Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,509,494 A→G 5.1% V107A (GTG→GCG)  yhfW ← phosphopentomutase‑related metalloenzyme superfamily protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,509,4940AG 5.1% 245.8 / 3.6 78V107A (GTG→GCG) yhfWphosphopentomutase‑related metalloenzyme superfamily protein
Reads supporting (aligned to +/- strand):  ref base A (38/36);  new base G (2/2);  total (40/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.06e-01

TAATGTTATAGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGCCTGGCGCAT  >  NC_000913/3509351‑3509638
                                                                                                                                               |                                                                                                                                                
tAATGTTATAGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATc                                                                                                                                              >  1:129388/1‑148 (MQ=255)
 aaTGTTATAGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATcg                                                                                                                                             >  2:149044/1‑148 (MQ=255)
    gTTATAGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATcgcg                                                                                                                                           >  5:80474/1‑147 (MQ=255)
      tataGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGaa                                                                                                                                         >  2:192108/1‑147 (MQ=255)
       attgACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGaa                                                                                                                                         >  8:311528/4‑146 (MQ=255)
       ataGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGaaa                                                                                                                                        <  6:80474/147‑1 (MQ=255)
           aCCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGc                                                                                                                                    <  2:70436/147‑1 (MQ=255)
              tGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATcc                                                                                                                                >  3:226286/1‑148 (MQ=255)
              tGGCCTAAATCCGCCTCGAGAATATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCCCCACGGCGATACACCTCCCAGACAGCGGAAACTAATGCCTGCTCAACACCGTCAATCACATCGCGAAAAGGCATcc                                                                                                                                >  8:314830/1‑148 (MQ=255)
               ggCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCg                                                                                                                               >  1:208534/1‑148 (MQ=255)
               ggCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCg                                                                                                                               >  4:139445/1‑148 (MQ=255)
               ggCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCg                                                                                                                               >  4:259659/1‑148 (MQ=255)
                    aaaTCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAgcg                                                                                                                          >  5:60937/1‑148 (MQ=255)
                    aaaTACGCCTCGAGATTATCGCAAATCTCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGATCCACCTGCCAGCGAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAgcg                                                                                                                          >  8:389309/1‑148 (MQ=255)
                     aaTCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATc                                                                                                                                 >  3:354615/1‑140 (MQ=255)
                     aaTCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATc                                                                                                                                 <  4:354615/140‑1 (MQ=255)
                     aaTCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAgcg                                                                                                                          <  1:192108/147‑1 (MQ=255)
                       tCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAGAAGGCATCCGCAgcggcg                                                                                                                       >  8:211169/1‑148 (MQ=255)
                        ccGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGAAGATCATCGCCACGCCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACAACGCGAAAAGGCATCCGCAGcggcgg                                                                                                                      >  1:273325/1‑148 (MQ=255)
                         cGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGt                                                                                                                     <  2:129388/148‑1 (MQ=255)
                          gCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTa                                                                                                                    <  5:139167/148‑1 (MQ=255)
                          gCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTACTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTa                                                                                                                    <  5:275690/148‑1 (MQ=255)
                            cTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAAc                                                                                                                  <  4:226286/148‑1 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTaa                                                                                                                   <  4:181647/145‑1 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTaa                                                                                                                   <  8:97946/145‑1 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTaa                                                                                                                   >  7:97946/1‑145 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTaa                                                                                                                   >  3:181647/1‑145 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTa                                                                                                                    <  8:327702/144‑1 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTa                                                                                                                    >  7:327702/1‑144 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACgg                                                                                                                >  5:289730/1‑148 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACgg                                                                                                                >  5:217338/1‑148 (MQ=255)
                              cGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACgg                                                                                                                >  3:198951/1‑148 (MQ=255)
                                agaTTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGgc                                                                                                              >  3:310918/1‑148 (MQ=255)
                                    tATCGCCAATCGCAACCGCCTGATTGACACACATAAATTGCAGATAAACCACACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCATACTCAACACGCTCAATCACATCGCGAAAAGCCATCCCCAGCGGCGGTAACGGgcgctt                                                                                                          >  7:349949/1‑146 (MQ=255)
                                              cGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAAtt                                                                                                >  3:72337/1‑148 (MQ=255)
                                               gCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAAttt                                                                                               <  4:72337/148‑1 (MQ=255)
                                                 aaCCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTcc                                                                                             <  4:310918/148‑1 (MQ=255)
                                                 aaCCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTcc                                                                                             <  6:60937/148‑1 (MQ=255)
                                                  aCCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCt                                                                                            <  6:217338/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGATATTGCAGATCATCCCCACGGCGCTCCACCTGCCAGCCAGCGGATACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCTCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTGCtt                                                                                           <  8:349949/148‑2 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTGCAGCTGCCAGACAGCGGAATGTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGGGCGTGCCTAAAATTTCCTg                                                                                           <  7:314830/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGACAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  7:211169/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAACAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  7:311528/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  3:139445/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  7:389309/148‑1 (MQ=255)
                                                   ccGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  6:289730/148‑1 (MQ=255)
                                                     gCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGAt                                                                                         <  1:149044/148‑1 (MQ=255)
                                                       cTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCCGATGc                                                                                       <  3:259659/148‑1 (MQ=255)
                                                         gATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCGCATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAAttt                                                                                               <  7:328612/138‑1 (MQ=255)
                                                         gATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCGCATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAAttt                                                                                               >  8:328612/1‑138 (MQ=255)
                                                              aCCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATaa                                                                                <  2:208534/148‑1 (MQ=255)
                                                                  acaGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGc                                                                                                                                    >  7:373013/1‑92 (MQ=255)
                                                                  acaGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGc                                                                                                                                    <  8:373013/92‑1 (MQ=255)
                                                                  acaGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  8:5349/133‑1 (MQ=255)
                                                                  acaGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           >  7:5349/1‑133 (MQ=255)
                                                                   caGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTa                                                                           <  4:198951/148‑1 (MQ=255)
                                                                                    cGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTg                                                          >  4:275814/1‑148 (MQ=255)
                                                                                       cACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCGCATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAg                                                                              <  7:12224/125‑1 (MQ=255)
                                                                                       cACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCGCATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAg                                                                              >  8:12224/1‑125 (MQ=255)
                                                                                         cGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGct                                                     <  3:275814/148‑1 (MQ=255)
                                                                                          ggCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           >  2:249018/1‑109 (MQ=255)
                                                                                          ggCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTg                                                                                           <  1:249018/109‑1 (MQ=255)
                                                                                          ggCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCg                                                                        >  2:268340/1‑128 (MQ=255)
                                                                                          ggCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCg                                                                        <  1:268340/128‑1 (MQ=255)
                                                                                                            cagcGGAAACTAATGCCTGCTCAACACGGTCACTCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGACACCTTCATGTTGCAGCTCTGCCACGCCCAAGGATGc                                  >  6:266654/1‑148 (MQ=255)
                                                                                                                       aaTGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTc                                                                                              <  2:285696/77‑1 (MQ=255)
                                                                                                                       aaTGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTc                                                                                              >  1:285696/1‑77 (MQ=255)
                                                                                                                       aaTGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACg                       >  1:1367/1‑148 (MQ=255)
                                                                                                                              gCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGTGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCa                 >  2:2265/1‑147 (MQ=255)
                                                                                                                              gCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGct                                                     >  8:20625/1‑111 (MQ=255)
                                                                                                                              gCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGct                                                     <  7:20625/111‑1 (MQ=255)
                                                                                                                                tCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTa                                                                           <  8:25014/87‑1 (MQ=255)
                                                                                                                                tCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTa                                                                           >  7:25014/1‑87 (MQ=255)
                                                                                                                                tCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCatat              >  2:237874/1‑148 (MQ=255)
                                                                                                                                    cacGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGcc          <  4:28177/148‑1 (MQ=255)
                                                                                                                                    cacGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGcc          >  3:28177/1‑148 (MQ=255)
                                                                                                                                          cAATCACATCGCGCAACGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGAATCGCCACCTTCATTTTGCAGATCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGCCTGgcgc    <  2:273325/148‑1 (MQ=255)
                                                                                                                                             tCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGCCTGGCGCAt  >  1:9053/1‑147 (MQ=255)
                                                                                                                                               |                                                                                                                                                
TAATGTTATAGACCTGGCCTAAATCCGCCTCGAGATTATCGCCAATCGCAACCGCCTGATTGACCCACAGAAATTGCAGATCATCGCCACGGCGCTCCACCTGCCAGCCAGCGGAAACTAATGCCTGCTCAACACGGTCAATCACATCGCGAAAAGGCATCCGCAGCGGCGGTAACGGGCGCGTGCCTAAAATTTCCTGATGCCCCATAAAGGTATCGCCACCTTCATGTTGCAGCTCTGCCACGCCCCAGGTTGCGGAATCTGACGGCTGCATATCGCCTGGCGCAT  >  NC_000913/3509351‑3509638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑