQ=255)                                                                                       aCAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAac                                                     <  4:368139/149‑1 (MQ=255)                                                                                       aCAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAac                                                     >  3:199004/1‑149 (MQ=255)                                                                                           ttttTGTGATACTGGCACGCGCGAGTTGCGCCAGACGGGTCCCAGCGATAGGCAAGACTGGACCAAGCTGCTGAATCAGGGGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGCTCAATGTGTTGATGCTTTTTTTCAGCAaccacc                                                 <  8:229355/149‑1 (MQ=255)                                                                                           ttttAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCGCAGGATGGTTTTCTGCAaccacc                                                 <  2:485875/149‑1 (MQ=255)                                                                                                 tGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATAc                                           >  6:326919/1‑149 (MQ=255)                                                                                                   aTACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCTGCACCCACCGCATACTc                                         >  1:476626/1‑149 (MQ=255)                                                                                                   aTACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGc                                               <  4:74442/143‑1 (MQ=255)                                                                                                   aTACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGc                                               >  3:74442/1‑143 (MQ=255)                                                                                                   aTACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTc                                         <  3:415615/149‑1 (MQ=255)                                                                                                    tACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTcc                                        <  1:261168/149‑1 (MQ=255)                                                                                                      cTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTccc                                       <  2:440554/148‑1 (MQ=255)                                                                                                       tGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGAt                                                                                 >  8:251346/1‑105 (MQ=255)                                                                                                       tGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGAt                                                                                 <  7:251346/105‑1 (MQ=255)                                                                                                       tGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCta                                     >  1:258512/1‑149 (MQ=255)                                                                                                       tGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCta                                     >  4:74680/1‑149 (MQ=255)                                                                                                         gCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCtat                                    <  3:321/148‑1 (MQ=255)                                                                                                              aGCCAGTTGCGCCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTc                              <  2:28277/149‑1 (MQ=255)                                                                                                                      gcgcCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTcc                       <  7:279568/148‑1 (MQ=255)                                                                                                                       cgcCAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGa                     <  2:258512/149‑1 (MQ=255)                                                                                                                         ccAGACGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGaaa                   >  7:381281/1‑149 (MQ=255)                                                                                                                             aCGGGAAAGATCGATCCGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAaataat               <  5:343039/149‑1 (MQ=255)                                                                                                                             aCGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAaataat               >  2:193993/1‑149 (MQ=255)                                                                                                                             aCGGGAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAaataat               <  3:74680/149‑1 (MQ=255)                                                                                                                               gggAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCt             >  2:428937/1‑149 (MQ=255)                                                                                                                                 gAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTc                              <  5:202656/130‑1 (MQ=255)                                                                                                                                 gAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTc                              >  6:202656/1‑130 (MQ=255)                                                                                                                                 gAAAGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTg            >  3:80244/1‑148 (MQ=255)                                                                                                                                  aaaGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTg            >  8:249823/1‑147 (MQ=255)                                                                                                                                    aGATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAgg        >  8:270783/1‑149 (MQ=255)                                                                                                                                     gctCGATACGCAAGCCTGGACCAAGCTGATTAATCAGGCGATAAACCGCGCCCGCGTTGGTCGGCTTTATTTGATCAATGTGCCCAGGCTGGTTTGCCGCACCCCCCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTCGGc       >  3:283220/3‑149 (MQ=255)                                                                                                                                     gATCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGc       >  2:102459/1‑149 (MQ=255)                                                                                                                                      aTCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCt      <  8:38677/149‑1 (MQ=255)                                                                                                                                       tCGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTa     <  1:90572/149‑1 (MQ=255)                                                                                                                                       gcGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTa     <  5:172044/148‑1 (MQ=255)                                                                                                                                        cGATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTAt    >  8:195532/1‑149 (MQ=255)                                                                                                                                         gATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTAt    <  1:102459/148‑1 (MQ=255)                                                                                                                                         gATACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATg   <  2:14907/149‑1 (MQ=255)                                                                                                                                          aTACGCAAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATgg  <  7:270783/149‑1 (MQ=255)                                                                                                                                                |                                                                                                                                               CCACCAGCCCCACCGCCGGACGGCCACGGTTCCCCGCTTCTTTGATTTCCAGCTCTTGCACCAGGTGTGCTTCGAGCATCTCACGGACAATTTTAGTGATACTGGCAGGAGCCAGTTGCGCCAGACGGGAAAGATCGATACGCGAGACTGGACCAAGCTGATCAATCAGGCGATAAACCGCGCCCGCGTTGGTCTGCTTTATTTGATCAATGTGCCCAGGCTGGTTTTCAGCAACCACCGCATACTCCCTATATTTTCGCGCTCCGAAATAATCTGTAGGCTATGG  >  NC_000913/1668327‑1668612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑